Gene description for PSMB10
Gene name proteasome (prosome, macropain) subunit, beta type, 10
Gene symbol PSMB10
Other names/aliases LMP10
MECL1
beta2i
Species Homo sapiens
 Database cross references - PSMB10
ExoCarta ExoCarta_5699
Vesiclepedia VP_5699
Entrez Gene 5699
HGNC 9538
MIM 176847
UniProt P40306  
 PSMB10 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Prostate cancer cells 22723089    
Thymus 23844026    
 Gene ontology annotations for PSMB10
Molecular Function
    endopeptidase activity GO:0004175 IBA
    threonine-type endopeptidase activity GO:0004298 IEA
    protein binding GO:0005515 IPI
Biological Process
    cell morphogenesis GO:0000902 IEA
    humoral immune response GO:0006959 TAS
    T cell proliferation GO:0042098 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    proteasome complex GO:0000502 TAS
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    proteasome core complex GO:0005839 ISS
    proteasome core complex, beta-subunit complex GO:0019774 IBA
    proteasome core complex, beta-subunit complex GO:0019774 ISS
    spermatoproteasome complex GO:1990111 ISS
 Experiment description of studies that identified PSMB10 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 141
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PSMB10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMB9 5698
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SGCB 6443
Affinity Capture-MS Homo sapiens
3 DDX46 9879
Affinity Capture-MS Homo sapiens
4 NDUFAB1 4706
Affinity Capture-MS Homo sapiens
5 NUP85 79902
Affinity Capture-MS Homo sapiens
6 PSME2 5721
Affinity Capture-Western Homo sapiens
7 PTN  
Two-hybrid Homo sapiens
8 HSPD1 3329
Affinity Capture-MS Homo sapiens
9 CCNDBP1 23582
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 ACAT1 38
Affinity Capture-MS Homo sapiens
11 PSMA3 5684
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
12 SLC25A6 293
Affinity Capture-MS Homo sapiens
13 PSMB3 5691
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
14 SLC25A3 5250
Affinity Capture-MS Homo sapiens
15 PSMD6 9861
Co-fractionation Homo sapiens
16 SFXN1 94081
Affinity Capture-MS Homo sapiens
17 AZGP1 563
Affinity Capture-MS Homo sapiens
18 U2SURP 23350
Affinity Capture-MS Homo sapiens
19 MDK 4192
Affinity Capture-MS Homo sapiens
20 HIGD1A 25994
Affinity Capture-MS Homo sapiens
21 ACTR2 10097
Affinity Capture-MS Homo sapiens
22 STOML2 30968
Affinity Capture-MS Homo sapiens
23 FLII 2314
Affinity Capture-MS Homo sapiens
24 PSMD5 5711
Co-fractionation Homo sapiens
25 MYH14 79784
Affinity Capture-MS Homo sapiens
26 ANXA7 310
Two-hybrid Homo sapiens
27 CD2AP 23607
Affinity Capture-MS Homo sapiens
28 ZFP64  
Affinity Capture-MS Homo sapiens
29 POMP  
Two-hybrid Homo sapiens
30 ECD  
Affinity Capture-MS Homo sapiens
31 PSMB4 5692
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
32 UQCRB 7381
Affinity Capture-MS Homo sapiens
33 PSMA2 5683
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
34 UQCRC1 7384
Affinity Capture-MS Homo sapiens
35 PSMD4 5710
Co-fractionation Homo sapiens
36 ITPR3 3710
Affinity Capture-MS Homo sapiens
37 ITPRIP  
Affinity Capture-MS Homo sapiens
38 SRSF2 6427
Affinity Capture-MS Homo sapiens
39 BCCIP 56647
Affinity Capture-MS Homo sapiens
40 CHERP 10523
Affinity Capture-MS Homo sapiens
41 PSME1 5720
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
42 LPGAT1 9926
Affinity Capture-MS Homo sapiens
43 PSMB8 5696
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
44 UNC45A 55898
Co-fractionation Homo sapiens
45 PSMA1 5682
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
46 GLUD1 2746
Affinity Capture-MS Homo sapiens
47 CRX  
Two-hybrid Homo sapiens
48 TMOD1  
Affinity Capture-MS Homo sapiens
49 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
50 TPM1 7168
Affinity Capture-MS Homo sapiens
51 AIFM1 9131
Affinity Capture-MS Homo sapiens
52 NDUFS3 4722
Affinity Capture-MS Homo sapiens
53 NAA35 60560
Affinity Capture-MS Homo sapiens
54 PSMA5 5686
Affinity Capture-MS Homo sapiens
55 UQCRC2 7385
Affinity Capture-MS Homo sapiens
56 MYH9 4627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 RBM39 9584
Affinity Capture-MS Homo sapiens
58 TMED3 23423
Affinity Capture-MS Homo sapiens
59 CBR4  
Affinity Capture-MS Homo sapiens
60 HSDL2 84263
Affinity Capture-MS Homo sapiens
61 COX7A2 1347
Affinity Capture-MS Homo sapiens
62 RPL27 6155
Affinity Capture-MS Homo sapiens
63 CFL2 1073
Affinity Capture-MS Homo sapiens
64 ECHS1 1892
Affinity Capture-MS Homo sapiens
65 MEOX2  
Two-hybrid Homo sapiens
66 MED23 9439
Affinity Capture-MS Homo sapiens
67 PSMB5 5693
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
68 ACTB 60
Affinity Capture-MS Homo sapiens
69 CAPZA1 829
Affinity Capture-MS Homo sapiens
70 MYH10 4628
Affinity Capture-MS Homo sapiens
71 PSMB6 5694
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
72 PHF10  
Affinity Capture-MS Homo sapiens
73 OBSL1 23363
Affinity Capture-MS Homo sapiens
74 RPL7 6129
Affinity Capture-MS Homo sapiens
75 CPSF6 11052
Affinity Capture-MS Homo sapiens
76 PSMB1 5689
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
77 UBXN2B  
Affinity Capture-MS Homo sapiens
78 ZDBF2  
Affinity Capture-MS Homo sapiens
79 APRT 353
Affinity Capture-MS Homo sapiens
80 ATP5A1 498
Affinity Capture-MS Homo sapiens
81 SLC25A5 292
Affinity Capture-MS Homo sapiens
82 NOC3L 64318
Affinity Capture-MS Homo sapiens
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