Gene description for Rab11b
Gene name RAB11B, member RAS oncogene family
Gene symbol Rab11b
Other names/aliases A730055L17Rik
Species Mus musculus
 Database cross references - Rab11b
ExoCarta ExoCarta_19326
Vesiclepedia VP_19326
Entrez Gene 19326
UniProt P46638  
 Rab11b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Microglia 16081791    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab11b
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IMP
    GTPase activity GO:0003924 ISO
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
Biological Process
    receptor recycling GO:0001881 IMP
    endocytosis GO:0006897 IMP
    melanosome transport GO:0032402 IMP
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    transferrin transport GO:0033572 IMP
    insulin secretion involved in cellular response to glucose stimulus GO:0035773 IMP
    regulation of monoatomic anion transport GO:0044070 ISO
    regulation of monoatomic anion transport GO:0044070 ISS
    constitutive secretory pathway GO:0045054 IBA
    constitutive secretory pathway GO:0045054 ISO
    constitutive secretory pathway GO:0045054 ISS
    regulated exocytosis GO:0045055 IBA
    regulated exocytosis GO:0045055 IMP
    regulated exocytosis GO:0045055 ISO
    cellular response to acidic pH GO:0071468 ISO
    cellular response to acidic pH GO:0071468 ISS
    establishment of protein localization to membrane GO:0090150 IEA
    establishment of protein localization to membrane GO:0090150 ISO
    amyloid-beta clearance by transcytosis GO:0150093 IEA
    amyloid-beta clearance by transcytosis GO:0150093 ISO
    regulation of protein localization to cell surface GO:2000008 ISO
    regulation of protein localization to cell surface GO:2000008 ISS
    regulation of endocytic recycling GO:2001135 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HDA
    Golgi apparatus GO:0005794 IBA
    synaptic vesicle GO:0008021 ISO
    synaptic vesicle GO:0008021 ISS
    phagocytic vesicle membrane GO:0030670 IEA
    synaptic vesicle membrane GO:0030672 IEA
    synaptic vesicle membrane GO:0030672 ISO
    synaptic vesicle membrane GO:0030672 ISO
    cytoplasmic vesicle GO:0031410 ISO
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    recycling endosome GO:0055037 IBA
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IDA
 Experiment description of studies that identified Rab11b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
6
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab11b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rab1 19324
Co-fractionation Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Rpa1 68275
Co-fractionation Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Rpa3  
Co-fractionation Mus musculus
6 Kcnma1  
Affinity Capture-MS Mus musculus
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