Gene description for ATP5A1
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Gene symbol ATP5A1
Other names/aliases ATP5A
ATP5AL2
ATPM
COXPD22
HEL-S-123m
MC5DN4
MOM2
OMR
ORM
hATP1
Species Homo sapiens
 Database cross references - ATP5A1
ExoCarta ExoCarta_498
Vesiclepedia VP_498
Entrez Gene 498
HGNC 823
MIM 164360
UniProt P25705  
 ATP5A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for ATP5A1
Molecular Function
    protease binding GO:0002020 IEA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 ISS
    ATP hydrolysis activity GO:0016887 IEA
    MHC class I protein binding GO:0042288 IDA
    ADP binding GO:0043531 IBA
    angiostatin binding GO:0043532 IPI
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IDA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IMP
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISS
Biological Process
    negative regulation of endothelial cell proliferation GO:0001937 IMP
    lipid metabolic process GO:0006629 ISS
    ATP biosynthetic process GO:0006754 IMP
    ATP biosynthetic process GO:0006754 NAS
    response to muscle activity GO:0014850 IEA
    proton motive force-driven ATP synthesis GO:0015986 IBA
    proton motive force-driven ATP synthesis GO:0015986 NAS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IDA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IGI
    response to ethanol GO:0045471 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    cellular response to nitric oxide GO:0071732 IEA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IC
    mitochondrion GO:0005739 NAS
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial matrix GO:0005759 TAS
    plasma membrane GO:0005886 IDA
    COP9 signalosome GO:0008180 IDA
    cell surface GO:0009986 IEA
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    membrane raft GO:0045121 IEA
    proton-transporting ATP synthase complex GO:0045259 IDA
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ATP5A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
14
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
16
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
26
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
31
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
32
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
34
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
36
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
37
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
39
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
40
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
41
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
43
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
44
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
46
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
47
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
48
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
49
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
50
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
51
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
52
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATP5A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TAS2R19  
Affinity Capture-MS Homo sapiens
2 ISG15 9636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 WDR6 11180
Co-fractionation Homo sapiens
4 UBL4A 8266
Affinity Capture-MS Homo sapiens
5 RIN3  
Affinity Capture-MS Homo sapiens
6 SKI 6497
Affinity Capture-MS Homo sapiens
7 EBNA-LP  
Affinity Capture-MS
8 TP63  
Affinity Capture-MS Homo sapiens
