Gene description for HGS
Gene name hepatocyte growth factor-regulated tyrosine kinase substrate
Gene symbol HGS
Other names/aliases HRS
Species Homo sapiens
 Database cross references - HGS
ExoCarta ExoCarta_9146
Vesiclepedia VP_9146
Entrez Gene 9146
HGNC 4897
MIM 604375
UniProt O14964  
 HGS identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 30089911    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Lung cancer cells 30089911    
Lung cancer cells 30089911    
Lung cancer cells 30089911    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
T lymphocytes 34108659    
Urine 19056867    
 Gene ontology annotations for HGS
Molecular Function
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    phosphatidylinositol binding GO:0035091 IEA
    ubiquitin binding GO:0043130 IBA
    ubiquitin-like protein ligase binding GO:0044389 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    protein targeting to lysosome GO:0006622 IMP
    signal transduction GO:0007165 TAS
    negative regulation of cell population proliferation GO:0008285 TAS
    membrane invagination GO:0010324 IMP
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of platelet-derived growth factor receptor signaling pathway GO:0010642 IMP
    endosomal transport GO:0016197 NAS
    macroautophagy GO:0016236 TAS
    negative regulation of angiogenesis GO:0016525 IMP
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 IMP
    receptor internalization GO:0031623 IBA
    endocytic recycling GO:0032456 IBA
    multivesicular body assembly GO:0036258 NAS
    multivesicular body assembly GO:0036258 TAS
    regulation of protein catabolic process GO:0042176 TAS
    protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043328 NAS
    regulation of MAP kinase activity GO:0043405 IMP
    negative regulation of receptor signaling pathway via JAK-STAT GO:0046426 IDA
    protein localization to membrane GO:0072657 IMP
    membrane fission GO:0090148 NAS
    positive regulation of exosomal secretion GO:1903543 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    endosome GO:0005768 IDA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    early endosome membrane GO:0031901 IEA
    multivesicular body membrane GO:0032585 IEA
    ESCRT-0 complex GO:0033565 IDA
    ESCRT-0 complex GO:0033565 IPI
    ESCRT-0 complex GO:0033565 ISS
    ESCRT-0 complex GO:0033565 TAS
    extracellular exosome GO:0070062 HDA
    phagocytic vesicle lumen GO:0097013 TAS
 Experiment description of studies that identified HGS in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 989
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
17
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
18
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
30
Experiment ID 986
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 987
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H358
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 988
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H1264
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
33
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
34
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 955
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name A375
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
36
Experiment ID 956
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name UACC-903
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
37
Experiment ID 957
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
Immuno-EM
ELISA
38
Experiment ID 958
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM164
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
ELISA
39
Experiment ID 963
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9 - Fraction 6
Isolation/purification methods Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation
Flotation density 1.131 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
40
Experiment ID 964
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9 - Fraction 7
Isolation/purification methods Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation
Flotation density 1.144 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
41
