Gene description for INPP5K
Gene name inositol polyphosphate-5-phosphatase K
Gene symbol INPP5K
Other names/aliases PPS
SKIP
Species Homo sapiens
 Database cross references - INPP5K
ExoCarta ExoCarta_51763
Vesiclepedia VP_51763
Entrez Gene 51763
HGNC 33882
MIM 607875
UniProt Q9BT40  
 INPP5K identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for INPP5K
Molecular Function
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IBA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IMP
    vasopressin receptor activity GO:0005000 ISS
    protein binding GO:0005515 IPI
    inositol bisphosphate phosphatase activity GO:0016312 IBA
    inositol bisphosphate phosphatase activity GO:0016312 IDA
    phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity GO:0034485 IBA
    phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity GO:0034485 IDA
    phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity GO:0034485 TAS
    phosphatidylinositol trisphosphate phosphatase activity GO:0034594 IDA
    phosphatidylinositol phosphate 5-phosphatase activity GO:0034595 IMP
    lipid phosphatase activity GO:0042577 NAS
    inositol trisphosphate phosphatase activity GO:0046030 IBA
    inositol trisphosphate phosphatase activity GO:0046030 IDA
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IEA
    inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity GO:0052659 IEA
Biological Process
    in utero embryonic development GO:0001701 IEA
    negative regulation of protein phosphorylation GO:0001933 ISS
    regulation of glycogen biosynthetic process GO:0005979 ISS
    negative regulation of protein kinase activity GO:0006469 IDA
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    G protein-coupled receptor signaling pathway GO:0007186 ISS
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IDA
    negative regulation of D-glucose transmembrane transport GO:0010829 IDA
    dephosphorylation GO:0016311 IDA
    dephosphorylation GO:0016311 IMP
    actin cytoskeleton organization GO:0030036 NAS
    cellular response to insulin stimulus GO:0032869 IDA
    cellular response to hormone stimulus GO:0032870 ISS
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IDA
    negative regulation of dephosphorylation GO:0035305 ISS
    positive regulation of urine volume GO:0035810 ISS
    glucose homeostasis GO:0042593 ISS
    negative regulation of MAP kinase activity GO:0043407 IDA
    negative regulation by host of viral transcription GO:0043922 IDA
    negative regulation of glycogen biosynthetic process GO:0045719 IBA
    negative regulation of glycogen biosynthetic process GO:0045719 IDA
    negative regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045869 IDA
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of DNA-templated transcription GO:0045893 ISS
    negative regulation of insulin receptor signaling pathway GO:0046627 IBA
    negative regulation of insulin receptor signaling pathway GO:0046627 IDA
    phosphatidylinositol dephosphorylation GO:0046856 IDA
    phosphatidylinositol dephosphorylation GO:0046856 IDA
    negative regulation of stress fiber assembly GO:0051497 IDA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IBA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IDA
    negative regulation of calcium ion transport GO:0051926 IDA
    cellular response to cAMP GO:0071320 ISS
    cellular response to tumor necrosis factor GO:0071356 IDA
    cellular response to epidermal growth factor stimulus GO:0071364 IDA
    protein localization to plasma membrane GO:0072659 ISS
    negative regulation of protein targeting to membrane GO:0090315 IDA
    ruffle assembly GO:0097178 IDA
    negative regulation of glycogen (starch) synthase activity GO:2000466 ISS
    positive regulation of renal water transport GO:2001153 ISS
Subcellular Localization
    ruffle GO:0001726 IBA
    ruffle GO:0001726 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    ruffle membrane GO:0032587 IDA
    neuron projection GO:0043005 IBA
    neuron projection GO:0043005 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified INPP5K in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for INPP5K
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RFX3 5991
Affinity Capture-MS Homo sapiens
3 DOCK1 1793
Affinity Capture-MS Homo sapiens
4 P4HA1 5033
Affinity Capture-MS Homo sapiens
5 GAN 8139
Affinity Capture-MS Homo sapiens
6 TANK 10010
Two-hybrid Homo sapiens
7 FASN 2194
Negative Genetic Homo sapiens
8 SMN1 6606
Two-hybrid Homo sapiens
9 FAM20C 56975
Affinity Capture-MS Homo sapiens
10 TK1 7083
Two-hybrid Homo sapiens
11 ATP5J 522
Two-hybrid Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 PES1 23481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 SFT2D2 375035
Two-hybrid Homo sapiens
15 FATE1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 ABHD10 55347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HMOX2 3163
Two-hybrid Homo sapiens
18 TTR 7276
Two-hybrid Homo sapiens
19 ANXA7 310
Two-hybrid Homo sapiens
20 FBXL19  
Affinity Capture-MS Homo sapiens
21 GOLT1B 51026
Two-hybrid Homo sapiens
22 MTG2  
Affinity Capture-MS Homo sapiens
23 PFDN1 5201
Two-hybrid Homo sapiens
24 RPL10 6134
Affinity Capture-MS Homo sapiens
25 MAD2L1BP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HSPB1 3315
Two-hybrid Homo sapiens
27 RFX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CDKN1A  
Two-hybrid Homo sapiens
29 KATNA1 11104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ARL6IP1 23204
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 SNX6 58533
Affinity Capture-MS Homo sapiens
32 NCOR2  
Two-hybrid Homo sapiens
33 PBX3  
Two-hybrid Homo sapiens
34 NXF1 10482
Affinity Capture-RNA Homo sapiens
35 RIPK3 11035
Affinity Capture-MS Homo sapiens
36 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KRT33B 3884
Affinity Capture-MS Homo sapiens
38 MOV10 4343
Affinity Capture-RNA Homo sapiens
39 USP47 55031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 WDYHV1  
Affinity Capture-MS Homo sapiens
41 BDNF 627
Two-hybrid Homo sapiens
42 MDC1  
Affinity Capture-MS Homo sapiens
43 BRPF3  
Affinity Capture-MS Homo sapiens
44 GPHA2  
Affinity Capture-MS Homo sapiens
45 HSPD1 3329
Affinity Capture-MS Homo sapiens
46 AAMP 14
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C1orf131  
Affinity Capture-MS Homo sapiens
48 KRT31 3881
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
49 FADS6  
Two-hybrid Homo sapiens
50 PVRL3 25945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ASPM 259266
Negative Genetic Homo sapiens
52 CPTP  
Two-hybrid Homo sapiens
53 TNFRSF10D 8793
Two-hybrid Homo sapiens
54 PROSER2 254427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 GART 2618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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