9 CMPK1 51727
Co-fractionation Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 UBC 7316
Affinity Capture-MS Homo sapiens
12 CSNK2A1 1457
Biochemical Activity Homo sapiens
13 HDAC4  
Affinity Capture-MS Homo sapiens
14 APEX1 328
Affinity Capture-RNA Homo sapiens
15 EFNA3  
Affinity Capture-MS Homo sapiens
16 WDR76  
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
17 PFKL 5211
Co-fractionation Homo sapiens
18 DTX3  
Affinity Capture-MS Homo sapiens
19 PMPCA 23203
Affinity Capture-MS Homo sapiens
20 PSMD12 5718
Cross-Linking-MS (XL-MS) Homo sapiens
21 POU5F1  
Affinity Capture-MS Homo sapiens
22 ETFA 2108
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
23 DYNLL1 8655
Co-fractionation Homo sapiens
24 NDUFAB1 4706
Affinity Capture-MS Homo sapiens
25 GPS1 2873
Co-fractionation Homo sapiens
26 NTSR1  
Affinity Capture-MS Homo sapiens
27 SLC25A3 5250
Co-fractionation Homo sapiens
28 KIF14 9928
Affinity Capture-MS Homo sapiens
29 UNK  
Affinity Capture-RNA Homo sapiens
30 MTCH2 23788
Co-fractionation Homo sapiens
31 CRY1  
Affinity Capture-MS Homo sapiens
32 USP11 8237
Affinity Capture-MS Homo sapiens
33 TOP2B 7155
Cross-Linking-MS (XL-MS) Homo sapiens
34 TRIM31  
Affinity Capture-MS Homo sapiens
35 CERS2 29956
Affinity Capture-MS Homo sapiens
36 ESRRB  
Affinity Capture-MS Homo sapiens
37 PABPC1 26986
Co-fractionation Homo sapiens
38 YAP1 10413
Affinity Capture-MS Homo sapiens
39 MYH9 4627
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
40 TRIM21 6737
Affinity Capture-MS Homo sapiens
41 SLX4  
Affinity Capture-MS Homo sapiens
42 FAM134A 79137
Affinity Capture-MS Homo sapiens
43 CYC1 1537
Co-fractionation Homo sapiens
44 Mapk13  
Affinity Capture-MS Mus musculus
45 B4GALT3 8703
Affinity Capture-MS Homo sapiens
46 ACTC1 70
Affinity Capture-MS Homo sapiens
47 PICALM 8301
Cross-Linking-MS (XL-MS) Homo sapiens
48 VDAC2 7417
Co-fractionation Homo sapiens
49 PAPSS2 9060
Co-fractionation Homo sapiens
50 SSR4 6748
Co-fractionation Homo sapiens
51 ANLN 54443
Affinity Capture-MS Homo sapiens
52 HSPA5 3309
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
53 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 HADHB 3032
Co-fractionation Homo sapiens
55 HADHA 3030
Co-fractionation Homo sapiens
56 APOE 348
Co-fractionation Homo sapiens
57 IQCB1  
Affinity Capture-MS Homo sapiens
58 GOLT1B 51026
Affinity Capture-MS Homo sapiens
59 NEBL 10529
Cross-Linking-MS (XL-MS) Homo sapiens
60 BCAP31 10134
Co-fractionation Homo sapiens
61 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 YWHAZ 7534
Affinity Capture-MS Homo sapiens
63 MBOAT1  
Affinity Capture-MS Homo sapiens
64 OPRL1 4987
Affinity Capture-MS Homo sapiens
65 FN1 2335
Affinity Capture-MS Homo sapiens
66 P4HB 5034
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
67 BLK 640
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 ABCC1 4363
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
70 HSPA1A 3303
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
71 ILK 3611
Affinity Capture-MS Homo sapiens
72 SCCPDH 51097
Co-fractionation Homo sapiens
73 PPT1 5538
Affinity Capture-MS Homo sapiens
74 CLPX 10845
Affinity Capture-MS Homo sapiens
75 SHBG 6462
Two-hybrid Homo sapiens
76 GRB2 2885
Co-fractionation Homo sapiens
77 ZFYVE27 118813
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 COX5A 9377
Co-fractionation Homo sapiens
79 FKBP9 11328
Co-fractionation Homo sapiens
80 HSP90AA1 3320
Co-fractionation Homo sapiens
81 TARS 6897
Cross-Linking-MS (XL-MS) Homo sapiens
82 LMAN1 3998
Co-fractionation Homo sapiens
83 SSBP1 6742