Experiment ID 965
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9 - Fraction 8
Isolation/purification methods Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation
Flotation density 1.156 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
42
Experiment ID 966
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9 - Fraction 9
Isolation/purification methods Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation
Flotation density 1.178 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
43
Experiment ID 967
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9 - Fraction 10
Isolation/purification methods Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation
Flotation density 1.193 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
44
Experiment ID 971
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
45
Experiment ID 972
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
46
Experiment ID 974
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
47
Experiment ID 984
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name MEL624
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
48
Experiment ID 985
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name MEL624
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Reverse phase protein array
Western blotting
ELISA
49
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
50
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
51
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
52
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
53
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
54
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
55
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
56
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
57
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
58
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
59
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
60
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
61
Experiment ID 977
MISEV standards
✘ Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Plasma
Sample name Healthy donors
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
ELISA
62
Experiment ID 978
MISEV standards
✘ Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Plasma
Sample name Melanoma stage IV patients
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
ELISA
63
Experiment ID 979
MISEV standards
✘ Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Plasma
Sample name Melanoma stage IV patients - Fraction 7
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density 1.44 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
64
Experiment ID 980
MISEV standards
✘ Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Plasma
Sample name Melanoma stage IV patients - Fraction 8
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density 1.56 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
65
Experiment ID 981
MISEV standards
✘ Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Plasma
Sample name Melanoma stage IV patients - Fraction 9
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density 1.178 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
66
Experiment ID 982
MISEV standards
✘ Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Plasma
Sample name Melanoma stage IV patients - Fraction 10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density 1.193 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
67
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
68
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
69
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
70
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
71
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
72
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
73
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
74
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
75
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HGS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUSC2  
Affinity Capture-MS Homo sapiens
2 KRT37 8688
Two-hybrid Homo sapiens
3 NRSN1  
Affinity Capture-MS Homo sapiens
4 AES 166
