Experiment description of studies that identified HPCA in sEVs

1 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for HPCA

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GPM6A 2823 Affinity Capture-MS Homo sapiens 2 C1QTNF2   Two-hybrid Homo sapiens 3 DNAAF2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 AP2B1 163 Two-hybrid Homo sapiens 5 PRR35   Two-hybrid Homo sapiens 6 TFRC 7037 Affinity Capture-Western Homo sapiens 7 KIAA1804   Affinity Capture-MS Homo sapiens 8 TP53 7157 Affinity Capture-MS Homo sapiens 9 NCALD 83988 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Affinity Capture-MS Homo sapiens 10 HPCAL1 3241 Cross-Linking-MS (XL-MS) Homo sapiens Affinity Capture-MS Homo sapiens 11 GATA5   Two-hybrid Homo sapiens 12 GRM2 2912 Affinity Capture-Western Homo sapiens 13 CST3 1471 Affinity Capture-MS Homo sapiens 14 UQCRB 7381 Two-hybrid Homo sapiens 15 IL36RN   Two-hybrid Homo sapiens 16 NAIP   Reconstituted Complex Homo sapiens 17 VWC2   Two-hybrid Homo sapiens 18 MAP3K9   Affinity Capture-MS Homo sapiens 19 TXNRD3NB   Affinity Capture-MS Homo sapiens 20 SLC16A3 9123 Two-hybrid Homo sapiens 21 NMT1 4836 Affinity Capture-MS Homo sapiens 22 ABHD6 57406 Affinity Capture-MS Homo sapiens 23 CKMT1A 548596 Two-hybrid Homo sapiens 24 DGUOK   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 DTX2 113878 Affinity Capture-MS Homo sapiens 26 NUFIP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 NMT2 9397 Affinity Capture-MS Homo sapiens 28 TPP2 7174 Affinity Capture-MS Homo sapiens 29 PUSL1   Affinity Capture-MS Homo sapiens 30 HAX1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 DHDDS   Affinity Capture-MS Homo sapiens 32 ANKRD33B   Affinity Capture-MS Homo sapiens 33 ENTHD2   Two-hybrid Homo sapiens 34 MAP3K10   Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 35 CKMT1B 1159 Two-hybrid Homo sapiens View the network

Pathways in which HPCA is involved

No pathways found