Co-fractionation Homo sapiens
84 WWP2 11060
Affinity Capture-MS Homo sapiens
85 ATP6V0C 527
Affinity Capture-MS Homo sapiens
86 EIF4B 1975
Co-fractionation Homo sapiens
87 AIMP2 7965
Affinity Capture-MS Homo sapiens
88 RAB11A 8766
Co-fractionation Homo sapiens
89 CUL7 9820
Affinity Capture-MS Homo sapiens
90 HTR1B  
Affinity Capture-MS Homo sapiens
91 LUC7L 55692
Cross-Linking-MS (XL-MS) Homo sapiens
92 PTPRF 5792
Two-hybrid Homo sapiens
93 ACOT7 11332
Affinity Capture-MS Homo sapiens
94 AFG3L2 10939
Co-fractionation Homo sapiens
95 KIF23 9493
Affinity Capture-MS Homo sapiens
96 SPRTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 MAGEA3  
Affinity Capture-MS Homo sapiens
98 HSPA6 3310
Co-fractionation Homo sapiens
99 GJA1 2697
Affinity Capture-MS Homo sapiens
100 TARDBP 23435
Affinity Capture-MS Homo sapiens
101 RPA2 6118
Proximity Label-MS Homo sapiens
102 VPRBP 9730
Co-fractionation Homo sapiens
103 ADRB2  
Affinity Capture-MS Homo sapiens
104 Atp2a2 11938
Affinity Capture-MS Mus musculus
105 PCBP2 5094
Co-fractionation Homo sapiens
106 HSP90AB1 3326
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
107 EIF4A1 1973
Co-fractionation Homo sapiens
108 CAND1 55832
Affinity Capture-MS Homo sapiens
109 RPS3 6188
Co-fractionation Homo sapiens
110 ATP5L 10632
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
111 PGK1 5230
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
112 OGDH 4967
Co-fractionation Homo sapiens
113 HSPA2 3306
Co-fractionation Homo sapiens
114 COPS6 10980
Co-fractionation Homo sapiens
115 PRC1 9055
Affinity Capture-MS Homo sapiens
116 OBSL1 23363
Affinity Capture-MS Homo sapiens
117 C1qbp 12261
Affinity Capture-MS Mus musculus
118 PLCG1 5335
Co-fractionation Homo sapiens
119 COPS7A 50813
Co-fractionation Homo sapiens
120 VCAM1 7412
Affinity Capture-MS Homo sapiens
121 CUL1 8454
Affinity Capture-MS Homo sapiens
122 PMPCB 9512
Affinity Capture-MS Homo sapiens
123 ATP5I 521
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
124 PRNP 5621
Affinity Capture-MS Homo sapiens
125 YWHAB 7529
Affinity Capture-MS Homo sapiens
126 HNRNPA0 10949
Co-fractionation Homo sapiens
127 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
128 PSMD14 10213
Affinity Capture-MS Homo sapiens
129 PILRB 29990
Affinity Capture-MS Homo sapiens
130 TUBA1C 84790
Affinity Capture-MS Homo sapiens
131 RLIM 51132
Affinity Capture-MS Homo sapiens
132 POLDIP2 26073
Affinity Capture-MS Homo sapiens
133 UQCRC1 7384
Co-fractionation Homo sapiens
134 COPS7B 64708
Co-fractionation Homo sapiens
135 AGPS 8540
Co-fractionation Homo sapiens
136 CYLD  
Affinity Capture-MS Homo sapiens
137 PTPN11 5781
Co-fractionation Homo sapiens
138 PSMA3 5684
Affinity Capture-MS Homo sapiens
139 ACKR3 57007
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 F2RL1  
Affinity Capture-MS Homo sapiens
141 RPN2 6185
Co-fractionation Homo sapiens
142 CANX 821
Co-fractionation Homo sapiens
143 ATL3 25923
Affinity Capture-MS Homo sapiens
144 ICAM1 3383
Co-fractionation Homo sapiens
145 RC3H2  
Affinity Capture-MS Homo sapiens
146 PPA1 5464
Co-fractionation Homo sapiens
147 ATP5F1 515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
148 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 HSD17B10 3028
Two-hybrid Homo sapiens
150 FUS 2521
Affinity Capture-MS Homo sapiens
151 RIPK4  
Affinity Capture-MS Homo sapiens
152 CDK2 1017
Affinity Capture-MS Homo sapiens
153 ATP5J2 9551
Co-fractionation Homo sapiens
154 NRD1 4898
Affinity Capture-MS Homo sapiens
155 ERG  
Affinity Capture-MS Homo sapiens
156 ATP5B 506
