Gene description for ATP5F1
Gene name ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
Gene symbol ATP5F1
Other names/aliases PIG47
Species Homo sapiens
 Database cross references - ATP5F1
ExoCarta ExoCarta_515
Vesiclepedia VP_515
Entrez Gene 515
HGNC 840
MIM 603270
UniProt P24539  
 ATP5F1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for ATP5F1
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IDA
Biological Process
    proton motive force-driven ATP synthesis GO:0015986 IBA
    proton motive force-driven ATP synthesis GO:0015986 NAS
    substantia nigra development GO:0021762 HEP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IDA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    nucleus GO:0005634 HDA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IC
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial matrix GO:0005759 NAS
    membrane GO:0016020 HDA
    proton-transporting ATP synthase complex GO:0045259 IDA
    proton-transporting ATP synthase complex, coupling factor F(o) GO:0045263 IEA
 Experiment description of studies that identified ATP5F1 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for ATP5F1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STARD3NL 83930
Affinity Capture-MS Homo sapiens
2 PDHA1 5160
Proximity Label-MS Homo sapiens
3 ACOT7 11332
Affinity Capture-MS Homo sapiens
4 TAS2R19  
Affinity Capture-MS Homo sapiens
5 AFG3L2 10939
Co-fractionation Homo sapiens
6 SLIRP 81892
Proximity Label-MS Homo sapiens
7 TMCO1 54499
Co-fractionation Homo sapiens
8 ATPIF1 93974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CMAS 55907
Affinity Capture-MS Homo sapiens
10 MTCH1 23787
Co-fractionation Homo sapiens
11 MCM2 4171
Affinity Capture-MS Homo sapiens
12 P2RY1  
Affinity Capture-MS Homo sapiens
13 NDUFS1 4719
Co-fractionation Homo sapiens
14 CHMP1A 5119
Affinity Capture-MS Homo sapiens
15 ATP1B1 481
Co-fractionation Homo sapiens
16 TPM2 7169
Co-fractionation Homo sapiens
17 RPA2 6118
Proximity Label-MS Homo sapiens
18 ARFGAP2 84364
Affinity Capture-MS Homo sapiens
19 TRUB2  
Proximity Label-MS Homo sapiens
20 NDUFB5 4711
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
21 PUS7 54517
Affinity Capture-MS Homo sapiens
22 Atp2a2 11938
Affinity Capture-MS Mus musculus
23 UQCRB 7381
Co-fractionation Homo sapiens
24 HSP90AB1 3326
Co-fractionation Homo sapiens
25 NDUFS7 374291
Co-fractionation Homo sapiens
26 MANF 7873
Affinity Capture-MS Homo sapiens
27 NDUFA4 4697
Affinity Capture-MS Homo sapiens
28 DIEXF  
Affinity Capture-MS Homo sapiens
29 MTRF1  
Proximity Label-MS Homo sapiens
30 GFM2 84340
Proximity Label-MS Homo sapiens
31 CCDC90B  
Proximity Label-MS Homo sapiens
32 ATP5L 10632
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
33 ZMAT2  
Affinity Capture-MS Homo sapiens
34 C7orf55  
Affinity Capture-MS Homo sapiens
35 KIAA1429 25962
Affinity Capture-MS Homo sapiens
36 TSPO 706
Co-fractionation Homo sapiens
37 NDUFB9 4715
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
38 STOM 2040
Co-fractionation Homo sapiens
39 ATP5D 513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
40 Kcnk1  
Affinity Capture-MS Mus musculus
41 VIPR2  
Affinity Capture-MS Homo sapiens
42 NDUFS3 4722
Affinity Capture-MS Homo sapiens
43 CRYZ 1429
Proximity Label-MS Homo sapiens
44 TFAM 7019
Proximity Label-MS Homo sapiens
45 SGPL1 8879
Co-fractionation Homo sapiens
46 PMPCA 23203
Proximity Label-MS Homo sapiens
47 COQ9  
Affinity Capture-MS Homo sapiens
48 HINT2 84681
Proximity Label-MS Homo sapiens
49 PTPMT1 114971
Affinity Capture-MS Homo sapiens
50 CHCHD2  
Affinity Capture-MS Homo sapiens
51 ATP1B2  
Co-fractionation Homo sapiens
52 FBXW7  
Affinity Capture-MS Homo sapiens
