Gene description for ROCK1
Gene name Rho-associated, coiled-coil containing protein kinase 1
Gene symbol ROCK1
Other names/aliases P160ROCK
ROCK-I
Species Homo sapiens
 Database cross references - ROCK1
ExoCarta ExoCarta_6093
Vesiclepedia VP_6093
Entrez Gene 6093
HGNC 10251
MIM 601702
UniProt Q13464  
 ROCK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for ROCK1
Molecular Function
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 NAS
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    small GTPase binding GO:0031267 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    tau protein binding GO:0048156 NAS
    tau-protein kinase activity GO:0050321 NAS
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IBA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IDA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    epithelial to mesenchymal transition GO:0001837 ISS
    aortic valve morphogenesis GO:0003180 ISS
    apical constriction GO:0003383 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    smooth muscle contraction GO:0006939 TAS
    leukocyte cell-cell adhesion GO:0007159 IDA
    signal transduction GO:0007165 TAS
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IGI
    positive regulation of autophagy GO:0010508 IGI
    positive regulation of cardiac muscle hypertrophy GO:0010613 IMP
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISS
    positive regulation of phosphatase activity GO:0010922 IMP
    negative regulation of angiogenesis GO:0016525 IMP
    peptidyl-serine phosphorylation GO:0018105 IDA
    membrane to membrane docking GO:0022614 IDA
    actin cytoskeleton organization GO:0030036 ISS
    regulation of cell adhesion GO:0030155 TAS
    regulation of cell migration GO:0030334 IDA
    cortical actin cytoskeleton organization GO:0030866 IBA
    actomyosin structure organization GO:0031032 IBA
    neuron projection development GO:0031175 IGI
    bleb assembly GO:0032060 IEA
    negative regulation of protein binding GO:0032091 IDA
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of actin cytoskeleton organization GO:0032956 IDA
    regulation of actin cytoskeleton organization GO:0032956 ISS
    regulation of actin cytoskeleton organization GO:0032956 TAS
    positive regulation of dephosphorylation GO:0035306 IMP
    positive regulation of MAPK cascade GO:0043410 IMP
    regulation of keratinocyte differentiation GO:0045616 IMP
    regulation of neuron differentiation GO:0045664 IGI
    embryonic morphogenesis GO:0048598 IBA
    leukocyte migration GO:0050900 IDA
    leukocyte tethering or rolling GO:0050901 IDA
    negative regulation of membrane protein ectodomain proteolysis GO:0051045 TAS
    myoblast migration GO:0051451 ISS
    regulation of stress fiber assembly GO:0051492 TAS
    regulation of focal adhesion assembly GO:0051893 TAS
    positive regulation of focal adhesion assembly GO:0051894 ISS
    mRNA destabilization GO:0061157 ISS
    negative regulation of biomineral tissue development GO:0070168 ISS
    regulation of microtubule cytoskeleton organization GO:0070507 IDA
    response to transforming growth factor beta GO:0071559 ISS
    protein localization to plasma membrane GO:0072659 IGI
    protein localization to plasma membrane GO:0072659 IMP
    regulation of synapse maturation GO:0090128 IEA
    podocyte cell migration GO:0090521 ISS
    motor neuron apoptotic process GO:0097049 IEA
    blood vessel diameter maintenance GO:0097746 ISS
    regulation of angiotensin-activated signaling pathway GO:0110061 IMP
    neuron projection arborization GO:0140058 IGI
    positive regulation of amyloid-beta clearance GO:1900223 IGI
    regulation of synaptic vesicle endocytosis GO:1900242 IGI
    regulation of cell junction assembly GO:1901888 IBA
    negative regulation of amyloid-beta formation GO:1902430 IMP
    negative regulation of amyloid precursor protein catabolic process GO:1902992 IMP
    regulation of establishment of endothelial barrier GO:1903140 IGI
