Gene description for ABHD10
Gene name abhydrolase domain containing 10
Gene symbol ABHD10
Other names/aliases -
Species Homo sapiens
 Database cross references - ABHD10
ExoCarta ExoCarta_55347
Vesiclepedia VP_55347
Entrez Gene 55347
HGNC 25656
UniProt Q9NUJ1  
 ABHD10 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for ABHD10
Molecular Function
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IBA
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IDA
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 TAS
    protein binding GO:0005515 IPI
    palmitoyl-(protein) hydrolase activity GO:0008474 IBA
    palmitoyl-(protein) hydrolase activity GO:0008474 IDA
    mycophenolic acid acyl-glucuronide esterase activity GO:0102390 IEA
Biological Process
    protein depalmitoylation GO:0002084 IDA
    acyl deglucuronidation GO:0036235 IDA
    cellular glucuronidation GO:0052695 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified ABHD10 in sEVs
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ABHD10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBR4 23352
Affinity Capture-MS Homo sapiens
2 CISD3 284106
Affinity Capture-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 APOA1BP 128240
Co-fractionation Homo sapiens
5 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
6 MECP2 4204
Affinity Capture-MS Homo sapiens
7 PMPCB 9512
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
8 LARS2 23395
Affinity Capture-MS Homo sapiens
9 SOD2 6648
Co-fractionation Homo sapiens
10 UBE2A  
Affinity Capture-MS Homo sapiens
11 HADHB 3032
Affinity Capture-MS Homo sapiens
12 ZNF462  
Affinity Capture-MS Homo sapiens
13 HTRA2 27429
Co-fractionation Homo sapiens
14 FBXL6  
Proximity Label-MS Homo sapiens
15 CYP24A1  
Affinity Capture-MS Homo sapiens
16 PITRM1 10531
Co-fractionation Homo sapiens
17 MKNK1  
Affinity Capture-MS Homo sapiens
18 NFU1 27247
Co-fractionation Homo sapiens
19 SUCLG2 8801
Co-fractionation Homo sapiens
20 NME4 4833
Affinity Capture-MS Homo sapiens
21 RPA2 6118
Proximity Label-MS Homo sapiens
22 KCMF1 56888
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DLD 1738
Affinity Capture-MS Homo sapiens
24 ZZEF1 23140
Affinity Capture-MS Homo sapiens
25 PDHA1 5160
Proximity Label-MS Homo sapiens
26 NFS1 9054
Affinity Capture-MS Homo sapiens
27 SF1 7536
Affinity Capture-MS Homo sapiens
28 NDUFAF7  
Affinity Capture-MS Homo sapiens
29 SQSTM1 8878
Affinity Capture-MS Homo sapiens
30 SUCLG1 8802
Co-fractionation Homo sapiens
31 GAPDHS 26330
Affinity Capture-MS Homo sapiens
32 MCCC1 56922
Affinity Capture-MS Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 COX8A  
Proximity Label-MS Homo sapiens
35 TXNDC12 51060
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
36 SUCLA2 8803
Co-fractionation Homo sapiens
37 RMDN3 55177
Affinity Capture-MS Homo sapiens
38 C21orf33  
Proximity Label-MS Homo sapiens
39 RTN4 57142
Affinity Capture-MS Homo sapiens
40 CALM1 801
Affinity Capture-MS Homo sapiens
41 APP 351
Reconstituted Complex Homo sapiens
42 OAT 4942
Co-fractionation Homo sapiens
43 ZMAT2  
Affinity Capture-MS Homo sapiens
44 C7orf55  
Affinity Capture-MS Homo sapiens
45 FASTKD3  
Proximity Label-MS Homo sapiens
46 INPP5K 51763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 DARS2 55157
Affinity Capture-MS Homo sapiens
48 CALM2 805
Affinity Capture-MS Homo sapiens
49 UBR5 51366
Affinity Capture-MS Homo sapiens
50 KDELC2 143888
Co-fractionation Homo sapiens
51 RPUSD4 84881
Proximity Label-MS Homo sapiens
52 AARS2  
Proximity Label-MS Homo sapiens
53 UBE2B 7320
Affinity Capture-MS Homo sapiens
54 RPS10 6204
Cross-Linking-MS (XL-MS) Homo sapiens
55 PMPCA 23203
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
56 FOXK2  
Affinity Capture-MS Homo sapiens
57 D2HGDH 728294
Affinity Capture-MS Homo sapiens
58 ANXA5 308
Co-fractionation Homo sapiens
59 VWA8 23078
Affinity Capture-MS Homo sapiens
60 REXO2 25996
Co-fractionation Homo sapiens
61 ACSF3 197322
Affinity Capture-MS Homo sapiens
62 C1QBP 708
Proximity Label-MS Homo sapiens
63 YARS2  
Affinity Capture-MS Homo sapiens
64 EHMT1  
Affinity Capture-MS Homo sapiens
65 HSPBAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 MDH1 4190
Co-fractionation Homo sapiens
67 HSPD1 3329
Affinity Capture-MS Homo sapiens
68 LONP1 9361
Affinity Capture-MS Homo sapiens
69 CALM3 808
Affinity Capture-MS Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 FOXK1 221937
Affinity Capture-MS Homo sapiens
72 TULP3 7289
Affinity Capture-MS Homo sapiens
73 PAGE1  
Affinity Capture-MS Homo sapiens
74 Ubr5  
Affinity Capture-MS Mus musculus
75 H2AFX 3014
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ABHD10 is involved
PathwayEvidenceSource
Biological oxidations TAS Reactome
Glucuronidation TAS Reactome
Metabolism TAS Reactome
Phase II - Conjugation of compounds TAS Reactome





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