Gene description for GCSH
Gene name glycine cleavage system protein H (aminomethyl carrier)
Gene symbol GCSH
Other names/aliases GCE
NKH
Species Homo sapiens
 Database cross references - GCSH
ExoCarta ExoCarta_2653
Vesiclepedia VP_2653
Entrez Gene 2653
HGNC 4208
MIM 238330
UniProt P23434  
 GCSH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GCSH
Molecular Function
    aminomethyltransferase activity GO:0004047 TAS
    protein binding GO:0005515 IPI
Biological Process
    glycine catabolic process GO:0006546 TAS
    protein lipoylation GO:0009249 IDA
    glycine decarboxylation via glycine cleavage system GO:0019464 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial matrix GO:0005759 TAS
    glycine cleavage complex GO:0005960 IBA
 Experiment description of studies that identified GCSH in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GCSH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MED11  
Two-hybrid Homo sapiens
2 GSTP1 2950
Co-fractionation Homo sapiens
3 NDUFAB1 4706
Co-fractionation Homo sapiens
4 MAGEA6  
Two-hybrid Homo sapiens
5 PSMD5 5711
Co-fractionation Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 FDX1 2230
Co-fractionation Homo sapiens
8 GLOD4 51031
Co-fractionation Homo sapiens
9 PGLS 25796
Co-fractionation Homo sapiens
10 SOD1 6647
Co-fractionation Homo sapiens
11 CPOX 1371
Co-fractionation Homo sapiens
12 NMI  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 GPI 2821
Co-fractionation Homo sapiens
14 ACSF2 80221
Co-fractionation Homo sapiens
15 ARCN1 372
Co-fractionation Homo sapiens
16 GLDC 2731
Affinity Capture-MS Homo sapiens
17 MIS18A  
Two-hybrid Homo sapiens
18 UCHL1 7345
Co-fractionation Homo sapiens
19 CMPK1 51727
Co-fractionation Homo sapiens
20 MAGEA11  
Two-hybrid Homo sapiens
21 SUCLG1 8802
Co-fractionation Homo sapiens
22 EGFR 1956
Negative Genetic Homo sapiens
23 NENF 29937
Co-fractionation Homo sapiens
24 HOOK3 84376
Co-fractionation Homo sapiens
25 ZBTB16  
Two-hybrid Homo sapiens
26 ADPRHL2 54936
Co-fractionation Homo sapiens
27 HDHD3 81932
Co-fractionation Homo sapiens
28 MGME1  
Affinity Capture-MS Homo sapiens
29 PSMA1 5682
Co-fractionation Homo sapiens
30 MMGT1 93380
Affinity Capture-MS Homo sapiens
31 GRHPR 9380
Co-fractionation Homo sapiens
32 GSTO1 9446
Co-fractionation Homo sapiens
33 NUDT9 53343
Co-fractionation Homo sapiens
34 IDH2 3418
Co-fractionation Homo sapiens
35 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
36 CLIC1 1192
Co-fractionation Homo sapiens
37 PRDX6 9588
Co-fractionation Homo sapiens
38 ING5  
Two-hybrid Homo sapiens
39 TPI1 7167
Co-fractionation Homo sapiens
40 TXNL1 9352
Co-fractionation Homo sapiens
41 ACO2 50
Co-fractionation Homo sapiens
42 XPOT 11260
Co-fractionation Homo sapiens
43 HINT2 84681
Co-fractionation Homo sapiens
44 PSMB5 5693
Co-fractionation Homo sapiens
45 ENO1 2023
Co-fractionation Homo sapiens
46 COA7  
Co-fractionation Homo sapiens
47 PARK7 11315
Co-fractionation Homo sapiens
48 PGM1 5236
Co-fractionation Homo sapiens
49 CIDEB  
Two-hybrid Homo sapiens
50 LIAS  
Affinity Capture-MS Homo sapiens
51 PDP1 54704
Co-fractionation Homo sapiens
52 CLIC4 25932
Co-fractionation Homo sapiens
53 FEN1 2237
Co-fractionation Homo sapiens
54 FDX1L  
Co-fractionation Homo sapiens
55 SFI1 9814
Two-hybrid Homo sapiens
56 TMEM192 201931
Affinity Capture-MS Homo sapiens
57 C9orf142  
Affinity Capture-MS Homo sapiens
58 GNB2 2783
Affinity Capture-MS Homo sapiens
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