Gene description for Prps1
Gene name phosphoribosyl pyrophosphate synthetase 1
Gene symbol Prps1
Other names/aliases 2310010D17Rik
C76571
C76678
PRS-I
Prps-1
Species Mus musculus
 Database cross references - Prps1
ExoCarta ExoCarta_19139
Vesiclepedia VP_19139
Entrez Gene 19139
UniProt Q9D7G0  
 Prps1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prps1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    ribose phosphate diphosphokinase activity GO:0004749 IBA
    ribose phosphate diphosphokinase activity GO:0004749 IDA
    ribose phosphate diphosphokinase activity GO:0004749 ISO
    ribose phosphate diphosphokinase activity GO:0004749 ISS
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    AMP binding GO:0016208 ISO
    kinase activity GO:0016301 IEA
    GDP binding GO:0019003 ISO
    carbohydrate binding GO:0030246 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    ADP binding GO:0043531 ISO
Biological Process
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IBA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IDA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IEA
    pentose-phosphate shunt GO:0006098 IMP
    purine nucleobase metabolic process GO:0006144 ISO
    purine nucleotide biosynthetic process GO:0006164 IBA
    purine nucleotide biosynthetic process GO:0006164 ISO
    nervous system development GO:0007399 ISO
    ribonucleoside monophosphate biosynthetic process GO:0009156 IEA
    nucleotide biosynthetic process GO:0009165 IEA
    urate biosynthetic process GO:0034418 ISO
    hypoxanthine biosynthetic process GO:0046101 ISO
Subcellular Localization
    ribose phosphate diphosphokinase complex GO:0002189 IBA
    ribose phosphate diphosphokinase complex GO:0002189 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Prps1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prps1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg16l1  
Affinity Capture-MS Mus musculus
2 Dkc1 245474
Co-fractionation Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Ddx47  
Co-fractionation Mus musculus
5 Rsl1d1 66409
Co-fractionation Mus musculus
6 Trmt11  
Co-fractionation Mus musculus
7 Impdh1  
Co-fractionation Mus musculus
8 Park2  
Affinity Capture-Western Mus musculus
9 Trmt6  
Co-fractionation Mus musculus
View the network image/svg+xml



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