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
157 HPRT1 3251
Co-fractionation Homo sapiens
158 RCN2 5955
Co-fractionation Homo sapiens
159 DDRGK1 65992
Affinity Capture-MS Homo sapiens
160 ABCA2 20
Affinity Capture-MS Homo sapiens
161 XRCC3  
Affinity Capture-MS Homo sapiens
162 SIRT3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
163 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
164 PHGDH 26227
Affinity Capture-MS Homo sapiens
165 EEF1A1 1915
Co-fractionation Homo sapiens
166 CLPP 8192
Proximity Label-MS Homo sapiens
167 CS 1431
Proximity Label-MS Homo sapiens
168 PSMB10 5699
Affinity Capture-MS Homo sapiens
169 FDX1 2230
Co-fractionation Homo sapiens
170 VDAC3 7419
Co-fractionation Homo sapiens
171 PARP1 142
Affinity Capture-MS Homo sapiens
172 TP53 7157
Affinity Capture-MS Homo sapiens
173 BCKDHA 593
Affinity Capture-MS Homo sapiens
174 PTBP1 5725
Co-fractionation Homo sapiens
175 COX8A  
Proximity Label-MS Homo sapiens
176 MAP2K1 5604
Affinity Capture-MS Homo sapiens
177 SLC9A3R1 9368
Co-fractionation Homo sapiens
178 C1QBP 708
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 KRAS 3845
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
180 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
181 PRMT1 3276
Affinity Capture-MS Homo sapiens
182 Smn1 20595
Affinity Capture-MS Mus musculus
183 RANBP2 5903
Cross-Linking-MS (XL-MS) Homo sapiens
184 GLRX5 51218
Affinity Capture-MS Homo sapiens
185 ATP6V1B2 526
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
186 UBE2H 7328
Affinity Capture-MS Homo sapiens
187 MTCH1 23787
Co-fractionation Homo sapiens
188 RAB1A 5861
Co-fractionation Homo sapiens
189 YWHAE 7531
Affinity Capture-MS Homo sapiens
190 CD4 920
Affinity Capture-MS Homo sapiens
191 UQCRB 7381
Co-fractionation Homo sapiens
192 SSB 6741
Co-fractionation Homo sapiens
193 RPS6KB2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
194 CAPZB 832
Affinity Capture-MS Homo sapiens
195 Kcnk1  
Affinity Capture-MS Mus musculus
196 MCM2 4171
Affinity Capture-MS Homo sapiens
197 P2RY1  
Affinity Capture-MS Homo sapiens
198 CHCHD2  
Affinity Capture-MS Homo sapiens
199 MDH2 4191
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
200 Yap1  
Reconstituted Complex Mus musculus
201 ATG16L1 55054
Affinity Capture-MS Homo sapiens
202 TFCP2 7024
Affinity Capture-MS Homo sapiens
203 NCSTN 23385
Affinity Capture-MS Homo sapiens
204 HSPA8 3312
Affinity Capture-MS Homo sapiens
205 INSIG2  
Affinity Capture-MS Homo sapiens
206 ATP5A1 498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
207 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
208 IGF1R 3480
Affinity Capture-MS Homo sapiens
209 PAM16  
Co-fractionation Homo sapiens
210 ACACA 31
Positive Genetic Homo sapiens
211 PLOD2 5352
Co-fractionation Homo sapiens
212 CHMP4B 128866
Affinity Capture-MS Homo sapiens
213 BTF3 689
Affinity Capture-MS Homo sapiens
214 NTRK1 4914
Affinity Capture-MS Homo sapiens
215 DNAJA1 3301
Cross-Linking-MS (XL-MS) Homo sapiens
216 RPN1 6184
Co-fractionation Homo sapiens
217 CTSB 1508
Co-fractionation Homo sapiens
218 HNRNPA3 220988
Co-fractionation Homo sapiens
219 HDAC5 10014
Affinity Capture-MS Homo sapiens
220 CA9 768
Affinity Capture-MS Homo sapiens
221 ECH1 1891
Affinity Capture-MS Homo sapiens
222 OGT 8473
Reconstituted Complex Homo sapiens
223 EFNA4  
Affinity Capture-MS Homo sapiens
224 AHCY 191
Co-fractionation Homo sapiens
225 METTL14  
Affinity Capture-MS Homo sapiens
226 ECT2 1894
Affinity Capture-MS Homo sapiens
227 FAM60A  
Affinity Capture-MS Homo sapiens
228 NR3C1 2908
