Gene description for INSR
Gene name insulin receptor
Gene symbol INSR
Other names/aliases CD220
HHF5
Species Homo sapiens
 Database cross references - INSR
ExoCarta ExoCarta_3643
Vesiclepedia VP_3643
Entrez Gene 3643
HGNC 6091
MIM 147670
UniProt P06213  
 INSR identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for INSR
Molecular Function
    amyloid-beta binding GO:0001540 IPI
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 IMP
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IBA
    insulin receptor activity GO:0005009 IDA
    insulin receptor activity GO:0005009 TAS
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    insulin-like growth factor receptor binding GO:0005159 IDA
    structural molecule activity GO:0005198 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    GTP binding GO:0005525 IDA
    boss receptor activity GO:0008288 IEA
    protein domain specific binding GO:0019904 IPI
    protein kinase activator activity GO:0030295 IMP
    insulin-like growth factor I binding GO:0031994 IPI
    insulin-like growth factor II binding GO:0031995 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    cargo receptor activity GO:0038024 ISS
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    identical protein binding GO:0042802 IPI
    phosphatidylinositol 3-kinase binding GO:0043548 IPI
    insulin binding GO:0043559 IDA
    insulin binding GO:0043559 IPI
    insulin receptor substrate binding GO:0043560 IBA
    insulin receptor substrate binding GO:0043560 IPI
    protein-containing complex binding GO:0044877 IPI
    PTB domain binding GO:0051425 IPI
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 IDA
    positive regulation of receptor internalization GO:0002092 IDA
    heart morphogenesis GO:0003007 IMP
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated transcription GO:0006355 IMP
    protein phosphorylation GO:0006468 TAS
    receptor-mediated endocytosis GO:0006898 ISS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    multicellular organism development GO:0007275 IBA
    learning GO:0007612 TAS
    memory GO:0007613 TAS
    positive regulation of cell population proliferation GO:0008284 IC
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IMP
    insulin receptor signaling pathway GO:0008286 IDA
    insulin receptor signaling pathway GO:0008286 ISS
    insulin receptor signaling pathway GO:0008286 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    epidermis development GO:0008544 IEA
    male gonad development GO:0008584 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    male sex determination GO:0030238 IEA
    adrenal gland development GO:0030325 IEA
    positive regulation of cell migration GO:0030335 IMP
    exocrine pancreas development GO:0031017 IEA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    receptor internalization GO:0031623 IEA
    activation of protein kinase B activity GO:0032148 IDA
    cellular response to insulin stimulus GO:0032869 IDA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    glucose homeostasis GO:0042593 IBA
    glucose homeostasis GO:0042593 IMP
    positive regulation of protein-containing complex disassembly GO:0043243 ISS
    positive regulation of MAP kinase activity GO:0043406 IMP
    positive regulation of MAPK cascade GO:0043410 IBA
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of MAPK cascade GO:0043410 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    positive regulation of glycogen biosynthetic process GO:0045725 IDA
    positive regulation of glycolytic process GO:0045821 IMP
    positive regulation of mitotic nuclear division GO:0045840 IMP
    positive regulation of DNA-templated transcription GO:0045893 IEA
    regulation of embryonic development GO:0045995 IMP
    positive regulation of D-glucose import GO:0046326 IDA
    positive regulation of D-glucose import GO:0046326 NAS
    symbiont entry into host cell GO:0046718 IMP
    protein autophosphorylation GO:0046777 IDA
    protein autophosphorylation GO:0046777 IMP
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    positive regulation of developmental growth GO:0048639 IMP
