Gene description for CBS
Gene name cystathionine-beta-synthase
Gene symbol CBS
Other names/aliases HIP4
Species Homo sapiens
 Database cross references - CBS
ExoCarta ExoCarta_875
Vesiclepedia VP_875
Entrez Gene 875
HGNC 1550
MIM 613381
UniProt P35520  
 CBS identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CBS
Molecular Function
    cystathionine beta-synthase activity GO:0004122 IBA
    cystathionine beta-synthase activity GO:0004122 IDA
    protein binding GO:0005515 IPI
    oxygen binding GO:0019825 IDA
    enzyme binding GO:0019899 IPI
    heme binding GO:0020037 EXP
    heme binding GO:0020037 IDA
    heme binding GO:0020037 IMP
    pyridoxal phosphate binding GO:0030170 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    nitrite reductase (NO-forming) activity GO:0050421 IDA
    carbon monoxide binding GO:0070025 IDA
    nitric oxide binding GO:0070026 IDA
    modified amino acid binding GO:0072341 IDA
    S-adenosyl-L-methionine binding GO:1904047 IDA
Biological Process
    endochondral ossification GO:0001958 IEA
    blood vessel remodeling GO:0001974 IEA
    cysteine biosynthetic process from serine GO:0006535 IBA
    L-serine metabolic process GO:0006563 IDA
    L-serine catabolic process GO:0006565 IDA
    superoxide metabolic process GO:0006801 IEA
    regulation of nitric oxide mediated signal transduction GO:0010749 IEA
    cysteine biosynthetic process via cystathionine GO:0019343 IEA
    cysteine biosynthetic process GO:0019344 IDA
    transsulfuration GO:0019346 IEA
    L-cysteine catabolic process GO:0019448 IDA
    cerebellum morphogenesis GO:0021587 IEA
    DNA protection GO:0042262 IMP
    negative regulation of apoptotic process GO:0043066 IEA
    homocysteine catabolic process GO:0043418 IDA
    homocysteine metabolic process GO:0050667 IDA
    response to folic acid GO:0051593 IEA
    maternal process involved in female pregnancy GO:0060135 IEA
    cartilage development involved in endochondral bone morphogenesis GO:0060351 IEA
    hydrogen sulfide biosynthetic process GO:0070814 IDA
    hydrogen sulfide biosynthetic process GO:0070814 ISS
    cellular response to hypoxia GO:0071456 IEA
    blood vessel diameter maintenance GO:0097746 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 HDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CBS in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CBS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 NPLOC4 55666
Co-fractionation Homo sapiens
4 QARS 5859
Co-fractionation Homo sapiens
5 SKIV2L 6499
Co-fractionation Homo sapiens
6 PKM 5315
Co-fractionation Homo sapiens
7 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 C14orf142  
Co-fractionation Homo sapiens
10 ARHGAP11B  
Affinity Capture-MS Homo sapiens
11 UBC 7316
Affinity Capture-MS Homo sapiens
12 YTHDF2 51441
Affinity Capture-RNA Homo sapiens
13 NEK7 140609
Affinity Capture-MS Homo sapiens
14 KIF23 9493
Affinity Capture-MS Homo sapiens
15 COL4A3BP 10087
Two-hybrid Homo sapiens
16 HGS 9146
Reconstituted Complex Homo sapiens
17 MSX2  
Affinity Capture-MS Homo sapiens
18 PTPRR  
Affinity Capture-MS Homo sapiens
19 SRP14 6727
Co-fractionation Homo sapiens