Affinity Capture-MS Homo sapiens
229 FBXO6 26270
Affinity Capture-MS Homo sapiens
230 YIPF5 81555
Affinity Capture-MS Homo sapiens
231 NPSR1  
Two-hybrid Homo sapiens
232 NCKIPSD 51517
Co-fractionation Homo sapiens
233 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
234 GPR21  
Affinity Capture-MS Homo sapiens
235 ATPAF2 91647
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
236 MATR3 9782
Co-fractionation Homo sapiens
237 ACTB 60
Co-fractionation Homo sapiens
238 RPL18 6141
Co-fractionation Homo sapiens
239 HSPD1 3329
Co-fractionation Homo sapiens
240 HSPA9 3313
Co-fractionation Homo sapiens
241 COPS3 8533
Co-fractionation Homo sapiens
242 FLOT1 10211
Co-fractionation Homo sapiens
243 IMMT 10989
Affinity Capture-MS Homo sapiens
244 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
245 PHB2 11331
Co-fractionation Homo sapiens
246 TACR1  
Affinity Capture-MS Homo sapiens
247 ATP5J 522
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
248 ICT1 3396
Affinity Capture-MS Homo sapiens
249 MCM5 4174
Affinity Capture-MS Homo sapiens
250 PDHA1 5160
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
251 HSPB1 3315
Co-fractionation Homo sapiens
252 GNB1 2782
Co-fractionation Homo sapiens
253 PLK1 5347
Affinity Capture-MS Homo sapiens
254 TRIM25 7706
Co-fractionation Homo sapiens
255 UFL1 23376
Affinity Capture-MS Homo sapiens
256 MED12  
Affinity Capture-MS Homo sapiens
257 AARS2  
Proximity Label-MS Homo sapiens
258 RAB7A 7879
Co-fractionation Homo sapiens
259 CIT 11113
Affinity Capture-MS Homo sapiens
260 METTL3  
Affinity Capture-MS Homo sapiens
261 SERBP1 26135
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
262 AR 367
Affinity Capture-MS Homo sapiens
263 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
264 ATXN3 4287
Affinity Capture-MS Homo sapiens
265 COPS4 51138
Co-fractionation Homo sapiens
266 UBA52 7311
Affinity Capture-MS Homo sapiens
267 YME1L1 10730
Co-fractionation Homo sapiens
268 CRBN  
Affinity Capture-MS Homo sapiens
269 RIT1 6016
Negative Genetic Homo sapiens
270 SCN2A  
Cross-Linking-MS (XL-MS) Homo sapiens
271 C7orf55-LUC7L2  
Affinity Capture-MS Homo sapiens
272 ATPIF1 93974
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
273 COIL  
Proximity Label-MS Homo sapiens
274 CPOX 1371
Co-fractionation Homo sapiens
275 DDAH1 23576
Affinity Capture-MS Homo sapiens
276 FBXO25  
Affinity Capture-MS Homo sapiens
277 HSPA1L 3305
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
278 CCT4 10575
Co-fractionation Homo sapiens
279 BASP1 10409
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
280 ELAVL1 1994
Co-fractionation Homo sapiens
281 MLH1 4292
Affinity Capture-MS Homo sapiens
282 APP 351
Reconstituted Complex Homo sapiens
283 USP36  
Affinity Capture-MS Homo sapiens
284 KIAA1429 25962
Affinity Capture-MS Homo sapiens
285 KIF20A 10112
Affinity Capture-MS Homo sapiens
286 TSPO 706
Co-fractionation Homo sapiens
287 ATP5D 513
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
288 PRDX3 10935
Co-fractionation Homo sapiens
289 TGFB1 7040
Affinity Capture-MS Homo sapiens
290 CYB5R1 51706
Co-fractionation Homo sapiens
291 COPS5 10987
Co-fractionation Homo sapiens
292 FBXW7  
Affinity Capture-MS Homo sapiens
293 YWHAG 7532
Affinity Capture-MS Homo sapiens
294 DNAJC21  
Cross-Linking-MS (XL-MS) Homo sapiens
295 RER1 11079
Co-fractionation Homo sapiens
296 ATP5O 539
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
297 ATP6V0D1 9114
Co-fractionation Homo sapiens
298 CDC73  
Affinity Capture-MS Homo sapiens
299 ATP5H 10476
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
