Gene description for Rab1
Gene name RAB1, member RAS oncogene family
Gene symbol Rab1
Other names/aliases Gtbp
Rab-1
Rab1A
Ypt1
mKIAA3012
Species Mus musculus
 Database cross references - Rab1
ExoCarta ExoCarta_19324
Vesiclepedia VP_19324
Entrez Gene 19324
UniProt P62821  
 Rab1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab1
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISS
    acrosome assembly GO:0001675 ISS
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 TAS
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISO
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISO
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISS
    endocytosis GO:0006897 ISO
    endocytosis GO:0006897 ISS
    autophagy GO:0006914 ISO
    autophagy GO:0006914 ISS
    Golgi organization GO:0007030 ISO
    Golgi organization GO:0007030 ISS
    cell migration GO:0016477 IMP
    cell migration GO:0016477 ISO
    virion assembly GO:0019068 IEA
    virion assembly GO:0019068 ISO
    growth hormone secretion GO:0030252 ISO
    growth hormone secretion GO:0030252 ISS
    melanosome transport GO:0032402 IMP
    positive regulation of interleukin-8 production GO:0032757 ISO
    positive regulation of interleukin-8 production GO:0032757 ISS
    substrate adhesion-dependent cell spreading GO:0034446 IMP
    defense response to bacterium GO:0042742 ISO
    defense response to bacterium GO:0042742 ISS
    vesicle transport along microtubule GO:0047496 ISO
    vesicle transport along microtubule GO:0047496 ISS
    COPII-coated vesicle cargo loading GO:0090110 IEA
    COPII-coated vesicle cargo loading GO:0090110 ISO
    positive regulation of glycoprotein metabolic process GO:1903020 IEA
    positive regulation of glycoprotein metabolic process GO:1903020 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IEA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 IEA
    melanosome GO:0042470 IDA
    neuronal cell body GO:0043025 ISO
    synapse GO:0045202 ISO
    presynapse GO:0098793 ISO
    presynapse GO:0098793 ISO
 Experiment description of studies that identified Rab1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kcnma1  
Affinity Capture-MS Mus musculus
2 Tlr4  
Affinity Capture-Western Mus musculus
3 Syngap1  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Rab11b 19326
Co-fractionation Mus musculus
6 Kctd13  
Affinity Capture-MS Mus musculus
7 Mapt  
Affinity Capture-MS Mus musculus
8 Rnf115  
Affinity Capture-Western Mus musculus
9 Rab8b 235442
Co-fractionation Mus musculus
10 Unk  
Affinity Capture-RNA Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Gdi1 14567
Affinity Capture-Western Mus musculus
13 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
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