53 PRC1 9055
Affinity Capture-MS Homo sapiens
54 C8orf82  
Proximity Label-MS Homo sapiens
55 OPRM1 4988
Affinity Capture-MS Homo sapiens
56 SLC6A5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CHCHD10  
Affinity Capture-MS Homo sapiens
58 SLC25A12 8604
Proximity Label-MS Homo sapiens
59 DDRGK1 65992
Affinity Capture-MS Homo sapiens
60 C10orf35  
Affinity Capture-MS Homo sapiens
61 ABCA2 20
Affinity Capture-MS Homo sapiens
62 MRPS26 64949
Proximity Label-MS Homo sapiens
63 SURF1  
Proximity Label-MS Homo sapiens
64 ATP5O 539
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
65 ATP5C1 509
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FAM136A 84908
Affinity Capture-MS Homo sapiens
67 EXD2  
Proximity Label-MS Homo sapiens
68 FOXQ1  
Proximity Label-MS Homo sapiens
69 HNRNPA1 3178
Co-fractionation Homo sapiens
70 NDUFV1 4723
Co-fractionation Homo sapiens
71 SUGCT  
Affinity Capture-MS Homo sapiens
72 CANX 821
Co-fractionation Homo sapiens
73 ATP5H 10476
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
74 PKN2 5586
Affinity Capture-MS Homo sapiens
75 PMPCB 9512
Proximity Label-MS Homo sapiens
76 TACO1  
Proximity Label-MS Homo sapiens
77 DVL2 1856
Affinity Capture-MS Homo sapiens
78 PLCB3 5331
Affinity Capture-MS Homo sapiens
79 ADCK4 79934
Affinity Capture-MS Homo sapiens
80 RCOR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 HSPD1 3329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SLC25A3 5250
Co-fractionation Homo sapiens
83 RPS2 6187
Co-fractionation Homo sapiens
84 LMAN1 3998
Co-fractionation Homo sapiens
85 MDH2 4191
Proximity Label-MS Homo sapiens
86 GPR35  
Affinity Capture-MS Homo sapiens
87 KLF16  
Affinity Capture-MS Homo sapiens
88 HEXIM1 10614
Affinity Capture-MS Homo sapiens
89 F2RL1  
Affinity Capture-MS Homo sapiens
90 NDUFA9 4704
Co-fractionation Homo sapiens
91 ATP5A1 498
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 C6orf203  
Proximity Label-MS Homo sapiens
93 NDUFS4 4724
Co-fractionation Homo sapiens
94 COX15 1355
Proximity Label-MS Homo sapiens
95 GCGR  
Affinity Capture-MS Homo sapiens
96 FASTKD5  
Proximity Label-MS Homo sapiens
97 NDUFB7 4713
Co-fractionation Homo sapiens
98 PARK2  
Affinity Capture-MS Homo sapiens
99 ATP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 STOML2 30968
Co-fractionation Homo sapiens
101 VRK1 7443
Affinity Capture-MS Homo sapiens
102 BTF3L4 91408
Affinity Capture-MS Homo sapiens
103 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
104 TUFM 7284
Proximity Label-MS Homo sapiens
105 ZMYM3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 CERS2 29956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 KDSR 2531
Affinity Capture-MS Homo sapiens
108 Atp5a1 11946
Affinity Capture-MS Mus musculus
109 PILRB 29990
Affinity Capture-MS Homo sapiens
110 KDM1A 23028
Affinity Capture-MS Homo sapiens
111 FYN 2534
Affinity Capture-MS Homo sapiens
112 LDB3  
Co-fractionation Homo sapiens
113 RELL1 768211
Affinity Capture-MS Homo sapiens
114 SCAMP2 10066
Affinity Capture-MS Homo sapiens
115 FAM134A 79137
Affinity Capture-MS Homo sapiens
116 TRIP10 9322
Affinity Capture-MS Homo sapiens
117 TBRG4 9238
Proximity Label-MS Homo sapiens
118 HUWE1 10075
Affinity Capture-MS Homo sapiens
119 TTC9C 283237
Affinity Capture-MS Homo sapiens
120 BCL6  
Affinity Capture-MS Homo sapiens
121 MCU 90550
Proximity Label-MS Homo sapiens
122 B4GALT3 8703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 MRPL11 65003
Proximity Label-MS Homo sapiens
124 MTRF1L  
Proximity Label-MS Homo sapiens
125 COA3 28958
Co-fractionation Homo sapiens
126 SND1 27044
Co-fractionation Homo sapiens
127 TMEM70  
Proximity Label-MS Homo sapiens
128 NIPSNAP1 8508
