Gene description for NENF
Gene name neudesin neurotrophic factor
Gene symbol NENF
Other names/aliases CIR2
SCIRP10
SPUF
Species Homo sapiens
 Database cross references - NENF
ExoCarta ExoCarta_29937
Vesiclepedia VP_29937
Entrez Gene 29937
HGNC 30384
MIM 611874
UniProt Q9UMX5  
 NENF identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NENF
Molecular Function
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    MAPK cascade GO:0000165 IEA
    negative regulation of appetite GO:0032099 ISS
    positive regulation of MAPK cascade GO:0043410 IEA
Subcellular Localization
    extracellular space GO:0005615 IDA
    mitochondrion GO:0005739 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 IBA
 Experiment description of studies that identified NENF in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NENF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Co-fractionation Homo sapiens
2 MPST 4357
Co-fractionation Homo sapiens
3 GSTP1 2950
Co-fractionation Homo sapiens
4 NDUFAB1 4706
Co-fractionation Homo sapiens
5 RPL3 6122
Co-fractionation Homo sapiens
6 RPS3A 6189
Co-fractionation Homo sapiens
7 NAPRT 93100
Co-fractionation Homo sapiens
8 RPSA 3921
Co-fractionation Homo sapiens
9 SELM 140606
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PRDX6 9588
Co-fractionation Homo sapiens
11 RPL32 6161
Co-fractionation Homo sapiens
12 SPTAN1 6709
Co-fractionation Homo sapiens
13 CLIC1 1192
Co-fractionation Homo sapiens
14 SOD1 6647
Co-fractionation Homo sapiens
15 MAN2B1 4125
Co-fractionation Homo sapiens
16 JUP 3728
Co-fractionation Homo sapiens
17 RPS2 6187
Co-fractionation Homo sapiens
18 DDAH1 23576
Co-fractionation Homo sapiens
19 PRNP 5621
Affinity Capture-MS Homo sapiens
20 CALR3  
Proximity Label-MS Homo sapiens
21 RPS26 6231
Co-fractionation Homo sapiens
22 PDIA4 9601
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
23 CALU 813
Proximity Label-MS Homo sapiens
24 LACTB2 51110
Co-fractionation Homo sapiens
25 GCSH 2653
Co-fractionation Homo sapiens
26 SSR4 6748
Co-fractionation Homo sapiens
27 THEM4  
Co-fractionation Homo sapiens
28 RPS8 6202
Co-fractionation Homo sapiens
29 PRPH 5630
Co-fractionation Homo sapiens
30 RPL14 9045
Co-fractionation Homo sapiens
31 PTBP3 9991
Co-fractionation Homo sapiens
32 MRPL23 6150
Co-fractionation Homo sapiens
33 TXNDC12 51060
Co-fractionation Homo sapiens
34 CLIC4 25932
Co-fractionation Homo sapiens
35 SHMT2 6472
Co-fractionation Homo sapiens
36 PARK7 11315
Co-fractionation Homo sapiens
37 C21orf33  
Co-fractionation Homo sapiens
38 HDHD3 81932
Co-fractionation Homo sapiens
39 PPT2  
Co-fractionation Homo sapiens
40 SPTBN1 6711
Co-fractionation Homo sapiens
41 HSPA5 3309
Proximity Label-MS Homo sapiens
42 ILF2 3608
Co-fractionation Homo sapiens
43 TUFM 7284
Co-fractionation Homo sapiens
44 GRHPR 9380
Co-fractionation Homo sapiens
45 IDH2 3418
Co-fractionation Homo sapiens
46 POLDIP2 26073
Co-fractionation Homo sapiens
47 PRKCDBP 112464
Co-fractionation Homo sapiens
48 HIBADH 11112
Co-fractionation Homo sapiens
49 SARS2 54938
Co-fractionation Homo sapiens
50 SPR 6697
Co-fractionation Homo sapiens
51 TPI1 7167
Co-fractionation Homo sapiens
52 MRPL49 740
Co-fractionation Homo sapiens
53 ACO2 50
Co-fractionation Homo sapiens
54 SPAG9 9043
Co-fractionation Homo sapiens
55 CDH2 1000
Co-fractionation Homo sapiens
56 DNAJC10 54431
Proximity Label-MS Homo sapiens
57 AKAP1 8165
Proximity Label-MS Homo sapiens
58 HINT2 84681
Co-fractionation Homo sapiens
59 PPA2 27068
Co-fractionation Homo sapiens
60 ENO1 2023
Co-fractionation Homo sapiens
61 SUPT5H 6829
Co-fractionation Homo sapiens
62 TXNDC5 81567
Co-fractionation Homo sapiens
63 RAB11FIP5 26056
Co-fractionation Homo sapiens
64 UQCRH 7388
Co-fractionation Homo sapiens
65 LRRC59 55379
Proximity Label-MS Homo sapiens
66 PDP1 54704
Co-fractionation Homo sapiens
67 HINT1 3094
Co-fractionation Homo sapiens
68 SND1 27044
Co-fractionation Homo sapiens
69 FAHD2A 51011
Co-fractionation Homo sapiens
70 SEC61B 10952
Proximity Label-MS Homo sapiens
71 PDIA5 10954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CPLX1 10815
Co-fractionation Homo sapiens
73 BLVRB 645
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NENF is involved
No pathways found





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