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
5 UBA1 7317
Reconstituted Complex Homo sapiens
6 VMP1 81671
Reconstituted Complex Homo sapiens
7 ABCA1 19
Affinity Capture-Western Homo sapiens
8 PLCD1 5333
Two-hybrid Homo sapiens
9 GJD3  
Proximity Label-MS Homo sapiens
10 UBC 7316
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 CSNK2A1 1457
Biochemical Activity Homo sapiens
12 KRT82 3888
Two-hybrid Homo sapiens
13 ATN1  
Two-hybrid Homo sapiens
14 DECR1 1666
Reconstituted Complex Homo sapiens
15 ARMC7  
Two-hybrid Homo sapiens
16 NDC80 10403
Two-hybrid Homo sapiens
17 BLOC1S1  
Two-hybrid Homo sapiens
18 PRR22  
Two-hybrid Homo sapiens
19 SAPCD1  
Two-hybrid Homo sapiens
20 ESRRG  
Reconstituted Complex Homo sapiens
21 TIMM10B 26515
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 OPRM1 4988
Co-localization Homo sapiens
23 HSPH1 10808
Affinity Capture-MS Homo sapiens
24 OIP5  
Two-hybrid Homo sapiens
25 KRT31 3881
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
26 RGS9BP  
Affinity Capture-MS Homo sapiens
27 VPS4A 27183
Affinity Capture-Western Homo sapiens
28 SMAD5 4090
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
29 TFRC 7037
Affinity Capture-Western Homo sapiens
30 STX12 23673
Affinity Capture-Western Homo sapiens
31 GFI1B  
Two-hybrid Homo sapiens
32 RNF11 26994
Affinity Capture-Western Homo sapiens
33 MEST 4232
Reconstituted Complex Homo sapiens
34 UBQLNL  
Two-hybrid Homo sapiens
35 PMEPA1 56937
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 UBE4B 10277
Reconstituted Complex Homo sapiens
37 MED7  
Two-hybrid Homo sapiens
38 KRT39  
Two-hybrid Homo sapiens
39 CSTF2 1478
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 KRT6A 3853
Two-hybrid Homo sapiens
41 FOXD4L1  
Two-hybrid Homo sapiens
42 PLEKHB2 55041
Two-hybrid Homo sapiens
43 SYNJ2BP 55333
Co-fractionation Homo sapiens
44 ACTN4 81
Co-fractionation Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
45 FLOT1 10211
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
46 MED25  
Two-hybrid Homo sapiens
47 LYST 1130
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
48 FLOT2 2319
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
49 MVB12A 93343
Affinity Capture-MS Homo sapiens
50 PKNOX2  
Two-hybrid Homo sapiens
51 VPS37B 79720
Two-hybrid Homo sapiens
52 STAM 8027
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
53 DSN1 79980
Two-hybrid Homo sapiens
54 BBC3  
Two-hybrid Homo sapiens
55 RDH14 57665
Affinity Capture-MS Homo sapiens
56 CEP63  
Two-hybrid Homo sapiens
57 AHCYL1 10768
Reconstituted Complex Homo sapiens
58 ANKRD55  
Two-hybrid Homo sapiens
59 KRT86  
Two-hybrid Homo sapiens
60 KRT35 3886
Two-hybrid Homo sapiens
61 TMEM189  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
62 UBQLN2 29978
Two-hybrid Homo sapiens
63 MAGEB4  
Two-hybrid Homo sapiens
64 GOLT1B 51026
Affinity Capture-MS Homo sapiens
65 CUL3 8452
Affinity Capture-MS Homo sapiens
66 EGFL7 51162
Two-hybrid Homo sapiens
67 DLG4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
68 KRT26 353288
Two-hybrid Homo sapiens
69 NPAS2  
Two-hybrid Homo sapiens
70 KRT27 342574
Two-hybrid Homo sapiens
71 KRT15 3866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 IST1 9798
Affinity Capture-MS Homo sapiens
73 TJP2 9414
Reconstituted Complex Homo sapiens
74 ING5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 HSPA1A 3303
Affinity Capture-MS Homo sapiens
76 TCP11L2  
Two-hybrid Homo sapiens
77 SORBS3 10174
Two-hybrid Homo sapiens
78 P4HA3 283208
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
79 KRT19 3880
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
80 MAP3K10  
Reconstituted Complex Homo sapiens
81 TBX19  
Two-hybrid Homo sapiens
82 SPIRE2  
Two-hybrid Homo sapiens
83 YBX1 4904
Cross-Linking-MS (XL-MS) Homo sapiens
84 BCAS2 10286
Two-hybrid Homo sapiens
85 DTX3L 151636
Affinity Capture-Western Homo sapiens
86 GRB2 2885
Affinity Capture-MS Homo sapiens
87 WDR77 79084
Co-fractionation Homo sapiens
88 RHOBTB3 22836
Reconstituted Complex Homo sapiens
89 ZNF430  
Two-hybrid Homo sapiens
90 JMJD7  
Two-hybrid Homo sapiens
91 CDSN 1041
Two-hybrid Homo sapiens
92 DCTN2 10540
Reconstituted Complex Homo sapiens
