Gene description for NDUFAB1
Gene name NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa
Gene symbol NDUFAB1
Other names/aliases ACP
FASN2A
SDAP
Species Homo sapiens
 Database cross references - NDUFAB1
ExoCarta ExoCarta_4706
Vesiclepedia VP_4706
Entrez Gene 4706
HGNC 7694
MIM 603836
UniProt O14561  
 NDUFAB1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for NDUFAB1
Molecular Function
    acyl binding GO:0000035 IBA
    acyl carrier activity GO:0000036 IBA
    acyl carrier activity GO:0000036 NAS
    fatty acid binding GO:0005504 ISS
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    mitochondrial large ribosomal subunit binding GO:0140978 IDA
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    fatty acid biosynthetic process GO:0006633 NAS
    aerobic respiration GO:0009060 NAS
    protein lipoylation GO:0009249 IMP
    iron-sulfur cluster assembly GO:0016226 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    [2Fe-2S] cluster assembly GO:0044571 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 NAS
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial matrix GO:0005759 ISS
    mitochondrial membrane GO:0031966 ISS
    mitochondrial membrane GO:0031966 NAS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 ISS
    respiratory chain complex I GO:0045271 NAS
    mitochondrial [2Fe-2S] assembly complex GO:0099128 IDA
    iron-sulfur cluster assembly complex GO:1990229 NAS
 Experiment description of studies that identified NDUFAB1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NDUFAB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSTP1 2950
Co-fractionation Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 LYRM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
5 METTL17  
Proximity Label-MS Homo sapiens
6 CPOX 1371
Co-fractionation Homo sapiens
7 DDAH1 23576
Co-fractionation Homo sapiens
8 YWHAE 7531
Co-fractionation Homo sapiens
9 UCHL1 7345
Co-fractionation Homo sapiens
10 CMPK1 51727
Co-fractionation Homo sapiens
11 MRPL37 51253
Affinity Capture-MS Homo sapiens
12 ETFB 2109
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ARCN1 372
Co-fractionation Homo sapiens
14 NENF 29937
Co-fractionation Homo sapiens
15 PARK7 11315
Co-fractionation Homo sapiens
16 CCDC90B  
Proximity Label-MS Homo sapiens
17 C7orf55  
Affinity Capture-MS Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 ATPAF2 91647
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NDUFS3 4722
Affinity Capture-MS Homo sapiens
21 TFAM 7019
Proximity Label-MS Homo sapiens
22 KIF23 9493
Affinity Capture-MS Homo sapiens
23 SDHB 6390
Affinity Capture-MS Homo sapiens
24 NDUFA7 4701
Affinity Capture-MS Homo sapiens
25 MRPL23 6150
Two-hybrid Homo sapiens
26 SMYD3 64754
Two-hybrid Homo sapiens
27 HINT2 84681
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
28 PGM1 5236
Co-fractionation Homo sapiens
29 C21orf33  
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
30 ETFA 2108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 NT5C3A 51251
Two-hybrid Homo sapiens
32 MALSU1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FDX1L  
Co-fractionation Homo sapiens
34 NDUFV3 4731
Affinity Capture-MS Homo sapiens
35 LYRM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 PMPCB 9512
Proximity Label-MS Homo sapiens
37 NDUFA11 126328
Affinity Capture-MS Homo sapiens
38 ACSF2 80221
Co-fractionation Homo sapiens
39 VWA8 23078
Proximity Label-MS Homo sapiens
40 SOD1 6647
Co-fractionation Homo sapiens
41 NDUFA1 4694
Affinity Capture-MS Homo sapiens
42 FEN1 2237
Co-fractionation Homo sapiens
