Gene description for MARK3
Gene name MAP/microtubule affinity-regulating kinase 3
Gene symbol MARK3
Other names/aliases CTAK1
KP78
PAR1A
Par-1a
Species Homo sapiens
 Database cross references - MARK3
ExoCarta ExoCarta_4140
Vesiclepedia VP_4140
Entrez Gene 4140
HGNC 6897
MIM 602678
UniProt P27448  
 MARK3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MARK3
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 NAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    tau protein binding GO:0048156 NAS
    tau-protein kinase activity GO:0050321 IBA
    tau-protein kinase activity GO:0050321 IMP
    tau-protein kinase activity GO:0050321 NAS
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IMP
    peptidyl-serine phosphorylation GO:0018105 IDA
    positive regulation of protein binding GO:0032092 IDA
    negative regulation of hippo signaling GO:0035331 IDA
    intracellular signal transduction GO:0035556 IBA
    peptidyl-serine autophosphorylation GO:0036289 IDA
    negative regulation of protein localization to nucleus GO:1900181 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    dendrite GO:0030425 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MARK3 in sEVs
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MARK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
3 PRKAA1 5562
Affinity Capture-MS Homo sapiens
4 DLG5 9231
Affinity Capture-MS Homo sapiens
5 HDAC4  
Affinity Capture-Western Homo sapiens
6 UTRN 7402
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 RASGRF1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 KSR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
10 MARCKS 4082
Proximity Label-MS Homo sapiens
11 LAMP3  
Proximity Label-MS Homo sapiens
12 GJA1 2697
Proximity Label-MS Homo sapiens
13 PKP2 5318
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
14 CTBP2 1488
Affinity Capture-MS Homo sapiens
15 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
16 TTR 7276
Two-hybrid Homo sapiens
17 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
18 GJD3  
Proximity Label-MS Homo sapiens
19 LAMP2 3920
Proximity Label-MS Homo sapiens
20 CLTB 1212
Proximity Label-MS Homo sapiens
21 STX4 6810
Proximity Label-MS Homo sapiens
22 SOX2  
Affinity Capture-MS Homo sapiens
23 GSK3A 2931
Affinity Capture-MS Homo sapiens
24 APEX1 328
Affinity Capture-RNA Homo sapiens
25 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 B3GAT1  
Proximity Label-MS Homo sapiens
27 MLLT4 4301
Proximity Label-MS Homo sapiens
28 SOGA1 140710
Affinity Capture-MS Homo sapiens
29 COL4A3BP 10087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ACVR2B  
Affinity Capture-MS Homo sapiens
32 MACC1 346389
Affinity Capture-MS Homo sapiens
33 STX6 10228
Proximity Label-MS Homo sapiens
34 TEX19  
Affinity Capture-MS Homo sapiens
35 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SFN 2810
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
37 MARK4  
Affinity Capture-MS Homo sapiens
38 RAB35 11021
Proximity Label-MS Homo sapiens
39 MYCN  
Affinity Capture-MS Homo sapiens
40 EBAG9 9166
Proximity Label-MS Homo sapiens
41 LAMTOR1 55004
Proximity Label-MS Homo sapiens
42 CLASP2 23122
Affinity Capture-MS Homo sapiens
43 MAP2 4133
Biochemical Activity Homo sapiens
44 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MANSC1 54682
Affinity Capture-MS Homo sapiens
46 PPIE 10450
Affinity Capture-MS Homo sapiens
47 CAPZA1 829
Affinity Capture-MS Homo sapiens
48 USP21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CXADR 1525
Proximity Label-MS Homo sapiens
50 SAAL1 113174
Two-hybrid Homo sapiens
51 TCEA2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
52 MEX3C 51320
Affinity Capture-MS Homo sapiens
53 LYN 4067
Proximity Label-MS Homo sapiens
54 MCAM 4162
Proximity Label-MS Homo sapiens
