Gene ontology annotations for ADD1
Experiment description of studies that identified ADD1 in sEVs
1
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
9
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
10
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
11
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
15
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
16
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
17
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for ADD1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
OCLN
100506658
Proximity Label-MS
Homo sapiens
2
TK1
7083
Two-hybrid
Homo sapiens
3
MARCKS
4082
Proximity Label-MS
Homo sapiens
4
DYNC1I2
1781
Co-fractionation
Homo sapiens
5
TPM2
7169
Affinity Capture-MS
Homo sapiens
6
RPA2
6118
Affinity Capture-MS
Homo sapiens
7
ACTC1
70
Affinity Capture-MS
Homo sapiens
Proximity Label-MS
Homo sapiens
8
C9orf78
51759
Affinity Capture-MS
Homo sapiens
9
ADD3
120
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
10
ROCK2
9475
Co-fractionation
Homo sapiens
11
CALM1
801
Phenotypic Suppression
Homo sapiens
12
GBP2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
13
PNKD
Affinity Capture-MS
Homo sapiens
14
MAP1LC3B
81631
Two-hybrid
Homo sapiens
15
ANKRD29
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
16
TLN1
7094
Co-fractionation
Homo sapiens
17
CADPS
Affinity Capture-MS
Homo sapiens
18
IQGAP1
8826
Affinity Capture-MS
Homo sapiens
19
STX7
8417
Proximity Label-MS
Homo sapiens
20
HSPH1
10808
Affinity Capture-Western
Homo sapiens
21
FAM78A
Affinity Capture-MS
Homo sapiens
22
C11orf52
91894
Proximity Label-MS
Homo sapiens
23
PLCG1
5335
Co-fractionation
Homo sapiens
24
HMG20A
Two-hybrid
Homo sapiens
25
RAB35
11021
Proximity Label-MS
Homo sapiens
26
PLCB3
5331
Co-fractionation
Homo sapiens
27
CSNK2B
1460
Two-hybrid
Homo sapiens
28
EBAG9
9166
Proximity Label-MS
Homo sapiens
29
Lima1
Affinity Capture-MS
Mus musculus
30
LAMTOR1
55004
Proximity Label-MS
Homo sapiens
31
FAS
355
Proximity Label-MS
Homo sapiens
32
ZDHHC17
23390
Two-hybrid
Homo sapiens
33
NSUN2
54888
Co-fractionation
Homo sapiens
34
CDKN2AIP
Affinity Capture-MS
Homo sapiens
35
SPTB
6710
Reconstituted Complex
Homo sapiens
Co-localization
Homo sapiens
36
CXADR
1525
Proximity Label-MS
Homo sapiens
37
TMOD1
Affinity Capture-MS
Homo sapiens
38
LYN
4067
Proximity Label-MS
Homo sapiens
39
MAPRE1
22919
Affinity Capture-MS
Homo sapiens
40
MCAM
4162
Proximity Label-MS
Homo sapiens
41
DNAJC5
80331
Proximity Label-MS
Homo sapiens
42
ADD2
119
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
43
LRWD1
Affinity Capture-MS
Homo sapiens
44
CDH1
999
Proximity Label-MS
Homo sapiens
45
VCP
7415
Affinity Capture-MS
Homo sapiens
46
NTRK1
4914
Affinity Capture-MS
Homo sapiens
47
ACTB
60
Proximity Label-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
48
Coro1c
23790
Affinity Capture-MS
Mus musculus
49
GEMIN7
Two-hybrid
Homo sapiens
50
ACTG1
71
Affinity Capture-MS
Homo sapiens
51
DST
667
Affinity Capture-MS
Homo sapiens
52
Myh9
17886
Affinity Capture-MS
Mus musculus
53
XPO1
7514
Affinity Capture-MS
Homo sapiens
54
EPB41L4A
64097
Proximity Label-MS
Homo sapiens
55
LAMP1
3916
Proximity Label-MS
Homo sapiens
56
ATP6V1D
51382
Affinity Capture-MS
Homo sapiens
57
RNF138
Affinity Capture-MS
Homo sapiens
58
ARF6
382
Proximity Label-MS
Homo sapiens
59
SNX27
81609
Affinity Capture-MS
Homo sapiens
60
MYO1C
4641
Affinity Capture-MS
Homo sapiens
61
HEXA
3073
Co-fractionation
Homo sapiens
62
RPA3
6119
Affinity Capture-MS
Homo sapiens
Proximity Label-MS
Homo sapiens
63
NDC80
10403
Co-fractionation
Homo sapiens
64
NUBP1
4682
Co-fractionation
Homo sapiens
65
LMNA
4000
Co-fractionation
Homo sapiens
66
TPM4
7171
Affinity Capture-MS
Homo sapiens
67
FLOT1
10211
Proximity Label-MS
Homo sapiens
68
TPM3
7170
Affinity Capture-MS
Homo sapiens
69
NES
10763
Affinity Capture-MS
Homo sapiens
70
SPTBN1
6711
Affinity Capture-MS
Homo sapiens
71
NUP54
53371
Affinity Capture-MS
Homo sapiens
72
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
73
SPTAN1
6709
Affinity Capture-MS
Homo sapiens
74
Tmod3
50875
Affinity Capture-MS
Mus musculus
75
BKRF1
Affinity Capture-MS
76
ROCK1
6093
Co-fractionation
Homo sapiens
77
SVIL
6840
Affinity Capture-MS
Homo sapiens
78
TP53
7157
Affinity Capture-MS
Homo sapiens
79
ATL2
64225
Affinity Capture-MS
Homo sapiens
80
RPA1
6117
Affinity Capture-MS
Homo sapiens
81
Myo1c
17913
Affinity Capture-MS
Mus musculus
82
PDHA1
5160
Affinity Capture-MS
Homo sapiens
83
CAPZA2
830
Affinity Capture-MS
Homo sapiens
84
DPPA4
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
85
DDX58
23586
Affinity Capture-RNA
Homo sapiens
86
UBIAD1
29914
Affinity Capture-MS
Homo sapiens
87
ASMTL
8623
Co-fractionation
Homo sapiens
88
XPO5
57510
Co-fractionation
Homo sapiens
89
RAB2A
5862
Proximity Label-MS
Homo sapiens
90
INF2
64423
Affinity Capture-MS
Homo sapiens
91
PRKCA
5578
Biochemical Activity
Homo sapiens
92
EPHA2
1969
Proximity Label-MS
Homo sapiens
93
KRAS
3845
Proximity Label-MS
Homo sapiens
94
HSPG2
3339
Affinity Capture-MS
Homo sapiens
95
CAV1
857
Proximity Label-MS
Homo sapiens
96
Tpm4
Affinity Capture-MS
Mus musculus
97
CUL4A
8451
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which ADD1 is involved