Gene description for ARL6IP1
Gene name ADP-ribosylation factor-like 6 interacting protein 1
Gene symbol ARL6IP1
Other names/aliases AIP1
ARL6IP
ARMER
SPG61
Species Homo sapiens
 Database cross references - ARL6IP1
ExoCarta ExoCarta_23204
Vesiclepedia VP_23204
Entrez Gene 23204
HGNC 697
MIM 607669
UniProt Q15041  
 ARL6IP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ARL6IP1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    positive regulation of L-glutamate import across plasma membrane GO:0002038 ISS
    cotranslational protein targeting to membrane GO:0006613 IBA
    apoptotic process GO:0006915 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    endoplasmic reticulum tubular network formation GO:0071787 IDA
    regulation of endoplasmic reticulum tubular network organization GO:1903371 IMP
    endoplasmic reticulum tubular network membrane organization GO:1990809 IDA
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    Sec61 translocon complex GO:0005784 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    cytosol GO:0005829 IEA
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    endoplasmic reticulum tubular network GO:0071782 IDA
 Experiment description of studies that identified ARL6IP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARL6IP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TM4SF5  
Affinity Capture-MS Homo sapiens
2 ZNF391  
Two-hybrid Homo sapiens
3 SENP2 59343
Two-hybrid Homo sapiens
4 C19orf18 147685
Two-hybrid Homo sapiens
5 SNX3 8724
Two-hybrid Homo sapiens
6 GPR151  
Two-hybrid Homo sapiens
7 SNX15  
Two-hybrid Homo sapiens
8 SLC10A6  
Two-hybrid Homo sapiens
9 VAPA 9218
Affinity Capture-MS Homo sapiens
10 EMP1  
Two-hybrid Homo sapiens
11 ARL6IP5 10550
Affinity Capture-MS Homo sapiens
12 CEP170 9859
Cross-Linking-MS (XL-MS) Homo sapiens
13 CTDNEP1 23399
Affinity Capture-MS Homo sapiens
14 BSCL2  
Affinity Capture-MS Homo sapiens
15 PTPN9 5780
Two-hybrid Homo sapiens
16 ZFYVE21  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 GAS8  
Two-hybrid Homo sapiens
18 GPD1L 23171
Cross-Linking-MS (XL-MS) Homo sapiens
19 ARL6IP1 23204
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 COPA 1314
Affinity Capture-MS Homo sapiens
21 RTN4 57142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
22 RNF5  
Two-hybrid Homo sapiens
23 TFAM 7019
Two-hybrid Homo sapiens
24 COPB2 9276
Affinity Capture-MS Homo sapiens
25 FBXW7  
Two-hybrid Homo sapiens
26 COL4A3BP 10087
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 NUDT3 11165
Two-hybrid Homo sapiens
28 HGS 9146
Reconstituted Complex Homo sapiens
29 HIATL1 84641
Two-hybrid Homo sapiens
30 F2RL1  
Two-hybrid Homo sapiens
31 POP1 10940
Cross-Linking-MS (XL-MS) Homo sapiens
32 ESYT2 57488
Affinity Capture-MS Homo sapiens
33 SDCBP 6386
Two-hybrid Homo sapiens
34 FAF2 23197
Affinity Capture-MS Homo sapiens
35 SNX4 8723
Two-hybrid Homo sapiens
36 MTERF3  
Two-hybrid Homo sapiens
37 TUFM 7284
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 UNK  
Affinity Capture-RNA Homo sapiens
39 MCEMP1  
Two-hybrid Homo sapiens
40 TMEM248 55069
Two-hybrid Homo sapiens
41 SPG21 51324
Two-hybrid Homo sapiens
42 SCFD1 23256
Affinity Capture-MS Homo sapiens
43 TMEM14B  
Two-hybrid Homo sapiens
44 REEP4  
Affinity Capture-MS Homo sapiens