55 DNAJC5 80331
Proximity Label-MS Homo sapiens
56 AKR7L 246181
Affinity Capture-MS Homo sapiens
57 HDAC7  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
58 MAPT  
FRET Homo sapiens
59 CDH1 999
Proximity Label-MS Homo sapiens
60 PARD3 56288
Proximity Label-MS Homo sapiens
61 DIRAS3  
Proximity Label-MS Homo sapiens
62 NTRK1 4914
Affinity Capture-MS Homo sapiens
63 GPSM3  
Affinity Capture-MS Homo sapiens
64 CHGB 1114
Two-hybrid Homo sapiens
65 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CEP85L  
Affinity Capture-MS Homo sapiens
67 HDAC5 10014
Affinity Capture-Western Homo sapiens
68 FGF13  
Affinity Capture-MS Homo sapiens
69 VPS11 55823
Two-hybrid Homo sapiens
70 DNAJC5B  
Proximity Label-MS Homo sapiens
71 CNBP 7555
Affinity Capture-MS Homo sapiens
72 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
73 NUAK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 MITF  
Two-hybrid Homo sapiens
75 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
76 Myh9 17886
Affinity Capture-MS Mus musculus
77 Crtc2  
Biochemical Activity Mus musculus
78 RAB5A 5868
Proximity Label-MS Homo sapiens
79 PANX1 24145
Proximity Label-MS Homo sapiens
80 ECT2 1894
Affinity Capture-MS Homo sapiens
81 EPB41L4A 64097
Proximity Label-MS Homo sapiens
82 LAMP1 3916
Proximity Label-MS Homo sapiens
83 YWHAZ 7534
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 Dlg5  
Affinity Capture-MS Mus musculus
85 ARF6 382
Proximity Label-MS Homo sapiens
86 PNMA2  
Affinity Capture-MS Homo sapiens
87 TMEM74  
Affinity Capture-MS Homo sapiens
88 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
89 CDC25C  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
90 PRKACA 5566
Proximity Label-MS Homo sapiens
91 TADA2A  
Affinity Capture-MS Homo sapiens
92 RPA3 6119
Proximity Label-MS Homo sapiens
93 RAB9A 9367
Proximity Label-MS Homo sapiens
94 CDH19  
Affinity Capture-MS Homo sapiens
95 RHOB 388
Proximity Label-MS Homo sapiens
96 CTNNA1 1495
Proximity Label-MS Homo sapiens
97 PNPLA2  
Two-hybrid Homo sapiens
98 PPP2CB 5516
Affinity Capture-MS Homo sapiens
99 FLOT1 10211
Proximity Label-MS Homo sapiens
100 HECTD1 25831
Affinity Capture-MS Homo sapiens
101 MARK3 4140
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
102 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
103 FASN 2194
Negative Genetic Homo sapiens
104 NRROS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 TERF2  
Affinity Capture-MS Homo sapiens
106 PARP1 142
Proximity Label-MS Homo sapiens
107 EZR 7430
Proximity Label-MS Homo sapiens
108 LCK 3932
Proximity Label-MS Homo sapiens
109 ASB13  
Affinity Capture-Western Homo sapiens
110 PIM1  
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
111 C11orf52 91894
Proximity Label-MS Homo sapiens
112 LMAN1 3998
Proximity Label-MS Homo sapiens
113 PMPCB 9512
Two-hybrid Homo sapiens
114 ATP6V0C 527
Two-hybrid Homo sapiens
115 RAB2A 5862
Proximity Label-MS Homo sapiens
116 ALYREF 10189
Affinity Capture-MS Homo sapiens
117 MARK2 2011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 ZNF14  
Affinity Capture-MS Homo sapiens
119 TRIP4 9325
Affinity Capture-MS Homo sapiens
120 RAB11A 8766
Proximity Label-MS Homo sapiens
121 EPHA2 1969
Proximity Label-MS Homo sapiens
122 PTGES3 10728
Affinity Capture-MS Homo sapiens
123 KRAS 3845
Proximity Label-MS Homo sapiens
124 TMCC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 MTCL1 23255
Affinity Capture-MS Homo sapiens
126 RC3H1 149041
Affinity Capture-MS Homo sapiens
127 RPA2 6118
Proximity Label-MS Homo sapiens
128 Bcl7c  
Affinity Capture-MS Mus musculus
129 CAV1 857
Proximity Label-MS Homo sapiens
130 CPSF3 51692
Affinity Capture-MS Homo sapiens
131 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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