Gene description for HDHD3
Gene name haloacid dehalogenase-like hydrolase domain containing 3
Gene symbol HDHD3
Other names/aliases 2810435D12Rik
C9orf158
Species Homo sapiens
 Database cross references - HDHD3
ExoCarta ExoCarta_81932
Vesiclepedia VP_81932
Entrez Gene 81932
HGNC 28171
UniProt Q9BSH5  
 HDHD3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for HDHD3
Molecular Function
    protein binding GO:0005515 IPI
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleolus GO:0005730 IDA
    mitochondrion GO:0005739 HTP
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified HDHD3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HDHD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDAH2 23564
Co-fractionation Homo sapiens
2 NDUFAB1 4706
Co-fractionation Homo sapiens
3 TRIP13 9319
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 FOXH1  
Two-hybrid Homo sapiens
5 CLIC1 1192
Co-fractionation Homo sapiens
6 PGLS 25796
Co-fractionation Homo sapiens
7 PROP1  
Two-hybrid Homo sapiens
8 MTPN 136319
Affinity Capture-MS Homo sapiens
9 DDAH1 23576
Co-fractionation Homo sapiens
10 FEN1 2237
Co-fractionation Homo sapiens
11 ATG9A 79065
Two-hybrid Homo sapiens
12 PRNP 5621
Affinity Capture-MS Homo sapiens
13 VAC14 55697
Two-hybrid Homo sapiens
14 AES 166
Two-hybrid Homo sapiens
15 LONP1 9361
Affinity Capture-MS Homo sapiens
16 GCSH 2653
Co-fractionation Homo sapiens
17 UCHL1 7345
Co-fractionation Homo sapiens
18 CMPK1 51727
Co-fractionation Homo sapiens
19 THEM4  
Co-fractionation Homo sapiens
20 ARCN1 372
Co-fractionation Homo sapiens
21 NENF 29937
Co-fractionation Homo sapiens
22 C19orf25  
Affinity Capture-MS Homo sapiens
23 PARK7 11315
Co-fractionation Homo sapiens
24 CSTF2 1478
Two-hybrid Homo sapiens
25 KLHL38  
Two-hybrid Homo sapiens
26 PGAM2 5224
Affinity Capture-MS Homo sapiens
27 MICB  
Affinity Capture-MS Homo sapiens
28 MOV10 4343
Affinity Capture-RNA Homo sapiens
29 PCCA 5095
Affinity Capture-MS Homo sapiens
30 POU4F2  
Two-hybrid Homo sapiens
31 CYSRT1 375791
Two-hybrid Homo sapiens
32 PITRM1 10531
Co-fractionation Homo sapiens
33 LACTB2 51110
Co-fractionation Homo sapiens
34 ACO2 50
Co-fractionation Homo sapiens
35 POLDIP2 26073
Co-fractionation Homo sapiens
36 CYP24A1  
Affinity Capture-MS Homo sapiens
37 HINT2 84681
Co-fractionation Homo sapiens
38 FANCL 55120
Two-hybrid Homo sapiens
39 PDP1 54704
Co-fractionation Homo sapiens
40 ACO1 48
Co-fractionation Homo sapiens
41 ADA 100
Co-fractionation Homo sapiens
42 MCCC1 56922
Affinity Capture-MS Homo sapiens
43 SMYD1  
Two-hybrid Homo sapiens
44 PCCB 5096
Affinity Capture-MS Homo sapiens
45 CLIC4 25932
Co-fractionation Homo sapiens
46 FAHD2A 51011
Co-fractionation Homo sapiens
47 FDX1L  
Co-fractionation Homo sapiens
48 PLSCR4  
Two-hybrid Homo sapiens
49 MTHFD2L 441024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HDHD3 is involved
No pathways found





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