45 IFITM3 10410
Affinity Capture-MS Homo sapiens
46 AMFR 267
Affinity Capture-MS Homo sapiens
47 ARL6 84100
Two-hybrid Homo sapiens
48 PAM16  
Two-hybrid Homo sapiens
49 DIABLO 56616
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 UBE2I 7329
Two-hybrid Homo sapiens
51 WDYHV1  
Two-hybrid Homo sapiens
52 SNX11 29916
Two-hybrid Homo sapiens
53 GORASP2 26003
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 WIPI2 26100
Two-hybrid Homo sapiens
55 TARS2 80222
Two-hybrid Homo sapiens
56 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 C12orf10 60314
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 HMGCL 3155
Two-hybrid Homo sapiens
59 VMA21  
Two-hybrid Homo sapiens
60 TSPAN31 6302
Affinity Capture-MS Homo sapiens
61 REEP5 7905
Affinity Capture-MS Homo sapiens
62 SNX1 6642
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 ATL3 25923
Affinity Capture-MS Homo sapiens
64 MFSD5  
Two-hybrid Homo sapiens
65 TMED8 283578
Two-hybrid Homo sapiens
66 FAM134A 79137
Affinity Capture-MS Homo sapiens
67 BCAP31 10134
Affinity Capture-MS Homo sapiens
68 TMEM258  
Two-hybrid Homo sapiens
69 HSD17B13  
Two-hybrid Homo sapiens
70 RIPK4  
Affinity Capture-MS Homo sapiens
71 FDFT1 2222
Two-hybrid Homo sapiens
72 GPR152  
Two-hybrid Homo sapiens
73 INPP5K 51763
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
74 NDRG4 65009
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 TSPAN8 7103
Affinity Capture-MS Homo sapiens
76 FAM134C 162427
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
77 RABAC1 10567
Affinity Capture-MS Homo sapiens
78 FXR2 9513
Two-hybrid Homo sapiens
79 ACSF2 80221
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 SNX10 29887
Two-hybrid Homo sapiens
81 FAM134B 54463
Affinity Capture-MS Homo sapiens
82 YIPF4 84272
Two-hybrid Homo sapiens
83 SHISA5 51246
Two-hybrid Homo sapiens
84 PBX3  
Two-hybrid Homo sapiens
85 REEP6  
Two-hybrid Homo sapiens
86 RAB33A 9363
Two-hybrid Homo sapiens
87 GAD2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
88 WDFY2  
Two-hybrid Homo sapiens
89 NSF 4905
Affinity Capture-MS Homo sapiens
90 TP53 7157
Affinity Capture-MS Homo sapiens
91 RTN3 10313
Affinity Capture-MS Homo sapiens
92 TRMT10A 93587
Cross-Linking-MS (XL-MS) Homo sapiens
93 ATL2 64225
Affinity Capture-MS Homo sapiens
94 GOLPH3 64083
Two-hybrid Homo sapiens
95 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
96 SUCLA2 8803
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
97 SHMT2 6472
Two-hybrid Homo sapiens
98 DDX58 23586
Affinity Capture-RNA Homo sapiens
99 NAPB 63908
Two-hybrid Homo sapiens
100 MAPK6  
Affinity Capture-MS Homo sapiens
101 GOLPH3L 55204
Two-hybrid Homo sapiens
102 RAB2A 5862
Affinity Capture-MS Homo sapiens
103 MIEF2  
Two-hybrid Homo sapiens
104 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 SNX12 29934
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
106 SNX2 6643
Two-hybrid Homo sapiens
107 RAB5C 5878
Affinity Capture-MS Homo sapiens
108 STARD4  
Two-hybrid Homo sapiens
109 ESYT1 23344
Affinity Capture-MS Homo sapiens
110 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 MGST3 4259
Two-hybrid Homo sapiens
112 SNX8 29886
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ARL6IP1 is involved
No pathways found





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