Gene description for DHX16
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 16
Gene symbol DHX16
Other names/aliases DBP2
DDX16
PRO2014
PRP8
PRPF2
Prp2
Species Homo sapiens
 Database cross references - DHX16
ExoCarta ExoCarta_8449
Vesiclepedia VP_8449
Entrez Gene 8449
HGNC 2739
MIM 603405
UniProt O60231  
 DHX16 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DHX16
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA helicase activity GO:0003724 TAS
    helicase activity GO:0004386 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    pattern recognition receptor activity GO:0038187 IDA
    ubiquitin binding GO:0043130 IDA
    molecular adaptor activity GO:0060090 IDA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 IMP
    mRNA splicing, via spliceosome GO:0000398 TAS
    chromatin remodeling GO:0006338 IEA
    RNA splicing GO:0008380 TAS
    antiviral innate immune response GO:0140374 IDA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IDA
    cytoplasm GO:0005737 IDA
    U2-type precatalytic spliceosome GO:0071005 IDA
 Experiment description of studies that identified DHX16 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DHX16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 DGCR14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 COIL  
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
6 PRKCD 5580
Affinity Capture-MS Homo sapiens
7 PCF11 51585
Co-fractionation Homo sapiens
8 LGALS9C  
Affinity Capture-MS Homo sapiens
9 NAA40  
Co-fractionation Homo sapiens
10 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
11 ACTC1 70
Proximity Label-MS Homo sapiens
12 NOP56 10528
Proximity Label-MS Homo sapiens
13 DHX8 1659
Proximity Label-MS Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 UBC 7316
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 CSNK2A1 1457
Biochemical Activity Homo sapiens
17 APP 351
Reconstituted Complex Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
Proximity Label-MS Homo sapiens
19 POLH  
Affinity Capture-MS Homo sapiens
20 CAPZB 832
Affinity Capture-MS Homo sapiens
21 DDX23 9416
Proximity Label-MS Homo sapiens
22 CPLX2 10814
Affinity Capture-MS Homo sapiens
23 RNPS1 10921
Affinity Capture-MS Homo sapiens
24 KIF23 9493
Affinity Capture-MS Homo sapiens
25 UBE2O 63893
Affinity Capture-MS Homo sapiens
26 AKIRIN2  
Affinity Capture-MS Homo sapiens
27 FBXW7  
Affinity Capture-MS Homo sapiens
28 CPSF7 79869
Co-fractionation Homo sapiens
29 SRRM2 23524
Affinity Capture-MS Homo sapiens
30 SNRPC 6631
Affinity Capture-MS Homo sapiens
31 OIP5  
Affinity Capture-MS Homo sapiens
32 OBSL1 23363
Affinity Capture-MS Homo sapiens
33 DDX58 23586
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
34 GSPT1 2935
Co-fractionation Homo sapiens
35 SLX4  
Affinity Capture-MS Homo sapiens
36 FBXO5  
Affinity Capture-MS Homo sapiens
37 NUDCD1 84955
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
38 SERF2 10169
Affinity Capture-MS Homo sapiens
39 MECP2 4204
Affinity Capture-MS Homo sapiens
40 MAVS 57506
Affinity Capture-Western Homo sapiens
41 Bud13  
Affinity Capture-MS Mus musculus
42 TFIP11  
Two-hybrid Homo sapiens
43 FADS3 3995
Affinity Capture-MS Homo sapiens
44 Gpkow  
Affinity Capture-MS Mus musculus
45 LSM2 57819
Co-fractionation Homo sapiens
46 AP3M1 26985
Affinity Capture-MS Homo sapiens
47 POLD1 5424
Affinity Capture-MS Homo sapiens
48 ERCC3  
Co-fractionation Homo sapiens
49 MRE11A 4361
Co-fractionation Homo sapiens
50 FOXN2  
Affinity Capture-MS Homo sapiens
51 PARK2  
Affinity Capture-MS Homo sapiens
52 SNRNP40 9410
Protein-RNA Homo sapiens
53 TCEA2  
Co-fractionation Homo sapiens
54 SRSF6 6431
Affinity Capture-MS Homo sapiens
55 IFI27L1  
Affinity Capture-MS Homo sapiens
56 CBX3 11335
Proximity Label-MS Homo sapiens
57 GCC2 9648
Co-fractionation Homo sapiens
58 AKR7L 246181
Affinity Capture-MS Homo sapiens
59 FOXL2  
Affinity Capture-MS Homo sapiens
