Gene description for Anxa2
Gene name annexin A2
Gene symbol Anxa2
Other names/aliases AW215814
Cal1h
Species Mus musculus
 Database cross references - Anxa2
ExoCarta ExoCarta_12306
Vesiclepedia VP_12306
Entrez Gene 12306
UniProt P07356  
 Anxa2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 10545503    
Embryonic fibroblasts 18494037    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Microglia 16081791    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Anxa2
Molecular Function
    phosphatidylserine binding GO:0001786 IBA
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    serine-type endopeptidase inhibitor activity GO:0004867 ISO
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 IEA
    calcium-dependent phospholipid binding GO:0005544 ISO
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    cytoskeletal protein binding GO:0008092 IEA
    phospholipase A2 inhibitor activity GO:0019834 IEA
    phospholipase A2 inhibitor activity GO:0019834 ISO
    small GTPase binding GO:0031267 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    S100 protein binding GO:0044548 IEA
    S100 protein binding GO:0044548 ISO
    bone sialoprotein binding GO:0044730 ISO
    calcium-dependent protein binding GO:0048306 IEA
Biological Process
    angiogenesis GO:0001525 IMP
    membrane raft assembly GO:0001765 IEA
    positive regulation of receptor recycling GO:0001921 IMP
    positive regulation of protein phosphorylation GO:0001934 ISO
    negative regulation of receptor internalization GO:0002091 ISO
    vesicle budding from membrane GO:0006900 IEA
    cell adhesion GO:0007155 IMP
    cell-matrix adhesion GO:0007160 IMP
    body fluid secretion GO:0007589 ISO
    positive regulation of plasminogen activation GO:0010756 ISO
    response to activity GO:0014823 IMP
    collagen fibril organization GO:0030199 IMP
    lung development GO:0030324 IMP
    positive regulation of vesicle fusion GO:0031340 IEA
    negative regulation of low-density lipoprotein particle receptor catabolic process GO:0032804 IMP
    osteoclast development GO:0036035 IEA
    fibrinolysis GO:0042730 IMP
    mRNA transcription by RNA polymerase II GO:0042789 IDA
    positive regulation of vacuole organization GO:0044090 IEA
    positive regulation of exocytosis GO:0045921 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    regulation of neurogenesis GO:0050767 IDA
    positive regulation of binding GO:0051099 IMP
    cellular response to acid chemical GO:0071229 IDA
    protein localization to plasma membrane GO:0072659 ISO
    epithelial cell apoptotic process GO:1904019 IMP
    positive regulation of low-density lipoprotein particle clearance GO:1905581 IMP
    positive regulation of low-density lipoprotein particle receptor binding GO:1905597 IMP
    positive regulation of low-density lipoprotein receptor activity GO:1905599 IMP
    positive regulation of receptor-mediated endocytosis involved in cholesterol transport GO:1905602 IBA
    positive regulation of receptor-mediated endocytosis involved in cholesterol transport GO:1905602 IMP
    positive regulation of plasma membrane repair GO:1905686 NAS
Subcellular Localization
    cornified envelope GO:0001533 IDA
    stress fiber GO:0001725 ISS
    ruffle GO:0001726 ISO
    basement membrane GO:0005604 IEA
    extracellular space GO:0005615 HDA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    endosome GO:0005768 ISO
    early endosome GO:0005769 IDA
    lipid droplet GO:0005811 IEA
    lipid droplet GO:0005811 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell cortex GO:0005938 ISO
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    vesicle membrane GO:0012506 IBA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    basolateral plasma membrane GO:0016323 IDA
    nuclear matrix GO:0016363 NAS
    cell junction GO:0030054 ISS
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    vesicle GO:0031982 ISO
    protein-containing complex GO:0032991 ISO
    myelin sheath adaxonal region GO:0035749 IDA
    sarcolemma GO:0042383 IDA
    melanosome GO:0042470 IEA
    Schmidt-Lanterman incisure GO:0043220 IDA
    macropinosome GO:0044354 ISO
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    RNA polymerase II transcription regulator complex GO:0090575 IPI
    RNA polymerase II transcription regulator complex GO:0090575 ISO
    plasma membrane protein complex GO:0098797 EXP
    plasma membrane protein complex GO:0098797 IPI
    plasma membrane protein complex GO:0098797 ISO
    AnxA2-p11 complex GO:1990665 IEA
    AnxA2-p11 complex GO:1990665 ISO
    PCSK9-AnxA2 complex GO:1990667 ISO
    PCSK9-AnxA2 complex GO:1990667 ISS
 Experiment description of studies that identified Anxa2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 3
MISEV standards
✔
IEM
Biophysical techniques
✔
HSP84|HSC73|MHCII|CD9|mac-1
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors "Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S."
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
3
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
5
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
6
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
11
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
12
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
13
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Anxa2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pou5f1  
Affinity Capture-MS Mus musculus
2 Vim 22352
Co-fractionation Mus musculus
3 Hltf  
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
Co-crystal Structure Mus musculus
4 Nphp4  
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Tfe3  
Affinity Capture-MS Mus musculus
7 S100A10 6281
Affinity Capture-MS Homo sapiens
8 PLOD1 5351
Affinity Capture-MS Homo sapiens
9 Ahnak2  
Protein-peptide Mus musculus
10 Fbxw10  
Affinity Capture-MS Mus musculus
11 S100a11 20195
Co-fractionation Mus musculus
12 Tmem173  
Proximity Label-MS Mus musculus
13 PPL 5493
Affinity Capture-MS Homo sapiens
14 PLOD2 5352
Affinity Capture-MS Homo sapiens
15 PLOD3 8985
Affinity Capture-MS Homo sapiens
16 Agap2  
Affinity Capture-MS Mus musculus
17 Ahnak  
Protein-peptide Mus musculus
Co-crystal Structure Mus musculus
18 AHNAK 79026
Affinity Capture-MS Homo sapiens
19 Invs  
Affinity Capture-MS Mus musculus
20 S100a10 20194
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
21 AHNAK2 113146
Affinity Capture-MS Homo sapiens
22 COLGALT1 79709
Affinity Capture-MS Homo sapiens
23 S100a6 20200
Co-fractionation Mus musculus
24 Nphp1  
Affinity Capture-MS Mus musculus
25 Dysf  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
26 Fancd2  
Affinity Capture-MS Mus musculus
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