Gene description for MCCC1
Gene name methylcrotonoyl-CoA carboxylase 1 (alpha)
Gene symbol MCCC1
Other names/aliases MCC-B
MCCA
Species Homo sapiens
 Database cross references - MCCC1
ExoCarta ExoCarta_56922
Vesiclepedia VP_56922
Entrez Gene 56922
HGNC 6936
MIM 609010
UniProt Q96RQ3  
 MCCC1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for MCCC1
Molecular Function
    biotin carboxylase activity GO:0004075 NAS
    methylcrotonoyl-CoA carboxylase activity GO:0004485 IBA
    methylcrotonoyl-CoA carboxylase activity GO:0004485 IDA
    methylcrotonoyl-CoA carboxylase activity GO:0004485 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    biotin binding GO:0009374 NAS
    metal ion binding GO:0046872 IEA
Biological Process
    L-leucine catabolic process GO:0006552 IEA
    L-leucine catabolic process GO:0006552 ISS
    L-leucine catabolic process GO:0006552 NAS
    biotin metabolic process GO:0006768 NAS
    branched-chain amino acid catabolic process GO:0009083 NAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 NAS
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 TAS
    methylcrotonoyl-CoA carboxylase complex GO:1905202 IDA
    methylcrotonoyl-CoA carboxylase complex GO:1905202 IPI
    methylcrotonoyl-CoA carboxylase complex GO:1905202 NAS
    methylcrotonoyl-CoA carboxylase complex GO:1905202 TAS
 Experiment description of studies that identified MCCC1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCCC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
2 ISX  
Proximity Label-MS Homo sapiens
3 HOOK1  
Proximity Label-MS Homo sapiens
4 ACSM5 54988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
6 RPA2 6118
Proximity Label-MS Homo sapiens
7 LTBP2 4053
Co-fractionation Homo sapiens
8 FBXL19  
Affinity Capture-MS Homo sapiens
9 MTG2  
Affinity Capture-MS Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 KIF5B 3799
Co-fractionation Homo sapiens
12 RNF208  
Affinity Capture-MS Homo sapiens
13 FOXRED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TRMU  
Affinity Capture-MS Homo sapiens
15 PC 5091
Affinity Capture-MS Homo sapiens
16 DYRK1A 1859
Affinity Capture-MS Homo sapiens
17 DCTN4 51164
Proximity Label-MS Homo sapiens
18 MRPL23 6150
Affinity Capture-MS Homo sapiens
19 DUSP14 11072
Affinity Capture-MS Homo sapiens
20 YARS2  
Affinity Capture-MS Homo sapiens
21 MRPS24 64951
Affinity Capture-MS Homo sapiens
22 CHCHD10  
Affinity Capture-MS Homo sapiens
23 GCAT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 FTSJ2 29960
Affinity Capture-MS Homo sapiens
25 MALSU1  
Affinity Capture-MS Homo sapiens
26 METTL2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 DYNLT1 6993
Proximity Label-MS Homo sapiens
28 ADSL 158
Co-fractionation Homo sapiens
29 AGO2 27161
Affinity Capture-MS Homo sapiens
30 ACTA2 59
Affinity Capture-MS Homo sapiens
31 GATA4  
Affinity Capture-MS Homo sapiens
32 SLC25A10 1468
Affinity Capture-MS Homo sapiens
33 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
34 CUL4B 8450
Affinity Capture-MS Homo sapiens
35 TUFM 7284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DHRS2 10202
Affinity Capture-MS Homo sapiens
37 ACACA 31
Affinity Capture-MS Homo sapiens
38 PCCA 5095
Affinity Capture-MS Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 DTX2 113878
Proximity Label-MS Homo sapiens
41 DCTN1 1639
Co-fractionation Homo sapiens
42 HUWE1 10075
Affinity Capture-MS Homo sapiens
43 DDX1 1653
Co-fractionation Homo sapiens
44 HSD17B8  
Affinity Capture-MS Homo sapiens
45 VSX1  
Proximity Label-MS Homo sapiens
46 ECH1 1891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C15orf48  
Affinity Capture-MS Homo sapiens
48 SLX4  
Affinity Capture-MS Homo sapiens
49 PER2  
Proximity Label-MS Homo sapiens
50 OTC 5009
Affinity Capture-MS Homo sapiens
51 RARS2  
Affinity Capture-MS Homo sapiens
52 PNPLA4  
Affinity Capture-MS Homo sapiens
53 LHX6  
Proximity Label-MS Homo sapiens
54 NAA38  
Affinity Capture-MS Homo sapiens
55 MCCC2 64087
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 FAM83A  
Affinity Capture-MS Homo sapiens
57 UQCRC2 7385
Affinity Capture-MS Homo sapiens
58 C2orf47  
Affinity Capture-MS Homo sapiens
59 SERPINE1 5054
Co-fractionation Homo sapiens
60 HOOK3 84376
Proximity Label-MS Homo sapiens
61 HDHD3 81932
Affinity Capture-MS Homo sapiens
62 HTRA3 94031
Affinity Capture-MS Homo sapiens
63 ECI2 10455
Affinity Capture-MS Homo sapiens
64 IFIT1 3434
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 CBR4  
Affinity Capture-MS Homo sapiens
67 GALK1 2584
Affinity Capture-Luminescence Homo sapiens
68 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ATPAF2 91647
Affinity Capture-MS Homo sapiens
70 RNF2  
Affinity Capture-MS Homo sapiens
71 NPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 HIP1R 9026
Co-fractionation Homo sapiens
73 PFDN5 5204
Affinity Capture-MS Homo sapiens
74 PIGR 5284
Affinity Capture-MS Homo sapiens
75 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
76 MTHFD2 10797
Co-fractionation Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 LDHAL6B 92483
Affinity Capture-MS Homo sapiens
79 MRPS2 51116
Affinity Capture-MS Homo sapiens
80 ZNF746  
Affinity Capture-MS Homo sapiens
81 ABHD10 55347
Affinity Capture-MS Homo sapiens
82 NUDT13  
Affinity Capture-MS Homo sapiens
83 LGALS3 3958
Affinity Capture-MS Homo sapiens
84 ACAA2 10449
Affinity Capture-MS Homo sapiens
85 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SUCLA2 8803
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 ARF4 378
Proximity Label-MS Homo sapiens
88 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
89 DYNC1H1 1778
Co-fractionation Homo sapiens
90 TMEM70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 C9orf173  
Affinity Capture-MS Homo sapiens
92 COMMD5 28991
Affinity Capture-MS Homo sapiens
93 EEF1D 1936
Co-fractionation Homo sapiens
94 RPL7A 6130
Cross-Linking-MS (XL-MS) Homo sapiens
95 PCCB 5096
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 DSG4 147409
Affinity Capture-MS Homo sapiens
97 RPUSD3  
Affinity Capture-MS Homo sapiens
98 ARF1 375
Proximity Label-MS Homo sapiens
99 MYL10 93408
Affinity Capture-MS Homo sapiens
100 SOS2 6655
Affinity Capture-MS Homo sapiens
101 OXCT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 PAF1 54623
Affinity Capture-MS Homo sapiens
103 YBEY  
Affinity Capture-MS Homo sapiens
104 TRIM43  
Affinity Capture-MS Homo sapiens
105 NAP1L5  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here