43 MDH2 4191
Proximity Label-MS Homo sapiens
44 OXSM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MRPS30 10884
Affinity Capture-MS Homo sapiens
46 ATP5A1 498
Affinity Capture-MS Homo sapiens
47 C6orf203  
Proximity Label-MS Homo sapiens
48 THEM4  
Co-fractionation Homo sapiens
49 SUCLG1 8802
Co-fractionation Homo sapiens
50 ADA 100
Co-fractionation Homo sapiens
51 DIABLO 56616
Co-fractionation Homo sapiens
52 NDUFAF5  
Affinity Capture-MS Homo sapiens
53 LYRM7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CCDC109B 55013
Proximity Label-MS Homo sapiens
55 GFM1 85476
Proximity Label-MS Homo sapiens
56 NOS2  
Co-fractionation Homo sapiens
57 ACTB 60
Co-fractionation Homo sapiens
58 LYRM4 57128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 MRPL11 65003
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
60 DNAJC30  
Affinity Capture-MS Homo sapiens
61 CLIC4 25932
Co-fractionation Homo sapiens
62 ACN9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 MRPL2 51069
Affinity Capture-MS Homo sapiens
64 DDAH2 23564
Co-fractionation Homo sapiens
65 SDHAF1  
Affinity Capture-MS Homo sapiens
66 GLOD4 51031
Co-fractionation Homo sapiens
67 PGLS 25796
Co-fractionation Homo sapiens
68 MTIF2 4528
Proximity Label-MS Homo sapiens
69 NDUFA9 4704
Affinity Capture-MS Homo sapiens
70 CLPP 8192
Proximity Label-MS Homo sapiens
71 MTIF3  
Proximity Label-MS Homo sapiens
72 TPI1 7167
Co-fractionation Homo sapiens
73 GCSH 2653
Co-fractionation Homo sapiens
74 NFS1 9054
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 LYRM1  
Affinity Capture-MS Homo sapiens
76 EGFR 1956
Negative Genetic Homo sapiens
77 RBM18  
Two-hybrid Homo sapiens
78 HDHD3 81932
Co-fractionation Homo sapiens
79 C12orf65  
Proximity Label-MS Homo sapiens
80 MYCN  
Affinity Capture-MS Homo sapiens
81 TACO1  
Proximity Label-MS Homo sapiens
82 MYC  
Affinity Capture-MS Homo sapiens
83 FDX1 2230
Co-fractionation Homo sapiens
84 LACTB2 51110
Co-fractionation Homo sapiens
85 TXNL1 9352
Co-fractionation Homo sapiens
86 ACAD9 28976
Proximity Label-MS Homo sapiens
87 ENO1 2023
Co-fractionation Homo sapiens
88 PDP1 54704
Co-fractionation Homo sapiens
89 METTL15  
Proximity Label-MS Homo sapiens
90 NDUFS1 4719
Co-fractionation Homo sapiens
91 MIEF1  
Cross-Linking-MS (XL-MS) Homo sapiens
92 MTERF3  
Proximity Label-MS Homo sapiens
93 CS 1431
Proximity Label-MS Homo sapiens
94 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
95 PSMB10 5699
Affinity Capture-MS Homo sapiens
96 CLIC1 1192
Co-fractionation Homo sapiens
97 TSFM 10102
Proximity Label-MS Homo sapiens
98 AUH 549
Proximity Label-MS Homo sapiens
99 GPI 2821
Co-fractionation Homo sapiens
100 GLDC 2731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 GRHPR 9380
Co-fractionation Homo sapiens
102 ADPRHL2 54936
Co-fractionation Homo sapiens
103 SPATA20  
Affinity Capture-MS Homo sapiens
104 SSBP1 6742
Proximity Label-MS Homo sapiens
105 IDH2 3418
Co-fractionation Homo sapiens
106 TBRG4 9238
Proximity Label-MS Homo sapiens
107 PRDX6 9588
Co-fractionation Homo sapiens
108 MRPL46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 ACO2 50
Co-fractionation Homo sapiens
110 METTL3  
Affinity Capture-MS Homo sapiens
111 C1QBP 708
Proximity Label-MS Homo sapiens
112 UQCRB 7381
Cross-Linking-MS (XL-MS) Homo sapiens
113 NDUFB11 54539
Affinity Capture-MS Homo sapiens
114 NDUFB3 4709
Affinity Capture-MS Homo sapiens
115 MRPL28 10573
Affinity Capture-MS Homo sapiens
116 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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