    regulation of establishment of endothelial barrier GO:1903140 IMP
    negative regulation of bicellular tight junction assembly GO:1903347 IGI
    positive regulation of connective tissue replacement GO:1905205 IMP
    response to angiotensin GO:1990776 ISS
    regulation of establishment of cell polarity GO:2000114 TAS
    regulation of cell motility GO:2000145 TAS
    negative regulation of motor neuron apoptotic process GO:2000672 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    ruffle GO:0001726 ISS
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IBA
    centriole GO:0005814 IEA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    cytoskeleton GO:0005856 ISS
    plasma membrane GO:0005886 ISS
    cytoplasmic stress granule GO:0010494 IBA
    cytoplasmic stress granule GO:0010494 ISS
    lamellipodium GO:0030027 ISS
    bleb GO:0032059 IEA
    secretory granule lumen GO:0034774 TAS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
 Experiment description of studies that identified ROCK1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ROCK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SPRTN  
Affinity Capture-MS Homo sapiens
3 HDAC2 3066
Affinity Capture-MS Homo sapiens
4 MED17  
Affinity Capture-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 SYCE1  
Affinity Capture-MS Homo sapiens
7 YWHAE 7531
Affinity Capture-MS Homo sapiens
8 TSG101 7251
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 FAM21A 387680
Co-fractionation Homo sapiens
10 LAMP2 3920
Proximity Label-MS Homo sapiens
11 CFL1 1072
Affinity Capture-Western Homo sapiens
12 LMO2  
Two-hybrid Homo sapiens
13 MLLT4 4301
Proximity Label-MS Homo sapiens
14 MON2 23041
Co-fractionation Homo sapiens
15 CASP3 836
Biochemical Activity Homo sapiens
16 ANKRD49  
Affinity Capture-MS Homo sapiens
17 IMPDH2 3615
Proximity Label-MS Homo sapiens
18 HGS 9146
Co-localization Homo sapiens
19 LINC00452  
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
20 ADD1 118
Co-fractionation Homo sapiens
21 RHOA 387
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
22 PFN2 5217
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 SMAD5 4090
Biochemical Activity Homo sapiens
24 RAB35 11021
Proximity Label-MS Homo sapiens
25 ANAPC16  
Affinity Capture-MS Homo sapiens
26 TRIM15  
PCA Homo sapiens
27 NEIL3  
Affinity Capture-MS Homo sapiens
28 LAMTOR1 55004
Proximity Label-MS Homo sapiens
29 Snap29  
Affinity Capture-MS Mus musculus
30 UBE2T  
Biochemical Activity Homo sapiens
31 TPPP 11076
Biochemical Activity Homo sapiens
32 LYN 4067
Proximity Label-MS Homo sapiens
33 MCAM 4162
Proximity Label-MS Homo sapiens
34 RAB4A 5867
Proximity Label-MS Homo sapiens
35 CERS2 29956
Affinity Capture-MS Homo sapiens
36 ADD2 119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CDH1 999
Co-fractionation Homo sapiens
38 FAM124A  
Two-hybrid Homo sapiens
39 BBS1 582
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 DCAF7 10238
Affinity Capture-MS Homo sapiens
42 SLC9A1 6548
Affinity Capture-Western Homo sapiens
43 CDKN3 1033
Affinity Capture-Western Homo sapiens
44 IL20RA  
Affinity Capture-MS Homo sapiens
45 SMAD2 4087
Two-hybrid Homo sapiens
46 KIAA1033 23325
Co-fractionation Homo sapiens
47 CYLD  
Affinity Capture-MS Homo sapiens
48 C6orf165  
Two-hybrid Homo sapiens
49 HMGB2 3148
Two-hybrid Homo sapiens
50 ANLN 54443
Affinity Capture-MS Homo sapiens
51 RNF40 9810
Co-fractionation Homo sapiens
52 ZBTB2 57621
Affinity Capture-MS Homo sapiens
53 KDM4D  
Affinity Capture-MS Homo sapiens
54 CHERP 10523
Affinity Capture-MS Homo sapiens
55 LAMP1 3916
Proximity Label-MS Homo sapiens
56 MAGEB4  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 Smad3  
Affinity Capture-MS Mus musculus
58 CUL3 8452
Affinity Capture-MS Homo sapiens
59 ROCK2 9475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
60 ELAC2 60528
Cross-Linking-MS (XL-MS) Homo sapiens
61 ARF6 382
Proximity Label-MS Homo sapiens
62 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
63 SNX27 81609
Affinity Capture-MS Homo sapiens