Co-fractionation Homo sapiens
129 ATP5B 506
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 YIF1B 90522
Affinity Capture-MS Homo sapiens
131 ATP6V1H 51606
Co-fractionation Homo sapiens
132 MTG2  
Proximity Label-MS Homo sapiens
133 CISD3 284106
Affinity Capture-MS Homo sapiens
134 C16orf72 29035
Affinity Capture-MS Homo sapiens
135 DKK3 27122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 CMSS1  
Affinity Capture-MS Homo sapiens
137 SEC63 11231
Affinity Capture-MS Homo sapiens
138 C15orf48  
Affinity Capture-MS Homo sapiens
139 EPB41L1 2036
Affinity Capture-MS Homo sapiens
140 OTC 5009
Proximity Label-MS Homo sapiens
141 MRPL40 64976
Co-fractionation Homo sapiens
142 TOMM34 10953
Affinity Capture-MS Homo sapiens
143 FAM134C 162427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 MRRF  
Proximity Label-MS Homo sapiens
145 VAPB 9217
Co-fractionation Homo sapiens
146 MRPS12  
Proximity Label-MS Homo sapiens
147 MTIF2 4528
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 ATP5I 521
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
149 UQCRQ 27089
Co-fractionation Homo sapiens
150 HSPA14 51182
Affinity Capture-MS Homo sapiens
151 SDHA 6389
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
152 CLPP 8192
Proximity Label-MS Homo sapiens
153 MTIF3  
Proximity Label-MS Homo sapiens
154 ZNF622 90441
Affinity Capture-MS Homo sapiens
155 PSD2  
Affinity Capture-MS Homo sapiens
156 CCDC109B 55013
Proximity Label-MS Homo sapiens
157 RPUSD3  
Proximity Label-MS Homo sapiens
158 EGFR 1956
Affinity Capture-MS Homo sapiens
159 SH3PXD2B 285590
Affinity Capture-MS Homo sapiens
160 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 ATP1B3 483
Co-fractionation Homo sapiens
162 OPRL1 4987
Affinity Capture-MS Homo sapiens
163 WIBG 84305
Affinity Capture-MS Homo sapiens
164 LPAR1 1902
Affinity Capture-MS Homo sapiens
165 TMEM169  
Affinity Capture-MS Homo sapiens
166 OCIAD1 54940
Co-fractionation Homo sapiens
167 C17orf80 55028
Proximity Label-MS Homo sapiens
168 BLK 640
Affinity Capture-MS Homo sapiens
169 CD27  
Affinity Capture-MS Homo sapiens
170 HADHA 3030
Co-fractionation Homo sapiens
171 CDK2 1017
Affinity Capture-MS Homo sapiens
172 TPM1 7168
Co-fractionation Homo sapiens
173 MTG1  
Proximity Label-MS Homo sapiens
174 RPUSD4 84881
Proximity Label-MS Homo sapiens
175 RPA3 6119
Proximity Label-MS Homo sapiens
176 ATP6V0D1 9114
Co-fractionation Homo sapiens
177 EMC2 9694
Affinity Capture-MS Homo sapiens
178 EI24  
Affinity Capture-MS Homo sapiens
179 DLST 1743
Co-fractionation Homo sapiens
180 TSFM 10102
Proximity Label-MS Homo sapiens
181 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
182 AUH 549
Proximity Label-MS Homo sapiens
183 ACAD9 28976
Proximity Label-MS Homo sapiens
184 CERS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
185 VWA8 23078
Proximity Label-MS Homo sapiens
186 RNF2  
Affinity Capture-MS Homo sapiens
187 VDAC1 7416
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
188 SGCZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
189 TCEA1 6917
Affinity Capture-MS Homo sapiens
190 COX2 4513
Co-fractionation Homo sapiens
191 NTSR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 UHRF1 29128
Affinity Capture-MS Homo sapiens
193 METTL15  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
194 BRD3 8019
Affinity Capture-MS Homo sapiens
195 TPM4 7171
Co-fractionation Homo sapiens
196 MCUR1 63933
Proximity Label-MS Homo sapiens
197 NDUFS2 4720
Co-fractionation Homo sapiens
198 TOMM20 9804
Co-fractionation Homo sapiens
199 CCDC8  
Affinity Capture-MS Homo sapiens
200 P2RY12 64805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
201 TPM3 7170
Co-fractionation Homo sapiens