60 NTRK1 4914
Affinity Capture-MS Homo sapiens
61 RPL31 6160
Affinity Capture-MS Homo sapiens
62 U2AF2 11338
Co-fractionation Homo sapiens
63 CSRP2BP  
Affinity Capture-MS Homo sapiens
64 CSNK1E 1454
Co-fractionation Homo sapiens
65 SNRNP27  
Protein-RNA Homo sapiens
66 RBBP8  
Affinity Capture-MS Homo sapiens
67 C1orf189  
Affinity Capture-MS Homo sapiens
68 CD3EAP  
Proximity Label-MS Homo sapiens
69 MTR 4548
Co-fractionation Homo sapiens
70 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SF3B2 10992
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
72 MIF4GD 57409
Co-fractionation Homo sapiens
73 PYROXD1 79912
Co-fractionation Homo sapiens
74 TCEAL7  
Affinity Capture-MS Homo sapiens
75 XPO1 7514
Affinity Capture-MS Homo sapiens
76 HELQ  
Co-fractionation Homo sapiens
77 PSENEN  
Affinity Capture-MS Homo sapiens
78 SIRT7  
Affinity Capture-MS Homo sapiens
79 Smad3  
Affinity Capture-MS Mus musculus
80 TXLNB  
Affinity Capture-MS Homo sapiens
81 TJP1 7082
Co-fractionation Homo sapiens
82 XAB2 56949
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
83 EGFR 1956
Affinity Capture-MS Homo sapiens
84 MAB21L2  
Affinity Capture-MS Homo sapiens
85 CDKL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 TRIM6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
87 FTH1 2495
Affinity Capture-MS Homo sapiens
88 MYO19  
Two-hybrid Homo sapiens
89 PUS7 54517
Co-fractionation Homo sapiens
90 LZTS2 84445
Affinity Capture-MS Homo sapiens
91 LBH  
Affinity Capture-MS Homo sapiens
92 TADA2A  
Affinity Capture-MS Homo sapiens
93 RPA3 6119
Proximity Label-MS Homo sapiens
94 TOP1 7150
Affinity Capture-MS Homo sapiens
95 BLMH 642
Affinity Capture-MS Homo sapiens
96 GABPB1  
Co-fractionation Homo sapiens
97 SETDB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 DDRGK1 65992
Affinity Capture-MS Homo sapiens
99 WDR44 54521
Co-fractionation Homo sapiens
100 CDC20B  
Affinity Capture-MS Homo sapiens
101 BIRC8  
Affinity Capture-MS Homo sapiens
102 SUMO1 7341
Affinity Capture-MS Homo sapiens
103 NUDT21 11051
Co-fractionation Homo sapiens
104 PRMT3 10196
Affinity Capture-MS Homo sapiens
105 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
106 PARP1 142
Proximity Label-MS Homo sapiens
107 ALKBH2  
Co-fractionation Homo sapiens
108 ILF3 3609
Co-fractionation Homo sapiens
109 SRRM1 10250
Affinity Capture-MS Homo sapiens
110 TP53 7157
Affinity Capture-MS Homo sapiens
111 HOMEZ  
Two-hybrid Homo sapiens
112 ZFYVE27 118813
Affinity Capture-MS Homo sapiens
113 AJUBA  
Affinity Capture-MS Homo sapiens
114 PTBP1 5725
Co-fractionation Homo sapiens
115 EHD2 30846
Co-fractionation Homo sapiens
116 GON4L  
Co-fractionation Homo sapiens
117 CAPRIN1 4076
Co-fractionation Homo sapiens
118 LSM5  
Co-fractionation Homo sapiens
119 EZH1  
Affinity Capture-MS Homo sapiens
120 MEGF10 84466
Two-hybrid Homo sapiens
121 DHX40  
Proximity Label-MS Homo sapiens
122 C20orf197  
Affinity Capture-MS Homo sapiens
123 JAK3 3718
Affinity Capture-MS Homo sapiens
124 SLC2A7  
Affinity Capture-MS Homo sapiens
125 NUP50 10762
Proximity Label-MS Homo sapiens
126 Srsf1 110809
Affinity Capture-MS Mus musculus
127 C2orf73  
Affinity Capture-MS Homo sapiens
128 SPCS1 28972
Affinity Capture-MS Homo sapiens
129 DHX38 9785
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
130 PINK1  
Affinity Capture-MS Homo sapiens
131 TTC29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 SERBP1 26135
Affinity Capture-MS Homo sapiens
133 LSM6 11157
Co-fractionation Homo sapiens
134 CUL7 9820
Affinity Capture-MS Homo sapiens
135 KRAS 3845
Negative Genetic Homo sapiens
136 CPSF6 11052
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
137 GPKOW  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
138 CPSF3 51692
Co-fractionation Homo sapiens
139 EP300 2033
Affinity Capture-MS Homo sapiens
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