64 HTRA3 94031
Affinity Capture-MS Homo sapiens
65 SEC24A 10802
Co-fractionation Homo sapiens
66 NXF1 10482
Affinity Capture-RNA Homo sapiens
67 RND3 390
Reconstituted Complex Homo sapiens
68 ANKFY1 51479
Co-fractionation Homo sapiens
69 AHR 196
Affinity Capture-MS Homo sapiens
70 RHOBTB1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
71 TNS2 23371
Affinity Capture-MS Homo sapiens
72 NDC80 10403
Co-fractionation Homo sapiens
73 RHOB 388
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
74 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
75 LMNA 4000
Co-fractionation Homo sapiens
76 TAF4 6874
Affinity Capture-MS Homo sapiens
77 FLOT1 10211
Proximity Label-MS Homo sapiens
78 XRCC5 7520
Affinity Capture-MS Homo sapiens
79 CDC25A  
Biochemical Activity Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 GAB1  
Co-crystal Structure Homo sapiens
82 PARP1 142
Proximity Label-MS Homo sapiens
83 HEXA 3073
Co-fractionation Homo sapiens
84 CASP7  
Biochemical Activity Homo sapiens
85 TP53 7157
Affinity Capture-MS Homo sapiens
86 RHOC 389
Affinity Capture-Western Homo sapiens
87 Dync1li1 235661
Affinity Capture-MS Mus musculus
88 LONP1 9361
Co-fractionation Homo sapiens
89 DFFA 1676
Co-fractionation Homo sapiens
90 EDEM1  
Affinity Capture-MS Homo sapiens
91 SMURF1 57154
Affinity Capture-MS Homo sapiens
92 CDK15  
Affinity Capture-MS Homo sapiens
93 SMAD4  
Affinity Capture-MS Homo sapiens
94 LIMK2 3985
Biochemical Activity Homo sapiens
95 UFL1 23376
Affinity Capture-MS Homo sapiens
96 PHKA2 5256
Affinity Capture-MS Homo sapiens
97 ASMTL 8623
Co-fractionation Homo sapiens
98 ITSN2 50618
Two-hybrid Homo sapiens
99 POLR3C 10623
Two-hybrid Homo sapiens
100 RAB2A 5862
Proximity Label-MS Homo sapiens
101 ARRB1 408
Affinity Capture-MS Homo sapiens
102 FECH 2235
Affinity Capture-MS Homo sapiens
103 EPHA2 1969
Proximity Label-MS Homo sapiens
104 KRAS 3845
Proximity Label-MS Homo sapiens
105 NFIC 4782
Co-fractionation Homo sapiens
106 DIAPH1 1729
Co-fractionation Homo sapiens
107 IKBKB 3551
Biochemical Activity Homo sapiens
108 PRMT1 3276
Affinity Capture-MS Homo sapiens
109 NOTCH1 4851
Affinity Capture-MS Homo sapiens
110 CUL4A 8451
Affinity Capture-MS Homo sapiens
111 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ROCK1 is involved
PathwayEvidenceSource
Apoptosis TAS Reactome
Apoptotic cleavage of cellular proteins TAS Reactome
Apoptotic execution phase TAS Reactome
Axon guidance IEA Reactome
Axon guidance TAS Reactome
Developmental Biology IEA Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
EPH-Ephrin signaling IEA Reactome
EPH-Ephrin signaling TAS Reactome
EPHA-mediated growth cone collapse IEA Reactome
EPHB-mediated forward signaling IEA Reactome
EPHB-mediated forward signaling TAS Reactome
G alpha (12/13) signalling events TAS Reactome
GPCR downstream signalling TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Nervous system development IEA Reactome
Nervous system development TAS Reactome
Neutrophil degranulation TAS Reactome
Potential therapeutics for SARS TAS Reactome
Programmed Cell Death TAS Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate ROCKs IEA Reactome
RHO GTPases Activate ROCKs TAS Reactome
RHOA GTPase cycle TAS Reactome
RHOB GTPase cycle TAS Reactome
RHOBTB GTPase Cycle TAS Reactome
RHOBTB1 GTPase cycle TAS Reactome
RHOC GTPase cycle TAS Reactome
RHOH GTPase cycle TAS Reactome
RND3 GTPase cycle TAS Reactome
SARS-CoV Infections TAS Reactome
Sema4D in semaphorin signaling IEA Reactome
Sema4D in semaphorin signaling TAS Reactome
Sema4D induced cell migration and growth-cone collapse IEA Reactome
Sema4D induced cell migration and growth-cone collapse TAS Reactome
Semaphorin interactions IEA Reactome
Semaphorin interactions TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by GPCR TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by VEGF TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
Viral Infection Pathways TAS Reactome





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