Gene description for Fth1
Gene name ferritin heavy chain 1
Gene symbol Fth1
Other names/aliases FHC
Fth
HFt
MFH
Species Mus musculus
 Database cross references - Fth1
ExoCarta ExoCarta_14319
Vesiclepedia VP_14319
Entrez Gene 14319
UniProt P09528  
 Fth1 identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fth1
Molecular Function
    ferroxidase activity GO:0004322 IEA
    ferroxidase activity GO:0004322 ISO
    iron ion binding GO:0005506 IDA
    ferrous iron binding GO:0008198 IBA
    ferrous iron binding GO:0008198 IEA
    ferrous iron binding GO:0008198 ISO
    ferric iron binding GO:0008199 IBA
    ferric iron binding GO:0008199 IEA
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    iron ion sequestering activity GO:0140315 IEA
Biological Process
    iron ion transport GO:0006826 IBA
    iron ion transport GO:0006826 IDA
    intracellular iron ion homeostasis GO:0006879 IEA
    immune response GO:0006955 ISS
    negative regulation of cell population proliferation GO:0008285 ISS
    negative regulation of fibroblast proliferation GO:0048147 IEA
    negative regulation of fibroblast proliferation GO:0048147 ISO
    negative regulation of ferroptosis GO:0110076 ISO
    negative regulation of ferroptosis GO:0110076 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    autophagosome GO:0005776 IEA
    cytosol GO:0005829 IDA
    membrane GO:0016020 IDA
    autolysosome GO:0044754 ISO
    endocytic vesicle lumen GO:0071682 TAS
 Experiment description of studies that identified Fth1 in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
5
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fth1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tollip 54473
Co-fractionation Mus musculus
2 Wdyhv1  
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Uchl5 56207
Co-fractionation Mus musculus
5 Spp1 20750
Co-fractionation Mus musculus
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