93 G3BP2 9908
Affinity Capture-MS Homo sapiens
94 ARFIP2 23647
Two-hybrid Homo sapiens
95 IL4R  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
96 WWP2 11060
Biochemical Activity Homo sapiens
97 KRT24 192666
Two-hybrid Homo sapiens
98 PSMA5 5686
Affinity Capture-MS Homo sapiens
99 JAKMIP2  
Two-hybrid Homo sapiens
100 CLTA 1211
Proximity Label-MS Homo sapiens
101 TRIM69 140691
Two-hybrid Homo sapiens
102 EPHA2 1969
Proximity Label-MS Homo sapiens
103 PPP2R3B  
Two-hybrid Homo sapiens
104 KRT13 3860
Two-hybrid Homo sapiens
105 SCAMP3 10067
Affinity Capture-Western Homo sapiens
106 REPIN1  
Co-fractionation Homo sapiens
107 LILRA5  
Affinity Capture-MS Homo sapiens
108 TRIM3 10612
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
109 CCDC103  
Two-hybrid Homo sapiens
110 METTL7A 25840
Proximity Label-MS Homo sapiens
111 GJA1 2697
Co-localization Homo sapiens
Proximity Label-MS Homo sapiens
112 POGZ 23126
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
113 NUP62 23636
Two-hybrid Homo sapiens
114 ADRB2  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
115 APC  
Two-hybrid Homo sapiens
116 GFAP 2670
Reconstituted Complex Homo sapiens
117 NTNG1  
Affinity Capture-MS Homo sapiens
118 USP16  
Co-fractionation Homo sapiens
119 VAMP2 6844
Affinity Capture-Western Homo sapiens
120 KRTAP7-1  
Two-hybrid Homo sapiens
121 FIGN  
Two-hybrid Homo sapiens
122 FTCD 10841
Two-hybrid Homo sapiens
123 HSPA2 3306
Affinity Capture-MS Homo sapiens
124 GKAP1  
Two-hybrid Homo sapiens
125 SYK 6850
Biochemical Activity Homo sapiens
126 WASH1 100287171
Co-localization Homo sapiens
Two-hybrid Homo sapiens
127 SUN2 25777
Reconstituted Complex Homo sapiens
128 ZNF302  
Reconstituted Complex Homo sapiens
129 KLF4  
Two-hybrid Homo sapiens
130 HAP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
131 FSD2 123722
Two-hybrid Homo sapiens
132 EXOC7 23265
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
133 EBAG9 9166
Proximity Label-MS Homo sapiens
134 RSPO4  
Two-hybrid Homo sapiens
135 PRNP 5621
Affinity Capture-MS Homo sapiens
136 YWHAB 7529
Affinity Capture-MS Homo sapiens
137 RUNX1  
Two-hybrid Homo sapiens
138 PFKM 5213
Reconstituted Complex Homo sapiens
139 STAM2 10254
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
140 BSG 682
Reconstituted Complex Homo sapiens
141 MIB1 57534
Proximity Label-MS Homo sapiens
142 TEKT5  
Two-hybrid Homo sapiens
143 TTC4  
Co-fractionation Homo sapiens
144 CEP55 55165
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
145 NUTM2F  
Two-hybrid Homo sapiens
146 PPP1R16A 84988
Reconstituted Complex Homo sapiens
147 KRT14 3861
Two-hybrid Homo sapiens
148 DYDC1  
Two-hybrid Homo sapiens
149 TRIM17  
Two-hybrid Homo sapiens
150 KIAA0753  
Two-hybrid Homo sapiens
151 BRINP3  
Reconstituted Complex Homo sapiens
152 TEKT1  
Two-hybrid Homo sapiens
153 LAPTM4B  
Affinity Capture-Western Homo sapiens
154 TUBB 203068
Reconstituted Complex Homo sapiens
155 MIF4GD 57409
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
156 ACLY 47
Reconstituted Complex Homo sapiens
157 KRT25 147183
Two-hybrid Homo sapiens
158 ILKAP 80895
Reconstituted Complex Homo sapiens
159 UBAP2 55833
Two-hybrid Homo sapiens
160 KIAA0825  
Two-hybrid Homo sapiens
161 RSU1 6251
Reconstituted Complex Homo sapiens
162 SNX7 51375
Two-hybrid Homo sapiens
163 USP54 159195
Two-hybrid Homo sapiens
164 EGFR 1956
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
165 TFG 10342
Two-hybrid Homo sapiens
166 TGM7  
Affinity Capture-MS Homo sapiens
167 SMAD1 4086
Affinity Capture-Western Homo sapiens
168 FGFR2 2263
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
169 VGLL3  
Two-hybrid Homo sapiens
170 TADA2A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
171 SDS  
Two-hybrid Homo sapiens
172 EHMT2 10919
Two-hybrid Homo sapiens
173 SNX12 29934
Co-fractionation Homo sapiens
174 MET 4233
Affinity Capture-Western Homo sapiens
175 IL2RB  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
176 RHOB 388
Proximity Label-MS Homo sapiens
177 AVPR2  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
178 KRT18 3875
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
179 RNF43  
Proximity Label-MS Homo sapiens