300 MYCN  
Affinity Capture-MS Homo sapiens
301 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
302 TMEM63B 55362
Affinity Capture-MS Homo sapiens
303 STOML2 30968
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
304 LRRC59 55379
Co-fractionation Homo sapiens
305 NDUFS4 4724
Co-fractionation Homo sapiens
306 PSMD4 5710
Affinity Capture-MS Homo sapiens
307 ERBB3 2065
Affinity Capture-MS Homo sapiens
308 STAU1 6780
Affinity Capture-MS Homo sapiens
309 GPR35  
Affinity Capture-MS Homo sapiens
310 RNF146  
Affinity Capture-MS Homo sapiens
311 USMG5 84833
Affinity Capture-MS Homo sapiens
312 VCP 7415
Affinity Capture-MS Homo sapiens
313 IFI16 3428
Affinity Capture-MS Homo sapiens
314 BTK 695
Affinity Capture-MS Homo sapiens
315 ZC3H15 55854
Cross-Linking-MS (XL-MS) Homo sapiens
316 RPS15A 6210
Co-fractionation Homo sapiens
317 NIPSNAP1 8508
Co-fractionation Homo sapiens
318 SLC33A1 9197
Affinity Capture-MS Homo sapiens
319 PHB 5245
Co-fractionation Homo sapiens
320 SLC25A24 29957
Co-fractionation Homo sapiens
321 COX5B 1329
Co-fractionation Homo sapiens
322 COPS2 9318
Co-fractionation Homo sapiens
323 FANCD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
324 FBXL7  
Affinity Capture-MS Homo sapiens
325 MRPL46  
Affinity Capture-MS Homo sapiens
326 NDUFB10 4716
Co-fractionation Homo sapiens
327 PINK1  
Affinity Capture-MS Homo sapiens
328 DOCK7 85440
Co-fractionation Homo sapiens
329 UQCRC2 7385
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
330 CTNNB1 1499
Affinity Capture-MS Homo sapiens
331 Bmpr1a  
Affinity Capture-MS Mus musculus
332 COPS8 10920
Co-fractionation Homo sapiens
333 NCDN 23154
Affinity Capture-MS Homo sapiens
334 NXF1 10482
Affinity Capture-RNA Homo sapiens
335 AURKB 9212
Affinity Capture-MS Homo sapiens
336 MAPK6  
Affinity Capture-MS Homo sapiens
337 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
338 ATP5E 514
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
339 ATP6V1A 523
Co-fractionation Homo sapiens
340 VDAC1 7416
Co-fractionation Homo sapiens
341 ATP5C1 509
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
342 HNRNPU 3192
Co-fractionation Homo sapiens
343 CCDC8  
Affinity Capture-MS Homo sapiens
344 HSPB2  
Two-hybrid Homo sapiens
345 RRBP1 6238
Co-fractionation Homo sapiens
346 ZNF746  
Affinity Capture-MS Homo sapiens
347 CUL5 8065
Affinity Capture-MS Homo sapiens
348 GPX4 2879
Co-fractionation Homo sapiens
349 Ckap5 75786
Affinity Capture-MS Mus musculus
350 PDHB 5162
Co-fractionation Homo sapiens
351 OPA3 80207
Affinity Capture-MS Homo sapiens
352 EPB41L2 2037
Co-fractionation Homo sapiens
353 C21orf33  
Proximity Label-MS Homo sapiens
354 MAP7D3 79649
Cross-Linking-MS (XL-MS) Homo sapiens
355 SFXN1 94081
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
356 IDE 3416
Affinity Capture-MS Homo sapiens
357 HMGB3 3149
Cross-Linking-MS (XL-MS) Homo sapiens
358 MOV10 4343
Affinity Capture-RNA Homo sapiens
359 SLC25A4 291
Co-fractionation Homo sapiens
360 MICAL3 57553
Cross-Linking-MS (XL-MS) Homo sapiens
361 ARRB1 408
Affinity Capture-MS Homo sapiens
362 TRIP4 9325
Affinity Capture-MS Homo sapiens
363 PXN 5829
Co-fractionation Homo sapiens
364 FBP1 2203
Co-fractionation Homo sapiens
365 CYB5R3 1727
Co-fractionation Homo sapiens
366 VIPR1  
Affinity Capture-MS Homo sapiens
367 RARS 5917
Co-fractionation Homo sapiens
368 C9orf72  
Affinity Capture-MS Homo sapiens
369 WARS 7453
Co-fractionation Homo sapiens
370 PTPN18 26469
Affinity Capture-MS Homo sapiens
371 CNDP2 55748
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here