    positive regulation of meiotic cell cycle GO:0051446 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IBA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    positive regulation of respiratory burst GO:0060267 IDA
    dendritic spine maintenance GO:0097062 ISS
    amyloid-beta clearance GO:0097242 ISS
    transport across blood-brain barrier GO:0150104 NAS
    neuron projection maintenance GO:1990535 ISS
    regulation of female gonad development GO:2000194 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    lysosome GO:0005764 IEA
    late endosome GO:0005770 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IC
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    insulin receptor complex GO:0005899 IBA
    insulin receptor complex GO:0005899 IDA
    insulin receptor complex GO:0005899 IMP
    caveola GO:0005901 IDA
    external side of plasma membrane GO:0009897 ISS
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 IDA
    axon GO:0030424 IBA
    dendrite GO:0030425 ISS
    nuclear lumen GO:0031981 IDA
    dendrite membrane GO:0032590 ISS
    neuronal cell body membrane GO:0032809 ISS
    neuronal cell body GO:0043025 ISS
    receptor complex GO:0043235 IDA
    cell body GO:0044297 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified INSR in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
13
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
14
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for INSR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HACD3 51495
Affinity Capture-Western Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 Irs1 25467
Biochemical Activity Rattus norvegicus
4 Grb14  
Affinity Capture-Western Rattus norvegicus
5 CLEC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SHC1 6464
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
7 STAT5B 6777
Affinity Capture-Western Homo sapiens
8 PRKCD 5580
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CSK 1445
Two-hybrid Homo sapiens
10 LY6H  
Affinity Capture-MS Homo sapiens
11 STAT5A 6776
Affinity Capture-Western Homo sapiens
12 GJA1 2697
Proximity Label-MS Homo sapiens
13 HRAS 3265
Biochemical Activity Homo sapiens
14 LGALS1 3956
Affinity Capture-MS Homo sapiens
15 SOCS1  
Affinity Capture-Western Homo sapiens
16 GRB10 2887
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 PTPN1 5770
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
18 SLURP1  
Affinity Capture-MS Homo sapiens
19 SNX6 58533
Affinity Capture-Western Homo sapiens
20 MOK  
Affinity Capture-MS Homo sapiens
21 GRB7 2886
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
22 PLA2G5  
Affinity Capture-MS Homo sapiens
23 INSRR 3645
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 CARKD 55739
Affinity Capture-MS Homo sapiens
25 AHSG 197
Reconstituted Complex Homo sapiens
26 HGS 9146
Affinity Capture-Western Homo sapiens
27 KHDRBS1 10657
Affinity Capture-Western Homo sapiens
28 PLCG1 5335
Protein-peptide Homo sapiens
29 KRT31 3881
Two-hybrid Homo sapiens
30 TEAD1  
Two-hybrid Homo sapiens
31 IGF2 3481
Affinity Capture-MS Homo sapiens
32 OCLN 100506658
Proximity Label-MS Homo sapiens
33 ENPP1 5167
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 IGFL3  
Affinity Capture-MS Homo sapiens
35 HSD17B2 3294
Affinity Capture-MS Homo sapiens
36 LGALS9 3965
Affinity Capture-MS Homo sapiens
37 SOCS3 9021
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 BTNL2  
Affinity Capture-MS Homo sapiens
39 TMEM106A  
Affinity Capture-MS Homo sapiens
40 ERBB2 2064
PCA Homo sapiens
41 MPZL2 10205
Affinity Capture-Western Homo sapiens
42 MAD2L1 4085
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
43 SNX4 8723
Affinity Capture-Western Homo sapiens
44 Socs1  
Affinity Capture-Western Rattus norvegicus
45 LAMTOR1 55004
Proximity Label-MS Homo sapiens
46 SORBS1 10580
Affinity Capture-Western Homo sapiens
47 DUSP18  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
48 MAPK1 5594
Affinity Capture-Western Homo sapiens
49 MARCH1  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 FAM19A4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 VAV1 7409
Reconstituted Complex Homo sapiens
52 MAPK3 5595
Affinity Capture-Western Homo sapiens
53 IGF1R 3480
Affinity Capture-MS Homo sapiens
54 IRF7  
Two-hybrid Homo sapiens