180 FBN1 2200
Two-hybrid Homo sapiens
181 EIF3F 8665
Two-hybrid Homo sapiens
182 SERGEF  
Two-hybrid Homo sapiens
183 RASSF4  
Two-hybrid Homo sapiens
184 SLK 9748
Co-fractionation Homo sapiens
185 NMI  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
186 MKNK1  
Two-hybrid Homo sapiens
187 C3orf36  
Two-hybrid Homo sapiens
188 SQSTM1 8878
Proximity Label-MS Homo sapiens
189 SMAD4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
190 SNAP25 6616
Affinity Capture-Western Homo sapiens
191 SHMT2 6472
Affinity Capture-RNA Homo sapiens
192 GOLGA7B  
Two-hybrid Homo sapiens
193 HNRNPK 3190
Co-fractionation Homo sapiens
194 MED21  
Two-hybrid Homo sapiens
195 ABI2 10152
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
196 PLD3 23646
Reconstituted Complex Homo sapiens
197 BCL2L1 598
Affinity Capture-MS Homo sapiens
198 KRT38 8687
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
199 YBX3 8531
Cross-Linking-MS (XL-MS) Homo sapiens
200 ATP1A1 476
Reconstituted Complex Homo sapiens
201 LDOC1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
202 GGA2 23062
Reconstituted Complex Homo sapiens
203 KIR2DL4  
Affinity Capture-MS Homo sapiens
204 KRAS 3845
Negative Genetic Homo sapiens
205 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
206 LMO1  
Two-hybrid Homo sapiens
207 VPS33B 26276
Affinity Capture-Western Homo sapiens
208 LURAP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
209 C17orf59 54785
Two-hybrid Homo sapiens
210 CCND3  
Two-hybrid Homo sapiens
211 CBS 875
Reconstituted Complex Homo sapiens
212 KRT76 51350
Two-hybrid Homo sapiens
213 OCLN 100506658
Affinity Capture-Western Homo sapiens
214 BEGAIN  
Two-hybrid Homo sapiens
215 CD4 920
Affinity Capture-Western Homo sapiens
216 EPS15 2060
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
217 ARL6IP1 23204
Reconstituted Complex Homo sapiens
218 TRIM54  
Two-hybrid Homo sapiens
219 ZNF44  
Two-hybrid Homo sapiens
220 MRFAP1L1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
221 FAM173A  
Two-hybrid Homo sapiens
222 CEP57L1  
Two-hybrid Homo sapiens
223 TIMMDC1  
Reconstituted Complex Homo sapiens
224 KRT40  
Two-hybrid Homo sapiens
225 PPP1R7 5510
Reconstituted Complex Homo sapiens
226 SF3B3 23450
Reconstituted Complex Homo sapiens
227 CRMP1 1400
Reconstituted Complex Homo sapiens
228 EDAR  
Affinity Capture-MS Homo sapiens
229 KRTAP19-5  
Two-hybrid Homo sapiens
230 GLTSCR1L  
Two-hybrid Homo sapiens
231 UBQLN4 56893
Two-hybrid Homo sapiens
232 HSPA8 3312
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
233 GOLGA6L9  
Two-hybrid Homo sapiens
234 TRIM73  
Two-hybrid Homo sapiens
235 HSPBP1 23640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
236 NF2 4771
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
237 KRT34  
Two-hybrid Homo sapiens
238 PTPRN2  
Proximity Label-MS Homo sapiens
239 TRIML2  
Two-hybrid Homo sapiens
240 PSMB4 5692
Two-hybrid Homo sapiens
241 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
242 PRPH 5630
Proximity Label-MS Homo sapiens
243 ZFYVE16  
Affinity Capture-Western Homo sapiens
244 PARK2  
Affinity Capture-MS Homo sapiens
245 CCDC33 80125
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
246 MEIS3  
Two-hybrid Homo sapiens
247 DNAJC5 80331
Proximity Label-MS Homo sapiens
248 POU6F2  
Two-hybrid Homo sapiens
249 MAPT  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
250 DIRAS3  
Proximity Label-MS Homo sapiens
251 STMN3  
Two-hybrid Homo sapiens
252 NTRK1 4914
Affinity Capture-MS Homo sapiens
253 SMAD2 4087
Affinity Capture-Western Homo sapiens
254 TGM1 7051
Affinity Capture-MS Homo sapiens
255 PELP1 27043
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
256 LINC00238  
Two-hybrid Homo sapiens
257 SF3A3 10946
Co-fractionation Homo sapiens
258 HNRNPDL 9987
Reconstituted Complex Homo sapiens
259 SCRN1 9805
Reconstituted Complex Homo sapiens
260 CSTF2T  
Two-hybrid Homo sapiens
261 BAG2 9532
Affinity Capture-MS Homo sapiens
262 TRIM10  
Two-hybrid Homo sapiens
263 FOXI1  
Two-hybrid Homo sapiens
264 VEGFA 7422
Affinity Capture-Western Homo sapiens
265 PEF1 553115
Two-hybrid Homo sapiens
266 MYO5A 4644
Affinity Capture-Western Homo sapiens
267 NUP54 53371
