Gene description for SNX4
Gene name sorting nexin 4
Gene symbol SNX4
Other names/aliases ATG24B
Species Homo sapiens
 Database cross references - SNX4
ExoCarta ExoCarta_8723
Vesiclepedia VP_8723
Entrez Gene 8723
HGNC 11175
MIM 605931
UniProt O95219  
 SNX4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SNX4
Molecular Function
    epidermal growth factor receptor binding GO:0005154 IDA
    insulin receptor binding GO:0005158 IDA
    protein binding GO:0005515 IPI
    phosphatidylinositol-3-phosphate binding GO:0032266 IBA
    phosphatidylinositol-3-phosphate binding GO:0032266 IMP
    phosphatidylinositol binding GO:0035091 IMP
    transferrin receptor binding GO:1990459 IDA
    leptin receptor binding GO:1990460 IDA
Biological Process
    protein transport GO:0015031 IBA
    protein transport GO:0015031 IMP
    endocytic recycling GO:0032456 IMP
    positive regulation of histamine secretion by mast cell GO:1903595 IMP
    positive regulation of autophagosome assembly GO:2000786 IBA
    positive regulation of autophagosome assembly GO:2000786 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 IDA
    cytoplasmic dynein complex GO:0005868 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IDA
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 IDA
    early endosome membrane GO:0031901 IBA
    early endosome membrane GO:0031901 IDA
    early endosome membrane GO:0031901 IDA
    protein-containing complex GO:0032991 IDA
    presynaptic endosome GO:0098830 IEA
 Experiment description of studies that identified SNX4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SNX4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MECR 51102
Affinity Capture-MS Homo sapiens
2 SNX1 6642
Co-fractionation Homo sapiens
3 DYNC1H1 1778
Co-fractionation Homo sapiens
4 SNX7 51375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 DYNLT1 6993
Co-fractionation Homo sapiens
6 NPAS1  
Affinity Capture-MS Homo sapiens
7 SNX30 401548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 DYNLL1 8655
Co-fractionation Homo sapiens
10 TPR 7175
Co-fractionation Homo sapiens
11 PIP5K1C  
Affinity Capture-MS Homo sapiens
12 PDGFRA 5156
Affinity Capture-Western Homo sapiens
13 NUDCD3 23386
Co-fractionation Homo sapiens
14 INSR 3643
Affinity Capture-Western Homo sapiens
15 NPEPPS 9520
Affinity Capture-MS Homo sapiens
16 INTS2  
Co-fractionation Homo sapiens
17 EGFR 1956
Affinity Capture-Western Homo sapiens
18 PDGFRB 5159
Affinity Capture-Western Homo sapiens
19 METTL21B  
Affinity Capture-MS Homo sapiens
20 SUPT5H 6829
Two-hybrid Homo sapiens
21 HIVEP1 3096
Two-hybrid Homo sapiens
22 SNX6 58533
Affinity Capture-Western Homo sapiens
23 DNAJB6 10049
Affinity Capture-MS Homo sapiens
24 LEPR 3953
Affinity Capture-Western Homo sapiens
25 PTPN5  
Two-hybrid Homo sapiens
26 XPO5 57510
Co-fractionation Homo sapiens
27 C10orf88  
Affinity Capture-MS Homo sapiens
28 ANKMY2 57037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RPA2 6118
Proximity Label-MS Homo sapiens
30 DYNC1LI1 51143
Co-fractionation Homo sapiens
31 OGT 8473
Reconstituted Complex Homo sapiens
32 RAB7A 7879
Proximity Label-MS Homo sapiens
33 COPS6 10980
Affinity Capture-MS Homo sapiens
34 ARL6IP1 23204
Two-hybrid Homo sapiens
35 BICD2 23299
Proximity Label-MS Homo sapiens
36 BICD1 636
Proximity Label-MS Homo sapiens
37 SNX2 6643
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
38 MAGEA6  
Two-hybrid Homo sapiens
39 ABCB10 23456
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ATP6V0A1 535
Two-hybrid Homo sapiens
41 DUSP18  
Proximity Label-MS Homo sapiens
42 DGAT2L6  
Two-hybrid Homo sapiens
43 CCT8L2  
Affinity Capture-MS Homo sapiens
44 RPA3 6119
Proximity Label-MS Homo sapiens
45 DYNLT3 6990
Co-fractionation Homo sapiens
46 BIN1 274
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
47 DRD2  
Affinity Capture-MS Homo sapiens
48 CACYBP 27101
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SNX4 is involved
No pathways found





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