Gene description for UQCRB
Gene name ubiquinol-cytochrome c reductase binding protein
Gene symbol UQCRB
Other names/aliases MC3DN3
QCR7
QP-C
QPC
UQBC
UQBP
UQCR6
UQPC
Species Homo sapiens
 Database cross references - UQCRB
ExoCarta ExoCarta_7381
Vesiclepedia VP_7381
Entrez Gene 7381
HGNC 12582
MIM 191330
UniProt P14927  
 UQCRB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for UQCRB
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    oxidative phosphorylation GO:0006119 TAS
    mitochondrial electron transport, ubiquinol to cytochrome c GO:0006122 IBA
    mitochondrial electron transport, ubiquinol to cytochrome c GO:0006122 NAS
    aerobic respiration GO:0009060 TAS
    cellular respiration GO:0045333 NAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    respiratory chain complex III GO:0045275 IBA
    respiratory chain complex GO:0098803 TAS
 Experiment description of studies that identified UQCRB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UQCRB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
3 SPECC1L 23384
Affinity Capture-MS Homo sapiens
4 NDUFS1 4719
Co-fractionation Homo sapiens
5 MAGEA1  
Two-hybrid Homo sapiens
6 HSPA4 3308
Cross-Linking-MS (XL-MS) Homo sapiens
7 RPL14 9045
Co-fractionation Homo sapiens
8 NDUFA12 55967
Co-fractionation Homo sapiens
9 CALM1 801
Affinity Capture-MS Homo sapiens
10 UQCR10 29796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NDUFA4 4697
Affinity Capture-MS Homo sapiens
12 APP 351
Co-fractionation Homo sapiens
13 SLC25A24 29957
Co-fractionation Homo sapiens
14 KIAA1429 25962
Affinity Capture-MS Homo sapiens
15 HEATR3 55027
Affinity Capture-MS Homo sapiens
16 NDUFS3 4722
Affinity Capture-MS Homo sapiens
17 NDUFB8 4714
Co-fractionation Homo sapiens
18 SDHB 6390
Co-fractionation Homo sapiens
19 COX7A2L 9167
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PTPMT1 114971
Affinity Capture-MS Homo sapiens
21 AP2A1 160
Affinity Capture-MS Homo sapiens
22 GNB1 2782
Affinity Capture-MS Homo sapiens
23 CALD1 800
Affinity Capture-MS Homo sapiens
24 DDOST 1650
Co-fractionation Homo sapiens
25 COX7B2  
Affinity Capture-MS Homo sapiens
26 VDAC1 7416
Co-fractionation Homo sapiens
27 NDUFV1 4723
Co-fractionation Homo sapiens
28 PHB 5245
Co-fractionation Homo sapiens
29 NDUFAB1 4706
Cross-Linking-MS (XL-MS) Homo sapiens
30 ATP5H 10476
Co-fractionation Homo sapiens
31 NAT8B  
Affinity Capture-MS Homo sapiens
32 NDUFA11 126328
Affinity Capture-MS Homo sapiens
33 COX1 4512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 VAPA 9218
Co-fractionation Homo sapiens
35 UQCRQ 27089
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
36 PTGES2 80142
Affinity Capture-MS Homo sapiens
37 MYO18A 399687
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ATP5A1 498
Co-fractionation Homo sapiens
39 NDUFS4 4724
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
40 RPL9 6133
Co-fractionation Homo sapiens
41 RPS10 6204
Co-fractionation Homo sapiens
42 NDUFA8 4702
Co-fractionation Homo sapiens
43 CYC1 1537
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 UQCRC1 7384
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ACTB 60
Affinity Capture-MS Homo sapiens
46 CEP76  
Affinity Capture-MS Homo sapiens
47 UQCRH 7388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MYL12A 10627
Co-fractionation Homo sapiens
49 TMEM70  
Proximity Label-MS Homo sapiens
50 COX4I1 1327
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
51 RPL11 6135
Co-fractionation Homo sapiens
52 CISD3 284106
Affinity Capture-MS Homo sapiens
53 C15orf48  
Affinity Capture-MS Homo sapiens
54 HSPA5 3309
Co-fractionation Homo sapiens
55 ACAT1 38
Co-fractionation Homo sapiens
56 ACTBL2 345651
Affinity Capture-MS Homo sapiens
57 PRKCA 5578
Affinity Capture-MS Homo sapiens
58 RPS2 6187
Co-fractionation Homo sapiens
59 SDHA 6389
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
60 UQCRC2 7385
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
61 ACCS  
Two-hybrid Homo sapiens
62 CTNNB1 1499
Affinity Capture-MS Homo sapiens
63 MAGEB4  
Two-hybrid Homo sapiens
64 TJP1 7082
Affinity Capture-MS Homo sapiens
65 ASS1 445
Affinity Capture-MS Homo sapiens
66 ATP5F1 515
Co-fractionation Homo sapiens
67 COX6A1  
Affinity Capture-MS Homo sapiens
68 HDHD2 84064
Affinity Capture-MS Homo sapiens
69 EGFR 1956
Negative Genetic Homo sapiens
70 ATP1B3 483
Co-fractionation Homo sapiens
71 FKBP5 2289
Affinity Capture-MS Homo sapiens
72 APOOL 139322
Affinity Capture-MS Homo sapiens
73 COX6B1 1340
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 ESD 2098
Affinity Capture-MS Homo sapiens
75 MAGEA4 4103
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
76 SLC25A17 10478
Co-fractionation Homo sapiens
77 ATP1B1 481
Co-fractionation Homo sapiens
78 NDUFA10 4705
Co-fractionation Homo sapiens
79 CLTC 1213
Affinity Capture-MS Homo sapiens
80 CYTB 4519
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 UHRF1 29128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 HPCA 3208
Two-hybrid Homo sapiens
83 PHGDH 26227
Affinity Capture-MS Homo sapiens
84 RPS16 6217
Co-fractionation Homo sapiens
85 CLPP 8192
Proximity Label-MS Homo sapiens
86 CS 1431
Proximity Label-MS Homo sapiens
87 PSMB10 5699
Affinity Capture-MS Homo sapiens
88 RPL6 6128
Co-fractionation Homo sapiens
89 COX4I2 84701
Affinity Capture-MS Homo sapiens
90 LARS2 23395
Affinity Capture-MS Homo sapiens
91 ALDH1B1 219
Co-fractionation Homo sapiens
92 COX5A 9377
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
93 TIMM10  
Co-fractionation Homo sapiens
94 S100A10 6281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 HSPB1 3315
Two-hybrid Homo sapiens
96 PDHB 5162
Co-fractionation Homo sapiens
97 RABL6 55684
Affinity Capture-MS Homo sapiens
98 AARS2  
Proximity Label-MS Homo sapiens
99 RPL23 9349
Co-fractionation Homo sapiens
100 ZSWIM7  
Two-hybrid Homo sapiens
101 KRAS 3845
Negative Genetic Homo sapiens
102 COX7C 1350
Affinity Capture-MS Homo sapiens
103 NDUFB11 54539
Co-fractionation Homo sapiens
104 C17orf59 54785
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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