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
268 ODAM  
Two-hybrid Homo sapiens
269 MAP3K7  
Reconstituted Complex Homo sapiens
270 IFNA16  
Two-hybrid Homo sapiens
271 GATC  
Two-hybrid Homo sapiens
272 KCNE3  
Affinity Capture-MS Homo sapiens
273 NADSYN1 55191
Two-hybrid Homo sapiens
274 CELA2A  
Affinity Capture-MS Homo sapiens
275 RELT  
Affinity Capture-MS Homo sapiens
276 RAB9A 9367
Proximity Label-MS Homo sapiens
277 DGCR6  
Two-hybrid Homo sapiens
278 BICD1 636
Proximity Label-MS Homo sapiens
279 PTPN23 25930
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
280 TLR4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
281 ROCK1 6093
Co-localization Homo sapiens
282 NEDD4L 23327
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
283 SUMO1 7341
Two-hybrid Homo sapiens
284 AQP9  
Affinity Capture-MS Homo sapiens
285 PAK1 5058
Reconstituted Complex Homo sapiens
286 FAM166A  
Two-hybrid Homo sapiens
287 TUBB2A 7280
Reconstituted Complex Homo sapiens
288 KRT36 8689
Two-hybrid Homo sapiens
289 EXOC8 149371
Two-hybrid Homo sapiens
290 POU2AF1  
Two-hybrid Homo sapiens
291 BST2 684
Affinity Capture-Western Homo sapiens
292 LITAF 9516
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
293 MKRN3  
Two-hybrid Homo sapiens
294 INSR 3643
Affinity Capture-Western Homo sapiens
295 UBXN11  
Two-hybrid Homo sapiens
296 PSMB11 122706
Two-hybrid Homo sapiens
297 C11orf52 91894
Proximity Label-MS Homo sapiens
298 MTSS1 9788
Affinity Capture-Western Homo sapiens
299 TRAP1 10131
Reconstituted Complex Homo sapiens
300 SPC25 57405
Two-hybrid Homo sapiens
301 ATXN1L  
Two-hybrid Homo sapiens
302 C20orf195  
Two-hybrid Homo sapiens
303 TRIM23 373
Two-hybrid Homo sapiens
304 GUCA1C  
Two-hybrid Homo sapiens
305 CIT 11113
Affinity Capture-Western Homo sapiens
306 OSBPL5 114879
Reconstituted Complex Homo sapiens
307 RANGRF  
Co-fractionation Homo sapiens
308 SERBP1 26135
Affinity Capture-MS Homo sapiens
309 ICA1L  
Two-hybrid Homo sapiens
310 SNX2 6643
Co-fractionation Homo sapiens
311 TMCC2  
Reconstituted Complex Homo sapiens
312 STK32C  
Two-hybrid Homo sapiens
313 CAV1 857
Proximity Label-MS Homo sapiens
314 NOTCH1 4851
Affinity Capture-Western Homo sapiens
315 CTLA4  
Affinity Capture-MS Homo sapiens
316 INTS4  
Two-hybrid Homo sapiens
317 RIT1 6016
Negative Genetic Homo sapiens
318 VPS35 55737
Affinity Capture-Western Homo sapiens
319 SNX3 8724
Co-fractionation Homo sapiens
320 SCNN1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
321 ZNF34  
Two-hybrid Homo sapiens
322 TSG101 7251
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
323 BFSP2  
Two-hybrid Homo sapiens
324 HGH1 51236
Co-fractionation Homo sapiens
325 PRR5  
Two-hybrid Homo sapiens
326 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
327 TOMM22 56993
Co-fractionation Homo sapiens
328 B3GAT1  
Proximity Label-MS Homo sapiens
329 UBB  
Co-crystal Structure Bos taurus
Reconstituted Complex Bos taurus
330 HRASLS  
Two-hybrid Homo sapiens
331 JAG1 182
Affinity Capture-Western Homo sapiens
332 SCNN1B 6338
Affinity Capture-Western Homo sapiens
333 UBQLN1 29979
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
334 TOM1L1 10040
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
335 MED22  
Two-hybrid Homo sapiens
336 STX6 10228
Proximity Label-MS Homo sapiens
337 MARK4  
Reconstituted Complex Homo sapiens
338 SEC23A 10484
Co-fractionation Homo sapiens
339 CTTNBP2NL  
Two-hybrid Homo sapiens
340 NEDD4 4734
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
341 LMO4  
Two-hybrid Homo sapiens
342 LAMTOR1 55004
Proximity Label-MS Homo sapiens
343 CCDC114  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
344 CASK 8573
Reconstituted Complex Homo sapiens
345 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
346 ARMC8 25852
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
347 APLP2 334
Reconstituted Complex Homo sapiens
348 PTCD3 55037
Reconstituted Complex Homo sapiens
349 UBE2I 7329
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
350 IL2RA  
Affinity Capture-Western Homo sapiens
351 C11orf1  
Two-hybrid Homo sapiens
352 FAM185A  
Two-hybrid Homo sapiens
353 SMAD3 4088
Affinity Capture-Western Homo sapiens
