Gene description for HMGCL
Gene name 3-hydroxymethyl-3-methylglutaryl-CoA lyase
Gene symbol HMGCL
Other names/aliases HL
Species Homo sapiens
 Database cross references - HMGCL
ExoCarta ExoCarta_3155
Vesiclepedia VP_3155
Entrez Gene 3155
HGNC 5005
MIM 613898
UniProt P35914  
 HMGCL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HMGCL
Molecular Function
    magnesium ion binding GO:0000287 IDA
    hydroxymethylglutaryl-CoA lyase activity GO:0004419 IBA
    hydroxymethylglutaryl-CoA lyase activity GO:0004419 IDA
    structural molecule activity GO:0005198 IDA
    manganese ion binding GO:0030145 IDA
    metal ion binding GO:0046872 IDA
Biological Process
    L-leucine catabolic process GO:0006552 IBA
    L-leucine catabolic process GO:0006552 NAS
    L-leucine catabolic process GO:0006552 TAS
    lipid metabolic process GO:0006629 IDA
    mitochondrion organization GO:0007005 IEA
    ketone body biosynthetic process GO:0046951 IBA
    ketone body biosynthetic process GO:0046951 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 NAS
    mitochondrial matrix GO:0005759 TAS
    peroxisome GO:0005777 IDA
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified HMGCL in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HMGCL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IVD 3712
Affinity Capture-MS Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 FABP3 2170
Co-fractionation Homo sapiens
4 LSM12 124801
Co-fractionation Homo sapiens
5 GLS 2744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RNF126  
Two-hybrid Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 FPGS 2356
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MS4A7  
Two-hybrid Homo sapiens
10 NEDD4 4734
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 CTDSP1 58190
Affinity Capture-MS Homo sapiens
12 ALDH4A1 8659
Co-fractionation Homo sapiens
13 HMGCLL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MSRB2  
Co-fractionation Homo sapiens
15 PLEC 5339
Cross-Linking-MS (XL-MS) Homo sapiens
16 MCCC2 64087
Co-fractionation Homo sapiens
17 PNPT1 87178
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 MGME1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ADAMTS10 81794
Two-hybrid Homo sapiens
20 FBXO21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CYB5R3 1727
Co-fractionation Homo sapiens
22 ETFB 2109
Co-fractionation Homo sapiens
23 LYPLAL1 127018
Co-fractionation Homo sapiens
24 HSD17B10 3028
Two-hybrid Homo sapiens
25 TXNDC12 51060
Co-fractionation Homo sapiens
26 PIR 8544
Co-fractionation Homo sapiens
27 ARL6IP1 23204
Two-hybrid Homo sapiens
28 ACADVL 37
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 C21orf33  
Co-fractionation Homo sapiens
30 GTF2B 2959
Two-hybrid Homo sapiens
31 COX17  
Co-fractionation Homo sapiens
32 AKR1C2  
Co-fractionation Homo sapiens
33 FASTKD1  
Affinity Capture-MS Homo sapiens
34 GSTO1 9446
Co-fractionation Homo sapiens
35 PGM2 55276
Co-fractionation Homo sapiens
36 L2HGDH  
Affinity Capture-MS Homo sapiens
37 ALAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ACO2 50
Co-fractionation Homo sapiens
39 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 DBT 1629
Affinity Capture-MS Homo sapiens
41 ESD 2098
Co-fractionation Homo sapiens
42 RAB7A 7879
Co-fractionation Homo sapiens
43 PCCB 5096
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RAB11A 8766
Co-fractionation Homo sapiens
45 MIPEP  
Affinity Capture-MS Homo sapiens
46 CUL4A 8451
Affinity Capture-MS Homo sapiens
47 HES1 3280
Two-hybrid Homo sapiens
48 DNAJA1 3301
Two-hybrid Homo sapiens
49 ATPAF2 91647
Affinity Capture-MS Homo sapiens
50 MRPS36 92259
Affinity Capture-MS Homo sapiens
51 PEX5 5830
Protein-peptide Homo sapiens
52 ETFA 2108
Co-fractionation Homo sapiens
53 IKBKB 3551
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
54 CA2 760
Co-fractionation Homo sapiens
55 TIGAR 57103
Co-fractionation Homo sapiens
56 RAB1A 5861
Co-fractionation Homo sapiens
57 INHBA 3624
Co-fractionation Homo sapiens
58 COTL1 23406
Co-fractionation Homo sapiens
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