Gene description for CPSF3
Gene name cleavage and polyadenylation specific factor 3, 73kDa
Gene symbol CPSF3
Other names/aliases CPSF-73
CPSF73
Species Homo sapiens
 Database cross references - CPSF3
ExoCarta ExoCarta_51692
Vesiclepedia VP_51692
Entrez Gene 51692
HGNC 2326
MIM 606029
UniProt Q9UKF6  
 CPSF3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CPSF3
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISS
    RNA endonuclease activity GO:0004521 IBA
    RNA endonuclease activity GO:0004521 IMP
    RNA endonuclease activity GO:0004521 ISS
    5'-3' RNA exonuclease activity GO:0004534 IBA
    5'-3' RNA exonuclease activity GO:0004534 ISS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IMP
Biological Process
    mRNA 3'-end processing by stem-loop binding and cleavage GO:0006398 IBA
    mRNA 3'-end processing by stem-loop binding and cleavage GO:0006398 IDA
    mRNA 3'-end processing GO:0031124 IMP
    co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway GO:0180010 IDA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IBA
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IDA
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified CPSF3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CPSF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 RAD18  
Affinity Capture-MS Homo sapiens
4 PAFAH1B1 5048
Proximity Label-MS Homo sapiens
5 MARK3 4140
Affinity Capture-MS Homo sapiens
6 RIT1 6016
Negative Genetic Homo sapiens
7 DHX16 8449
Co-fractionation Homo sapiens
8 NUDT21 11051
Co-fractionation Homo sapiens
9 MECP2 4204
Affinity Capture-MS Homo sapiens
10 CTPS1 1503
Co-fractionation Homo sapiens
11 DNAAF2  
Affinity Capture-MS Homo sapiens
12 KIF21B 23046
Affinity Capture-MS Homo sapiens
13 MYCN  
Affinity Capture-MS Homo sapiens
14 CPSF1 29894
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 STUB1 10273
Affinity Capture-MS Homo sapiens
16 CSTF1 1477
Affinity Capture-MS Homo sapiens
17 UBE2H 7328
Affinity Capture-MS Homo sapiens
18 PAXIP1  
Affinity Capture-MS Homo sapiens
19 CDC73  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
20 SMARCAD1  
Affinity Capture-MS Homo sapiens
21 KIF14 9928
Affinity Capture-MS Homo sapiens
22 PCF11 51585
Co-fractionation Homo sapiens
23 CSTF2T  
Affinity Capture-MS Homo sapiens
24 FAS 355
Proximity Label-MS Homo sapiens
25 SSU72 29101
Affinity Capture-MS Homo sapiens
26 WWOX 51741
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
27 BAG2 9532
Affinity Capture-MS Homo sapiens
28 SIRT7  
Affinity Capture-MS Homo sapiens
29 KDM1B  
Affinity Capture-MS Homo sapiens
30 AURKA 6790
Affinity Capture-MS Homo sapiens
31 P4HA2 8974
Affinity Capture-MS Homo sapiens
32 PTBP1 5725
Co-fractionation Homo sapiens
33 GLTSCR2  
Affinity Capture-MS Homo sapiens
34 U2AF2 11338
Co-fractionation Homo sapiens
35 WDR33 55339
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SYMPK 8189
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 P4HA1 5033
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
39 C9orf72  
Affinity Capture-MS Homo sapiens
40 HSPB1 3315
Two-hybrid Homo sapiens
41 DDX23 9416
Co-fractionation Homo sapiens
42 CSTF3 1479
Affinity Capture-MS Homo sapiens
43 EIF4E 1977
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 CPSF4L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
46 CSTF2 1478
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 POLR2A 5430
Reconstituted Complex Homo sapiens
48 TUT1  
Affinity Capture-Western Homo sapiens
49 LRRC41 10489
Affinity Capture-MS Homo sapiens
50 NXF1 10482
Affinity Capture-RNA Homo sapiens
51 TBP  
Affinity Capture-RNA Homo sapiens
52 UFL1 23376
Affinity Capture-MS Homo sapiens
53 CPSF2 53981
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 KRAS 3845
Negative Genetic Homo sapiens
55 RNPS1 10921
Affinity Capture-MS Homo sapiens
56 MYC  
Affinity Capture-MS Homo sapiens
57 CSNK1A1 1452
Co-fractionation Homo sapiens
58 BARD1 580
Affinity Capture-MS Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 P4HB 5034
Affinity Capture-MS Homo sapiens
61 SPRTN  
Affinity Capture-MS Homo sapiens
62 ISY1 57461
Co-fractionation Homo sapiens
63 CPSF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RBM14 10432
Affinity Capture-MS Homo sapiens
65 FIP1L1 81608
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FBXW7  
Affinity Capture-MS Homo sapiens
67 IMPDH2 3615
Proximity Label-MS Homo sapiens
68 ARRDC3 57561
Affinity Capture-MS Homo sapiens
69 SNW1 22938
Affinity Capture-MS Homo sapiens
70 NPM1 4869
Affinity Capture-Western Homo sapiens
71 MDC1  
Affinity Capture-MS Homo sapiens
72 CDC5L 988
Affinity Capture-MS Homo sapiens
73 LMO1  
Affinity Capture-MS Homo sapiens
74 Tagap  
Affinity Capture-MS Mus musculus
75 POLR1E  
Co-fractionation Homo sapiens
76 PLD6  
Affinity Capture-MS Homo sapiens
77 ABCE1 6059
Affinity Capture-MS Homo sapiens
78 CPSF6 11052
Affinity Capture-MS Homo sapiens
79 PKN3 29941
Affinity Capture-MS Homo sapiens
80 SLBP  
Affinity Capture-Western Homo sapiens
81 DDRGK1 65992
Affinity Capture-MS Homo sapiens
82 UBE3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 EP300 2033
Affinity Capture-MS Homo sapiens
84 ISY1-RAB43 100534599
Co-fractionation Homo sapiens
85 RPA3 6119
Proximity Label-MS Homo sapiens
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