Gene description for TOMM20
Gene name translocase of outer mitochondrial membrane 20 homolog (yeast)
Gene symbol TOMM20
Other names/aliases MAS20
MOM19
TOM20
Species Homo sapiens
 Database cross references - TOMM20
ExoCarta ExoCarta_9804
Vesiclepedia VP_9804
Entrez Gene 9804
HGNC 20947
MIM 601848
UniProt Q15388  
 TOMM20 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
 Gene ontology annotations for TOMM20
Molecular Function
    protein binding GO:0005515 IPI
    protein transmembrane transporter activity GO:0008320 IBA
    protein-transporting ATPase activity GO:0015450 IDA
    mitochondrion targeting sequence binding GO:0030943 IBA
    mitochondrion targeting sequence binding GO:0030943 IDA
    unfolded protein binding GO:0051082 IDA
Biological Process
    protein targeting to mitochondrion GO:0006626 IDA
    response to muscle activity GO:0014850 IEA
    tRNA import into mitochondrion GO:0016031 IBA
    protein import into mitochondrial matrix GO:0030150 IBA
    protein insertion into mitochondrial outer membrane GO:0045040 NAS
    response to 3,3',5-triiodo-L-thyronine GO:1905242 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 NAS
    mitochondrial outer membrane GO:0005741 TAS
    mitochondrial outer membrane translocase complex GO:0005742 IBA
    mitochondrial outer membrane translocase complex GO:0005742 NAS
    mitochondrial outer membrane translocase complex GO:0005742 TAS
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 IDA
    cell periphery GO:0071944 IEA
    sperm midpiece GO:0097225 ISS
    migrasome GO:0140494 IEA
    TOM complex GO:0140596 NAS
 Experiment description of studies that identified TOMM20 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for TOMM20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UTP18  
Affinity Capture-MS Homo sapiens
4 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
5 BPHL 670
Affinity Capture-MS Homo sapiens
6 DNAJC11 55735
Affinity Capture-MS Homo sapiens
7 MAFA  
Cross-Linking-MS (XL-MS) Homo sapiens
8 RAB1A 5861
Affinity Capture-MS Homo sapiens
9 Psmb5 19173
Affinity Capture-MS Mus musculus
10 UTP11L  
Cross-Linking-MS (XL-MS) Homo sapiens
11 TPP1 1200
Affinity Capture-MS Homo sapiens
12 BTF3 689
Affinity Capture-MS Homo sapiens
13 BIRC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 UBC 7316
Co-localization Homo sapiens
15 TOMM22 56993
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
16 MAP1LC3B 81631
Co-localization Homo sapiens
17 TIMM17B  
Co-fractionation Homo sapiens
18 PRC1 9055
Affinity Capture-MS Homo sapiens
19 TOMM7 54543
Co-fractionation Homo sapiens
20 HIST2H3PS2 440686
Affinity Capture-MS Homo sapiens
21 ABCE1 6059
Affinity Capture-MS Homo sapiens
22 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
23 TMEM192 201931
Affinity Capture-MS Homo sapiens
24 ATG16L1 55054
Affinity Capture-MS Homo sapiens
25 TOMM40 10452
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
26 ABCC2 1244
Co-fractionation Homo sapiens
27 MYCN  
Affinity Capture-MS Homo sapiens
28 FAF2 23197
Affinity Capture-MS Homo sapiens
29 NCSTN 23385
Affinity Capture-MS Homo sapiens
30 SLC25A3 5250
Co-fractionation Homo sapiens
31 RAB1B 81876
Affinity Capture-MS Homo sapiens
32 NDUFS4 4724
Co-fractionation Homo sapiens
33 SFXN3 81855
Co-fractionation Homo sapiens
34 BAG6 7917
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
35 FAM107A  
Affinity Capture-MS Homo sapiens
36 SUPT16H 11198
Affinity Capture-MS Homo sapiens
37 RPA2 6118
Proximity Label-MS Homo sapiens
38 RAB2A 5862
Affinity Capture-MS Homo sapiens
39 RPA4  
Proximity Label-MS Homo sapiens
40 PML 5371
Affinity Capture-MS Homo sapiens
41 CUL2 8453
Affinity Capture-MS Homo sapiens
42 H2AFY 9555
Affinity Capture-MS Homo sapiens
43 HAP1  
Two-hybrid Homo sapiens
44 Nup155  
Affinity Capture-MS Mus musculus
45 SLC25A5 292
Co-fractionation Homo sapiens
46 ATXN1 6310
Two-hybrid Homo sapiens
47 PTN  
Two-hybrid Homo sapiens
48 LIMA1 51474
Affinity Capture-MS Homo sapiens
49 SEC63 11231
Affinity Capture-MS Homo sapiens
50 OTC 5009
Reconstituted Complex Homo sapiens
51 OTUD1 220213
Affinity Capture-Western Homo sapiens
52 SARM1 23098
Affinity Capture-Western Homo sapiens
53 SRSF1 6426
Co-fractionation Homo sapiens
54 SNCA 6622
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
55 TRAF6 7189
Affinity Capture-Western Homo sapiens
56 ATP5F1 515
Co-fractionation Homo sapiens
57 EGFR 1956
Negative Genetic Homo sapiens
58 FKBP8 23770
Affinity Capture-MS Homo sapiens
59 KIAA1377  
Two-hybrid Homo sapiens
60 H3F3B 3021
Affinity Capture-MS Homo sapiens
61 OCIAD1 54940
Affinity Capture-MS Homo sapiens
62 NXF1 10482
Affinity Capture-RNA Homo sapiens
63 TOMM70A 9868
Co-fractionation Homo sapiens
64 PPHLN1  
Two-hybrid Homo sapiens
65 MAP7D1 55700
Cross-Linking-MS (XL-MS) Homo sapiens
66 MAPK6  
Affinity Capture-MS Homo sapiens
67 ACTR5 79913
Affinity Capture-MS Homo sapiens
68 MYC  
Affinity Capture-MS Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 DDRGK1 65992
Affinity Capture-MS Homo sapiens
71 VDAC1 7416
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
72 SLC25A6 293
Co-fractionation Homo sapiens
73 MSN 4478
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
74 MAP7D2 256714
Cross-Linking-MS (XL-MS) Homo sapiens
75 AKAP1 8165
Proximity Label-MS Homo sapiens
76 FEZ1  
Two-hybrid Homo sapiens
77 BKRF1  
Affinity Capture-MS
78 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
79 FEM1B 10116
Affinity Capture-MS Homo sapiens
80 DDX58 23586
Affinity Capture-RNA Homo sapiens
81 SFXN1 94081
Co-fractionation Homo sapiens
82 UFL1 23376
Affinity Capture-MS Homo sapiens
83 USP30  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
84 MOV10 4343
Affinity Capture-RNA Homo sapiens
85 BCL2  
Reconstituted Complex Homo sapiens
86 UCP1  
Reconstituted Complex Homo sapiens
87 NEDD1 121441
Affinity Capture-MS Homo sapiens
88 RAB7A 7879
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
89 PINK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
90 CYB5R3 1727
Affinity Capture-MS Homo sapiens
91 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
92 KRAS 3845
Negative Genetic Homo sapiens
93 FBXO7 25793
Reconstituted Complex Homo sapiens
94 POLG  
Affinity Capture-Western Homo sapiens
95 APOA1 335
Two-hybrid Homo sapiens
96 NDUFB4 4710
Co-fractionation Homo sapiens
View the network image/svg+xml



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