Gene description for LSM2
Gene name LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Gene symbol LSM2
Other names/aliases C6orf28
G7B
YBL026W
snRNP
Species Homo sapiens
 Database cross references - LSM2
ExoCarta ExoCarta_57819
Vesiclepedia VP_57819
Entrez Gene 57819
HGNC 13940
MIM 607282
UniProt Q9Y333  
 LSM2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for LSM2
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
    U6 snRNA binding GO:0017070 NAS
    small GTPase binding GO:0031267 IPI
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 IDA
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
    mRNA catabolic process GO:0006402 IDA
Subcellular Localization
    P-body GO:0000932 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    U6 snRNP GO:0005688 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IPI
    U2-type precatalytic spliceosome GO:0071005 IDA
    precatalytic spliceosome GO:0071011 IBA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
    Lsm2-8 complex GO:0120115 IDA
    Lsm1-7-Pat1 complex GO:1990726 IBA
 Experiment description of studies that identified LSM2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LSM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
3 MAGEA3  
Two-hybrid Homo sapiens
4 Lsm8  
Two-hybrid Mus musculus
5 AES 166
Two-hybrid Homo sapiens
6 REL 5966
Two-hybrid Homo sapiens
7 LGALS7B 653499
Two-hybrid Homo sapiens
8 LSM4 25804
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
9 SSB 6741
Co-fractionation Homo sapiens
10 PRPF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CLTB 1212
Affinity Capture-MS Homo sapiens
12 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SNRPD2 6633
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 TRIM54  
Two-hybrid Homo sapiens
15 ZMAT2  
Co-fractionation Homo sapiens
16 RNH1 6050
Co-fractionation Homo sapiens
17 CAPZB 832
Affinity Capture-MS Homo sapiens
18 Lsm1  
Two-hybrid Mus musculus
19 CORO1B 57175
Co-fractionation Homo sapiens
20 ZBTB16  
Two-hybrid Homo sapiens
21 ZNF408  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 KIF5B 3799
Co-fractionation Homo sapiens
23 SNRPC 6631
Affinity Capture-MS Homo sapiens
24 CLNS1A 1207
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 TRIM56 81844
Co-fractionation Homo sapiens
26 SNRPD3 6634
Co-fractionation Homo sapiens
27 PRCC  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 PKN2 5586
Co-fractionation Homo sapiens
29 RBM42  
Affinity Capture-MS Homo sapiens
30 BUD31 8896
Co-fractionation Homo sapiens
31 LSM1 27257
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
32 RBM8A 9939
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
33 LSM12 124801
Two-hybrid Homo sapiens
34 RAB1B 81876
Co-fractionation Homo sapiens
35 YWHAB 7529
Co-fractionation Homo sapiens
36 BANP  
Two-hybrid Homo sapiens
37 LMNB1 4001
Co-fractionation Homo sapiens
38 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
39 DDX21 9188
Affinity Capture-MS Homo sapiens
40 EMD 2010
Affinity Capture-MS Homo sapiens
41 MEPCE 56257
Affinity Capture-MS Homo sapiens
42 RBM6 10180
Affinity Capture-MS Homo sapiens
43 RALA 5898
Two-hybrid Homo sapiens
44 RSRC1  
Two-hybrid Homo sapiens
45 DDX20 11218
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
46 SNRPD1 6632
Co-fractionation Homo sapiens
47 DNAJA1 3301
Two-hybrid Homo sapiens
48 MEOX2  
Two-hybrid Homo sapiens
49 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CRMP1 1400
Two-hybrid Homo sapiens
51 CEBPA  
Protein-peptide Homo sapiens
52 SNX1 6642
Co-fractionation Homo sapiens
53 SF3B2 10992
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 RBM39 9584
Affinity Capture-MS Homo sapiens
55 LSM8 51691
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
56 USP4 7375
Affinity Capture-MS Homo sapiens
57 NAA38  
Co-fractionation Homo sapiens
58 C14orf1 11161
Two-hybrid Homo sapiens
59 PYROXD1 79912
Co-fractionation Homo sapiens
60 USP15 9958
Affinity Capture-MS Homo sapiens
61 SNRPB 6628
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
62 LGALS7 3963
Two-hybrid Homo sapiens
63 RNU4ATAC  
Affinity Capture-Western Homo sapiens
64 TXNDC9 10190
Co-fractionation Homo sapiens
65 SF3A1 10291
Affinity Capture-MS Homo sapiens
66 PATL1 219988
Two-hybrid Homo sapiens
67 DKC1 1736
Co-fractionation Homo sapiens
68 PSPC1 55269
Affinity Capture-MS Homo sapiens
69 LSM3 27258
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
70 TOP1 7150
Affinity Capture-MS Homo sapiens
71 PRPF4 9128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 SART3 9733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 GBP2  
Two-hybrid Homo sapiens
74 DDB1 1642
Co-fractionation Homo sapiens
75 MAGEA6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
76 LRRC6  
Two-hybrid Homo sapiens
77 PCM1 5108
Two-hybrid Homo sapiens
78 EXOSC10 5394
Two-hybrid Homo sapiens
79 SMN1 6606
Reconstituted Complex Homo sapiens
80 ILF3 3609
Co-fractionation Homo sapiens
81 ZRANB2 9406
Co-fractionation Homo sapiens
82 CPSF6 11052
Affinity Capture-MS Homo sapiens
83 RNU6ATAC  
Affinity Capture-Western Homo sapiens
84 CWC27 10283
Co-fractionation Homo sapiens
85 SNRPA1 6627
Co-fractionation Homo sapiens
86 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
87 SNRPA 6626
Affinity Capture-MS Homo sapiens
88 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
89 LSM5  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
90 MKI67  
Affinity Capture-MS Homo sapiens
91 LSM7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
92 DHX16 8449
Co-fractionation Homo sapiens
93 DHX38 9785
Co-fractionation Homo sapiens
94 CBX8 57332
Two-hybrid Homo sapiens
95 CLTA 1211
Affinity Capture-MS Homo sapiens
96 CIT 11113
Affinity Capture-MS Homo sapiens
97 SERBP1 26135
Affinity Capture-MS Homo sapiens
98 LSM6 11157
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
99 KRT13 3860
Two-hybrid Homo sapiens
100 KRAS 3845
Negative Genetic Homo sapiens
101 Srsf3 20383
Two-hybrid Mus musculus
102 SSRP1 6749
Affinity Capture-MS Homo sapiens
103 LNX1  
Two-hybrid Homo sapiens
104 KDM5C  
Co-fractionation Homo sapiens
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