Gene description for Rab8b
Gene name RAB8B, member RAS oncogene family
Gene symbol Rab8b
Other names/aliases 5930437D16
D330025I23Rik
Species Mus musculus
 Database cross references - Rab8b
ExoCarta ExoCarta_235442
Vesiclepedia VP_235442
Entrez Gene 235442
UniProt P61028  
 Rab8b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab8b
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    signaling receptor binding GO:0005102 IEA
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    TPR domain binding GO:0030911 IEA
    TPR domain binding GO:0030911 ISO
Biological Process
    exocytosis GO:0006887 IBA
    antigen processing and presentation GO:0019882 IEA
    antigen processing and presentation GO:0019882 ISO
    positive regulation of cell projection organization GO:0031346 IEA
    positive regulation of cell projection organization GO:0031346 ISO
    endocytic recycling GO:0032456 IBA
    protein import into peroxisome membrane GO:0045046 IEA
    protein import into peroxisome membrane GO:0045046 ISO
    positive regulation of corticotropin secretion GO:0051461 IEA
    positive regulation of corticotropin secretion GO:0051461 ISO
    cell-substrate junction organization GO:0150115 ISO
    cell-substrate junction organization GO:0150115 ISS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    endosome GO:0005768 IBA
    peroxisomal membrane GO:0005778 IEA
    peroxisomal membrane GO:0005778 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    synaptic vesicle GO:0008021 IBA
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    trans-Golgi network transport vesicle GO:0030140 IBA
    phagocytic vesicle membrane GO:0030670 IEA
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    cell tip GO:0051286 IEA
    cell tip GO:0051286 ISO
    presynapse GO:0098793 IEA
    presynapse GO:0098793 ISO
 Experiment description of studies that identified Rab8b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab8b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vim 22352
Co-fractionation Mus musculus
2 Vamp2  
Co-fractionation Mus musculus
3 Tuba4a 22145
Co-fractionation Mus musculus
4 Rab1 19324
Co-fractionation Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
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