55 SOCS6  
Affinity Capture-Western Homo sapiens
56 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SH2B2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
58 KIF5A 3798
Affinity Capture-Western Homo sapiens
59 IDS  
Affinity Capture-MS Homo sapiens
60 SDF2L1 23753
Affinity Capture-MS Homo sapiens
61 NTRK1 4914
Affinity Capture-MS Homo sapiens
62 PPM1A 5494
Affinity Capture-Western Homo sapiens
63 SYNCRIP 10492
Biochemical Activity Homo sapiens
64 SMAD2 4087
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
65 DHFRL1  
Affinity Capture-MS Homo sapiens
66 PATE1  
Affinity Capture-MS Homo sapiens
67 FAM19A2 338811
Affinity Capture-MS Homo sapiens
68 NMS  
Affinity Capture-MS Homo sapiens
69 LY86  
Affinity Capture-MS Homo sapiens
70 SNX1 6642
Affinity Capture-Western Homo sapiens
71 DNAJC25-GNG10  
Affinity Capture-MS Homo sapiens
72 GPIHBP1  
Affinity Capture-MS Homo sapiens
73 PTPN11 5781
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
74 DUSP19  
Two-hybrid Homo sapiens
75 ADAMTS4  
Affinity Capture-MS Homo sapiens
76 Hgf  
Affinity Capture-Western Rattus norvegicus
77 FRS2 10818
Biochemical Activity Homo sapiens
78 USP4 7375
Affinity Capture-Western Homo sapiens
79 ECEL1  
Affinity Capture-MS Homo sapiens
80 HSD11B1 3290
Affinity Capture-MS Homo sapiens
81 PTPN6 5777
Affinity Capture-Western Homo sapiens
82 C1QA 712
Affinity Capture-MS Homo sapiens
83 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PRKAA2 5563
Affinity Capture-Western Homo sapiens
85 CUL3 8452
Affinity Capture-MS Homo sapiens
86 RLN1  
Affinity Capture-MS Homo sapiens
87 LYZL1  
Affinity Capture-MS Homo sapiens
88 SNX27 81609
Affinity Capture-Western Homo sapiens
89 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 DNASE1L2  
Affinity Capture-MS Homo sapiens
91 Stat5b  
Biochemical Activity Mus musculus
92 SNX17 9784
Affinity Capture-Western Homo sapiens
93 FBXO2 26232
Affinity Capture-MS Homo sapiens
94 ZDHHC18 84243
Affinity Capture-MS Homo sapiens
95 SH2B1 25970
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
96 FCGR2B  
Affinity Capture-MS Homo sapiens
97 HYOU1 10525
Affinity Capture-MS Homo sapiens
98 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
99 RHOB 388
Proximity Label-MS Homo sapiens
100 STYX  
Two-hybrid Homo sapiens
101 Mad2l1 56150
Affinity Capture-MS Mus musculus
102 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
103 ATIC 471
Affinity Capture-Western Homo sapiens
104 PPM1F 9647
Affinity Capture-Western Homo sapiens
105 STUB1 10273
Affinity Capture-Western Homo sapiens
106 RASA1 5921
Affinity Capture-Western Homo sapiens
107 PRKAA1 5562
Affinity Capture-Western Homo sapiens
108 ARRB2 409
Affinity Capture-Western Homo sapiens
109 GRB2 2885
Affinity Capture-Western Homo sapiens
110 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 SSH1  
Affinity Capture-Western Homo sapiens
112 INPPL1 3636
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
113 IRS2 8660
Two-hybrid Homo sapiens
114 GRB14 2888
Affinity Capture-Western Homo sapiens
115 Grb2  
Affinity Capture-Western Rattus norvegicus
116 SQSTM1 8878
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
117 INSR 3643
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
118 ZDHHC23  
Affinity Capture-MS Homo sapiens
119 C11orf52 91894
Proximity Label-MS Homo sapiens
120 DOK1 1796
Biochemical Activity Homo sapiens
121 C1QB 713
Affinity Capture-MS Homo sapiens
122 WNT3A 89780
Affinity Capture-MS Homo sapiens
123 DEFB135  
Affinity Capture-MS Homo sapiens
124 Socs6  
Affinity Capture-Western Rattus norvegicus
125 EPHA2 1969
Proximity Label-MS Homo sapiens
126 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 SNX2 6643
Affinity Capture-Western Homo sapiens
128 KRAS 3845
Proximity Label-MS Homo sapiens
129 UNC5CL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 PTPRR  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
131 VAV3 10451
Affinity Capture-Western Homo sapiens
132 JAK2 3717
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
133 IRS1 3667
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
134 CAV1 857
Affinity Capture-Western Homo sapiens
135 JAK1 3716
Affinity Capture-Western Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here