20 LRSAM1 90678
Co-fractionation Homo sapiens
21 GOPC 57120
Co-fractionation Homo sapiens
22 GPN1  
Co-fractionation Homo sapiens
23 OAT 4942
Co-fractionation Homo sapiens
24 EEF1A1 1915
Co-fractionation Homo sapiens
25 UGP2 7360
Two-hybrid Homo sapiens
26 ENOPH1 58478
Co-fractionation Homo sapiens
27 CTH 1491
Co-fractionation Homo sapiens
28 ABAT  
Co-fractionation Homo sapiens
29 ZDHHC17 23390
Two-hybrid Homo sapiens
30 RAD23B 5887
Co-fractionation Homo sapiens
31 DBNL 28988
Co-fractionation Homo sapiens
32 ERO1L 30001
Co-fractionation Homo sapiens
33 RTFDC1  
Co-fractionation Homo sapiens
34 ZFYVE16  
Proximity Label-MS Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 DERA 51071
Co-fractionation Homo sapiens
37 PRKAG1 5571
Two-hybrid Homo sapiens
38 UBE2I 7329
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 PARP6  
Co-fractionation Homo sapiens
41 AP1B1 162
Co-fractionation Homo sapiens
42 IARS 3376
Co-fractionation Homo sapiens
43 RSU1 6251
Co-fractionation Homo sapiens
44 DAXX  
Two-hybrid Homo sapiens
45 CARS 833
Co-fractionation Homo sapiens
46 KDF1  
Affinity Capture-MS Homo sapiens
47 CBX4  
Two-hybrid Homo sapiens
48 UBE2N 7334
Affinity Capture-MS Homo sapiens
49 ARHGAP32  
Affinity Capture-MS Homo sapiens
50 PREX1  
Affinity Capture-MS Homo sapiens
51 PRKAR1A 5573
Two-hybrid Homo sapiens
52 CUL3 8452
Affinity Capture-MS Homo sapiens
53 PIAS3  
Two-hybrid Homo sapiens
54 VBP1 7411
Co-fractionation Homo sapiens
55 RANBP9 10048
Two-hybrid Homo sapiens
56 CDK2 1017
Affinity Capture-MS Homo sapiens
57 RPA3 6119
Proximity Label-MS Homo sapiens
58 UBA2 10054
Co-fractionation Homo sapiens
59 PIAS1 8554
Two-hybrid Homo sapiens
60 UBASH3A 53347
Two-hybrid Homo sapiens
61 FXR2 9513
Two-hybrid Homo sapiens
62 NUCB1 4924
Co-fractionation Homo sapiens
63 PLIN3 10226
Co-fractionation Homo sapiens
64 NMD3 51068
Co-fractionation Homo sapiens
65 PA2G4 5036
Affinity Capture-MS Homo sapiens
66 SEC24B 10427
Co-fractionation Homo sapiens
67 HSPE1 3336
Co-fractionation Homo sapiens
68 UBE3B 89910
Co-fractionation Homo sapiens
69 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
70 HID1 283987
Two-hybrid Homo sapiens
71 FBXL6  
Affinity Capture-MS Homo sapiens
72 UBA5 79876
Co-fractionation Homo sapiens
73 IREB2 3658
Co-fractionation Homo sapiens
74 ASNS 440
Co-fractionation Homo sapiens
75 PIN1 5300
Two-hybrid Homo sapiens
76 EHHADH 1962
Two-hybrid Homo sapiens
77 MEN1 4221
Affinity Capture-MS Homo sapiens
78 ZMYM5 9205
Two-hybrid Homo sapiens
79 VTA1 51534
Two-hybrid Homo sapiens
80 OTUB1 55611
Affinity Capture-MS Homo sapiens
81 PSMA1 5682
Two-hybrid Homo sapiens
82 TRAP1 10131
Affinity Capture-MS Homo sapiens
83 PDLIM5 10611
Co-fractionation Homo sapiens
84 IPO4 79711
Co-fractionation Homo sapiens
85 HSF1  
Co-fractionation Homo sapiens
86 NF2 4771
Affinity Capture-MS Homo sapiens
87 ARHGAP29 9411
Affinity Capture-MS Homo sapiens
88 SERBP1 26135
Affinity Capture-MS Homo sapiens
89 C9orf72  
Affinity Capture-MS Homo sapiens
90 ARHGAP35 2909
Affinity Capture-MS Homo sapiens
91 BAG1 573
Affinity Capture-MS Homo sapiens
92 SEC31A 22872
Co-fractionation Homo sapiens
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