354 KRT75 9119
Two-hybrid Homo sapiens
355 WBSCR27  
Two-hybrid Homo sapiens
356 ARRDC3 57561
Affinity Capture-Western Homo sapiens
357 UBC 444874
Reconstituted Complex Bos taurus
358 ITCH 83737
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
359 MAT2A 4144
Reconstituted Complex Homo sapiens
360 SNX1 6642
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
361 IPO4 79711
Two-hybrid Homo sapiens
362 C9orf24  
Two-hybrid Homo sapiens
363 KRT33B 3884
Two-hybrid Homo sapiens
364 NDUFB10 4716
Two-hybrid Homo sapiens
365 STXBP1 6812
Reconstituted Complex Homo sapiens
366 ATG12  
Proximity Label-MS Homo sapiens
367 C20orf173  
Two-hybrid Homo sapiens
368 RPRD1A  
Two-hybrid Homo sapiens
369 MAP3K1 4214
Biochemical Activity Homo sapiens
370 NXF1 10482
Affinity Capture-RNA Homo sapiens
371 CRX  
Two-hybrid Homo sapiens
372 ASB12  
Two-hybrid Homo sapiens
373 PAX5  
Two-hybrid Homo sapiens
374 LINC00265  
Reconstituted Complex Homo sapiens
375 LRIG1 26018
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
376 ACTN3 89
Two-hybrid Homo sapiens
377 TRIM27  
Two-hybrid Homo sapiens
378 NFKB1 4790
Proximity Label-MS Homo sapiens
379 PPP1R32  
Two-hybrid Homo sapiens
380 USHBP1  
Two-hybrid Homo sapiens
381 CLTC 1213
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
382 KRT16 3868
Two-hybrid Homo sapiens
383 MTHFD1L 25902
Reconstituted Complex Homo sapiens
384 MAPK1IP1L 93487
Two-hybrid Homo sapiens
385 KRT3 3850
Two-hybrid Homo sapiens
386 MRPL42  
Affinity Capture-MS Homo sapiens
387 ARRB2 409
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
388 EFHC2  
Two-hybrid Homo sapiens
389 SPRY2 10253
Affinity Capture-Western Homo sapiens
390 TRAK1 22906
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
391 HAUS1  
Two-hybrid Homo sapiens
392 HSF4  
Two-hybrid Homo sapiens
393 VPS37C 55048
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
394 MED4 29079
Two-hybrid Homo sapiens
395 ADRA2C  
Two-hybrid Homo sapiens
396 HMGN5 79366
Affinity Capture-MS Homo sapiens
397 PITX1  
Two-hybrid Homo sapiens
398 ATP2A2 488
Reconstituted Complex Homo sapiens
399 MED30  
Two-hybrid Homo sapiens
400 EXOC3L1  
Two-hybrid Homo sapiens
401 RPS3A 6189
Reconstituted Complex Homo sapiens
402 MAGED1 9500
Two-hybrid Homo sapiens
403 MOV10 4343
Affinity Capture-RNA Homo sapiens
404 DAAM2 23500
Two-hybrid Homo sapiens
405 ARRB1 408
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
406 LRRC61 65999
Two-hybrid Homo sapiens
407 SPATA12  
Two-hybrid Homo sapiens
408 ACOT11 26027
Two-hybrid Homo sapiens
409 CDR2  
Two-hybrid Homo sapiens
410 CYB5R2 51700
Two-hybrid Homo sapiens
411 SERTAD3  
Two-hybrid Homo sapiens
412 NEDD8 4738
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which HGS is involved
PathwayEvidenceSource
Bacterial Infection Pathways TAS Reactome
Bacterial Infection Pathways IEA Reactome
Cargo recognition for clathrin-mediated endocytosis TAS Reactome
Clathrin-mediated endocytosis TAS Reactome
Deubiquitination TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
EGFR downregulation TAS Reactome
Endosomal Sorting Complex Required For Transport (ESCRT) TAS Reactome
Infection with Mycobacterium tuberculosis TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Inhibition of membrane repair TAS Reactome
InlB-mediated entry of Listeria monocytogenes into host cell IEA Reactome
Listeria monocytogenes entry into host cells IEA Reactome
Lysosome Vesicle Biogenesis TAS Reactome
Membrane Trafficking TAS Reactome
Metabolism of proteins TAS Reactome
Negative regulation of MET activity TAS Reactome
Post-translational protein modification TAS Reactome
Prevention of phagosomal-lysosomal fusion TAS Reactome
Response of Mtb to phagocytosis TAS Reactome
RHO GTPase cycle TAS Reactome
RHOBTB3 ATPase cycle TAS Reactome
RHOU GTPase cycle TAS Reactome
Signal Transduction TAS Reactome
Signaling by EGFR TAS Reactome
Signaling by MET TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Suppression of phagosomal maturation TAS Reactome
trans-Golgi Network Vesicle Budding TAS Reactome
Ub-specific processing proteases TAS Reactome
Vesicle-mediated transport TAS Reactome





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