Experiment description of studies that identified NCCRP1 in sEVs

1 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 66 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|Alix|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

5 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for NCCRP1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ERCC3   Affinity Capture-MS Homo sapiens 2 POT1   Affinity Capture-MS Homo sapiens 3 RNF38   Affinity Capture-MS Homo sapiens 4 MRM1   Affinity Capture-MS Homo sapiens 5 SLX4   Affinity Capture-MS Homo sapiens 6 RRM2 6241 Affinity Capture-MS Homo sapiens 7 FAM153B   Affinity Capture-MS Homo sapiens 8 STK16 8576 Affinity Capture-MS Homo sapiens 9 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 10 CCNC   Affinity Capture-MS Homo sapiens 11 SLC25A6 293 Affinity Capture-MS Homo sapiens 12 YAF2   Affinity Capture-MS Homo sapiens 13 DDI1   Two-hybrid Homo sapiens 14 ATP6AP2 10159 Affinity Capture-MS Homo sapiens 15 FANCD2   Affinity Capture-MS Homo sapiens 16 PIGT 51604 Affinity Capture-MS Homo sapiens 17 AGPAT1 10554 Affinity Capture-MS Homo sapiens 18 CABS1   Affinity Capture-MS Homo sapiens 19 FCF1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 NT5C1A   Affinity Capture-MS Homo sapiens 21 POLR2L 5441 Affinity Capture-MS Homo sapiens 22 SH2D3A   Affinity Capture-MS Homo sapiens 23 TBC1D22B   Affinity Capture-MS Homo sapiens 24 COQ5   Affinity Capture-MS Homo sapiens 25 SLC35F2 54733 Affinity Capture-MS Homo sapiens 26 B3GNT2 10678 Affinity Capture-MS Homo sapiens 27 CD274 29126 Affinity Capture-MS Homo sapiens 28 UCP2   Affinity Capture-MS Homo sapiens 29 ZSCAN12   Affinity Capture-MS Homo sapiens 30 CDK15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 C1orf35   Affinity Capture-MS Homo sapiens 32 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 33 ZIC1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 ZC3HC1   Affinity Capture-MS Homo sapiens 35 WTAP 9589 Affinity Capture-MS Homo sapiens 36 HNRNPA1 3178 Affinity Capture-MS Homo sapiens 37 ZDHHC19   Affinity Capture-MS Homo sapiens 38 RPS19BP1   Affinity Capture-MS Homo sapiens 39 FTH1 2495 Affinity Capture-MS Homo sapiens 40 ST7   Affinity Capture-MS Homo sapiens 41 BPHL 670 Affinity Capture-MS Homo sapiens 42 ST6GALNAC6 30815 Affinity Capture-MS Homo sapiens 43 FNDC5   Affinity Capture-MS Homo sapiens 44 PI4KA 5297 Affinity Capture-MS Homo sapiens 45 C21orf59 56683 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 MBNL1 4154 Affinity Capture-MS Homo sapiens 47 ESR1   Affinity Capture-MS Homo sapiens 48 RTCA 8634 Affinity Capture-MS Homo sapiens 49 DPPA4   Affinity Capture-MS Homo sapiens 50 PLEKHB2 55041 Affinity Capture-MS Homo sapiens 51 PTDSS1 9791 Affinity Capture-MS Homo sapiens 52 PI4KAP1   Affinity Capture-MS Homo sapiens 53 CCDC51 79714 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 54 CCR1   Affinity Capture-MS Homo sapiens 55 FANCG 2189 Affinity Capture-MS Homo sapiens 56 AMTN   Affinity Capture-MS Homo sapiens 57 TEFM   Affinity Capture-MS Homo sapiens 58 SARAF   Affinity Capture-MS Homo sapiens 59 MCM2 4171 Affinity Capture-MS Homo sapiens 60 NUDT3 11165 Affinity Capture-MS Homo sapiens 61 PINK1   Affinity Capture-MS Homo sapiens 62 GPR18   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 GOT1 2805 Affinity Capture-MS Homo sapiens 64 TRIM21 6737 Affinity Capture-MS Homo sapiens 65 E2F6 1876 Affinity Capture-MS Homo sapiens 66 SSUH2   Affinity Capture-MS Homo sapiens 67 PDE4DIP 9659 Affinity Capture-MS Homo sapiens 68 ALDH3A1 218 Affinity Capture-MS Homo sapiens 69 DUSP21   Affinity Capture-MS Homo sapiens 70 HCN1   Affinity Capture-MS Homo sapiens 71 APTX   Affinity Capture-MS Homo sapiens 72 UPK1A 11045 Affinity Capture-MS Homo sapiens 73 MRPL38   Affinity Capture-MS Homo sapiens 74 GABPA 2551 Affinity Capture-MS Homo sapiens 75 DDX39B 7919 Affinity Capture-MS Homo sapiens 76 C18orf21   Affinity Capture-MS Homo sapiens 77 ZDHHC6   Affinity Capture-MS Homo sapiens 78 STX17 55014 Affinity Capture-MS Homo sapiens 79 P2RX6   Affinity Capture-MS Homo sapiens 80 CYLD   Affinity Capture-MS Homo sapiens 81 SOX2   Affinity Capture-MS Homo sapiens 82 PHF11 51131 Affinity Capture-MS Homo sapiens View the network

Pathways in which NCCRP1 is involved

No pathways found