Gene description for HSDL2
Gene name hydroxysteroid dehydrogenase like 2
Gene symbol HSDL2
Other names/aliases C9orf99
SDR13C1
Species Homo sapiens
 Database cross references - HSDL2
ExoCarta ExoCarta_84263
Vesiclepedia VP_84263
Entrez Gene 84263
HGNC 18572
UniProt Q6YN16  
 HSDL2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for HSDL2
Molecular Function
    steroid dehydrogenase activity GO:0016229 IDA
Biological Process
    cholesterol homeostasis GO:0042632 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    peroxisome GO:0005777 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified HSDL2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HSDL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 F2RL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 LHFPL4  
Affinity Capture-MS Homo sapiens
5 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
6 PSMB10 5699
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 PMPCB 9512
Proximity Label-MS Homo sapiens
9 TACO1  
Proximity Label-MS Homo sapiens
10 TSFM 10102
Proximity Label-MS Homo sapiens
11 AUH 549
Proximity Label-MS Homo sapiens
12 VWA8 23078
Proximity Label-MS Homo sapiens
13 KIF3A 11127
Affinity Capture-MS Homo sapiens
14 NEURL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HERC2 8924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MTERF3  
Proximity Label-MS Homo sapiens
17 MTIF2 4528
Proximity Label-MS Homo sapiens
18 CALML3 810
Affinity Capture-MS Homo sapiens
19 NR3C1 2908
Proximity Label-MS Homo sapiens
20 ICT1 3396
Proximity Label-MS Homo sapiens
21 APOO 79135
Proximity Label-MS Homo sapiens
22 LONP1 9361
Proximity Label-MS Homo sapiens
23 EXD2  
Proximity Label-MS Homo sapiens
24 ADRB2  
Affinity Capture-MS Homo sapiens
25 RMND1 55005
Proximity Label-MS Homo sapiens
26 DLD 1738
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 NUP43 348995
Proximity Label-MS Homo sapiens
28 PDHA1 5160
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
29 C6orf203  
Proximity Label-MS Homo sapiens
30 SF1 7536
Co-fractionation Homo sapiens
31 KIF20A 10112
Affinity Capture-MS Homo sapiens
32 FASTKD5  
Proximity Label-MS Homo sapiens
33 FDXR 2232
Co-fractionation Homo sapiens
34 MDH2 4191
Proximity Label-MS Homo sapiens
35 HDAC1 3065
Affinity Capture-MS Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 FBXO6 26270
Affinity Capture-MS Homo sapiens
38 XRCC4  
Affinity Capture-MS Homo sapiens
39 NUP35 129401
Proximity Label-MS Homo sapiens
40 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
41 C21orf33  
Proximity Label-MS Homo sapiens
42 NDUFA8 4702
Co-fractionation Homo sapiens
43 SSBP1 6742
Proximity Label-MS Homo sapiens
44 HSCB 150274
Proximity Label-MS Homo sapiens
45 TRAP1 10131
Co-fractionation Homo sapiens
46 TUFM 7284
Proximity Label-MS Homo sapiens
47 LRPPRC 10128
Proximity Label-MS Homo sapiens
48 MTIF3  
Proximity Label-MS Homo sapiens
49 CAPZB 832
Affinity Capture-MS Homo sapiens
50 RPA3 6119
Proximity Label-MS Homo sapiens
51 MAPK6  
Affinity Capture-MS Homo sapiens
52 C1QBP 708
Proximity Label-MS Homo sapiens
53 GFM1 85476
Proximity Label-MS Homo sapiens
54 RPUSD4 84881
Proximity Label-MS Homo sapiens
55 AARS2  
Proximity Label-MS Homo sapiens
56 TFAM 7019
Proximity Label-MS Homo sapiens
57 FAM133A 286499
Affinity Capture-MS Homo sapiens
58 PMPCA 23203
Proximity Label-MS Homo sapiens
59 COQ9  
Affinity Capture-MS Homo sapiens
60 COX8A  
Proximity Label-MS Homo sapiens
61 DLST 1743
Affinity Capture-MS Homo sapiens
62 MIPEP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 RPUSD3  
Proximity Label-MS Homo sapiens
64 DNPEP 23549
Affinity Capture-MS Homo sapiens
65 TBRG4 9238
Proximity Label-MS Homo sapiens
66 ACAD9 28976
Proximity Label-MS Homo sapiens
67 ZUFSP 221302
Affinity Capture-MS Homo sapiens
68 HINT2 84681
Proximity Label-MS Homo sapiens
69 PEX5 5830
Affinity Capture-MS Homo sapiens
70 TAGLN2 8407
Co-fractionation Homo sapiens
71 UFL1 23376
Affinity Capture-MS Homo sapiens
72 NDUFS6  
Affinity Capture-MS Homo sapiens
73 TRUB2  
Proximity Label-MS Homo sapiens
74 DNAJC9 23234
Affinity Capture-MS Homo sapiens
75 METTL17  
Proximity Label-MS Homo sapiens
76 MTRF1L  
Proximity Label-MS Homo sapiens
77 PRPS1 5631
Affinity Capture-MS Homo sapiens
78 Bub1  
Affinity Capture-MS Mus musculus
79 AGPS 8540
Proximity Label-MS Homo sapiens
80 MRPS26 64949
Proximity Label-MS Homo sapiens
81 TMEM70  
Proximity Label-MS Homo sapiens
82 C9orf72  
Affinity Capture-MS Homo sapiens
83 MCUR1 63933
Proximity Label-MS Homo sapiens
84 PPM1A 5494
Affinity Capture-MS Homo sapiens
85 MTRF1  
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HSDL2 is involved
No pathways found





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