202 GBAS 2631
Co-fractionation Homo sapiens
203 AP3S1 1176
Affinity Capture-MS Homo sapiens
204 MTERF3  
Proximity Label-MS Homo sapiens
205 DNAJC28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
206 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
207 MRPL42  
Co-fractionation Homo sapiens
208 SLC19A2 10560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
209 ASB3 51130
Affinity Capture-MS Homo sapiens
210 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
211 STK24 8428
Affinity Capture-MS Homo sapiens
212 CLIC1 1192
Two-hybrid Homo sapiens
213 TXNDC16 57544
Affinity Capture-MS Homo sapiens
214 TACR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
215 PARP1 142
Proximity Label-MS Homo sapiens
216 BKRF1  
Affinity Capture-MS
217 TSPAN31 6302
Affinity Capture-MS Homo sapiens
218 ATP5J 522
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
219 PDPR 55066
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
220 TOMM22 56993
Co-fractionation Homo sapiens
221 CCDC43 124808
Affinity Capture-MS Homo sapiens
222 ZFYVE27 118813
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
223 ICT1 3396
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
224 SPP1 6696
Two-hybrid Homo sapiens
225 DSTYK 25778
Affinity Capture-MS Homo sapiens
226 LRPPRC 10128
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
227 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
228 COX5A 9377
Co-fractionation Homo sapiens
229 C17orf89  
Affinity Capture-MS Homo sapiens
230 FKBP9 11328
Co-fractionation Homo sapiens
231 UNC93B1 81622
Affinity Capture-MS Homo sapiens
232 WWOX 51741
Affinity Capture-MS Homo sapiens
233 BAZ2A  
Affinity Capture-MS Homo sapiens
234 STK26 51765
Affinity Capture-MS Homo sapiens
235 HSP90AA1 3320
Co-fractionation Homo sapiens
236 SHMT2 6472
Co-fractionation Homo sapiens
237 C21orf33  
Proximity Label-MS Homo sapiens
238 SSBP1 6742
Proximity Label-MS Homo sapiens
239 AASS  
Co-fractionation Homo sapiens
240 SFXN1 94081
Co-fractionation Homo sapiens
241 VDAC2 7417
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
242 PTPN5  
Affinity Capture-MS Homo sapiens
243 Samm50  
Affinity Capture-MS Mus musculus
244 ATP6V0C 527
Affinity Capture-MS Homo sapiens
245 LONP1 9361
Proximity Label-MS Homo sapiens
246 MGST1 4257
Co-fractionation Homo sapiens
247 GFM1 85476
Proximity Label-MS Homo sapiens
248 TEFM  
Proximity Label-MS Homo sapiens
249 SLC27A3 11000
Affinity Capture-MS Homo sapiens
250 C19orf52  
Proximity Label-MS Homo sapiens
251 SLK 9748
Affinity Capture-MS Homo sapiens
252 CYB5R3 1727
Co-fractionation Homo sapiens
253 ACO2 50
Co-fractionation Homo sapiens
254 GRSF1 2926
Proximity Label-MS Homo sapiens
255 C1QBP 708
Proximity Label-MS Homo sapiens
256 SERBP1 26135
Affinity Capture-MS Homo sapiens
257 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
258 Tnpo1 238799
Affinity Capture-MS Mus musculus
259 CUL7 9820
Affinity Capture-MS Homo sapiens
260 VIPR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
261 COX7C 1350
Co-fractionation Homo sapiens
262 SH2D4A 63898
Affinity Capture-MS Homo sapiens
263 HTR1B  
Affinity Capture-MS Homo sapiens
264 CCR6  
Affinity Capture-MS Homo sapiens
265 METTL17  
Proximity Label-MS Homo sapiens
266 ATXN3 4287
Affinity Capture-MS Homo sapiens
267 GRIA2  
Affinity Capture-MS Homo sapiens
268 FPR2  
Affinity Capture-MS Homo sapiens
269 DDX28  
Proximity Label-MS Homo sapiens
270 FAM50A 9130
Affinity Capture-MS Homo sapiens
271 VDAC3 7419
Co-fractionation Homo sapiens
272 MTCH2 23788
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
273 YME1L1 10730
Co-fractionation Homo sapiens
274 SP3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here