Gene description for CKMT1B
Gene name creatine kinase, mitochondrial 1B
Gene symbol CKMT1B
Other names/aliases CKMT
CKMT1
UMTCK
Species Homo sapiens
 Database cross references - CKMT1B
ExoCarta ExoCarta_1159
Vesiclepedia VP_1159
Entrez Gene 1159
HGNC 1995
MIM 123290
UniProt P12532  
 CKMT1B identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for CKMT1B
Molecular Function
    creatine kinase activity GO:0004111 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    phosphocreatine biosynthetic process GO:0046314 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified CKMT1B in sEVs
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CKMT1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Co-fractionation Homo sapiens
2 HSPE1 3336
Co-fractionation Homo sapiens
3 SUMF2 25870
Co-fractionation Homo sapiens
4 FGD5  
Affinity Capture-MS Homo sapiens
5 C9orf72  
Affinity Capture-MS Homo sapiens
6 COPS7B 64708
Affinity Capture-MS Homo sapiens
7 NUTF2 10204
Two-hybrid Homo sapiens
8 CTNNA3  
Two-hybrid Homo sapiens
9 ARHGEF15  
Affinity Capture-MS Homo sapiens
10 PSMC3 5702
Two-hybrid Homo sapiens
11 CKM  
Two-hybrid Homo sapiens
12 LCORL  
Affinity Capture-MS Homo sapiens
13 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
14 CTBP2 1488
Affinity Capture-MS Homo sapiens
15 APOO 79135
Proximity Label-MS Homo sapiens
16 TPM2 7169
Co-fractionation Homo sapiens
17 CRIP1 1396
Co-fractionation Homo sapiens
18 IQCB1  
Affinity Capture-MS Homo sapiens
19 CD274 29126
Affinity Capture-MS Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 ARHGEF39 84904
Affinity Capture-MS Homo sapiens
22 EPHX1 2052
Co-fractionation Homo sapiens
23 CA2 760
Affinity Capture-MS Homo sapiens
24 GPD1L 23171
Co-fractionation Homo sapiens
25 CALM1 801
Reconstituted Complex Homo sapiens
26 FUBP3 8939
Co-fractionation Homo sapiens
27 PPP1R16A 84988
Two-hybrid Homo sapiens
28 OCIAD1 54940
Proximity Label-MS Homo sapiens
29 DIABLO 56616
Proximity Label-MS Homo sapiens
30 HECW2  
Affinity Capture-MS Homo sapiens
31 ALDOC 230
Co-fractionation Homo sapiens
32 EPB41 2035
Affinity Capture-MS Homo sapiens
33 NTRK1 4914
Affinity Capture-MS Homo sapiens
34 C19orf52  
Proximity Label-MS Homo sapiens
35 SLC25A51  
Proximity Label-MS Homo sapiens
36 GALK1 2584
Co-fractionation Homo sapiens
37 OPA1 4976
Proximity Label-MS Homo sapiens
38 RXRG  
Two-hybrid Homo sapiens
39 Arhgef2  
Affinity Capture-MS Mus musculus
40 ARHGAP36  
Affinity Capture-MS Homo sapiens
41 SLC25A12 8604
Proximity Label-MS Homo sapiens
42 BMI1  
Affinity Capture-MS Homo sapiens
43 PGAM1 5223
Co-fractionation Homo sapiens
44 ASB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
45 OCM  
Two-hybrid Homo sapiens
46 HPCA 3208
Two-hybrid Homo sapiens
47 SURF1  
Proximity Label-MS Homo sapiens
48 HDAC5 10014
Affinity Capture-MS Homo sapiens
49 DNM1L 10059
Affinity Capture-MS Homo sapiens
50 ALDOA 226
Co-fractionation Homo sapiens
51 TPM3 7170
Co-fractionation Homo sapiens
52 CLPB 81570
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CKMT1B is involved
PathwayEvidenceSource
Creatine metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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