Experiment description of studies that identified FAM136A in sEVs

1 Experiment ID 426 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 427 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 434 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 435 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for FAM136A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 COLGALT2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 ATP5H 10476 Affinity Capture-MS Homo sapiens 3 ECH1 1891 Affinity Capture-MS Homo sapiens 4 RGPD5   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 PHB2 11331 Proximity Label-MS Homo sapiens 6 TEX37   Affinity Capture-MS Homo sapiens 7 N4BP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 AK4 205 Co-fractionation Homo sapiens 9 AIFM1 9131 Proximity Label-MS Homo sapiens 10 CYBRD1 79901 Affinity Capture-MS Homo sapiens 11 SLC25A53   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 LARP4B 23185 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 ATP5F1 515 Affinity Capture-MS Homo sapiens 14 STRIP2   Affinity Capture-MS Homo sapiens 15 RGPD8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 PCMT1 5110 Co-fractionation Homo sapiens 17 LDHB 3945 Cross-Linking-MS (XL-MS) Homo sapiens 18 ASPSCR1 79058 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 ZNF703   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 COX10   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 NKX3-1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 UPK3B   Affinity Capture-MS Homo sapiens 23 NUDCD3 23386 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 24 IDE 3416 Affinity Capture-MS Homo sapiens 25 PRDX5 25824 Co-fractionation Homo sapiens 26 KRTAP15-1   Affinity Capture-MS Homo sapiens 27 ACOX1 51 Affinity Capture-MS Homo sapiens 28 RBMS1 5937 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 XRCC6BP1   Affinity Capture-MS Homo sapiens 30 PCDHA3   Affinity Capture-MS Homo sapiens 31 RNASEH2A 10535 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 OBFC1 79991 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 OTUB1 55611 Affinity Capture-MS Homo sapiens 34 YTHDF1 54915 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 NUP35 129401 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 NUP98 4928 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 MRPS16   Affinity Capture-MS Homo sapiens 38 SFXN1 94081 Proximity Label-MS Homo sapiens 39 HCCS 3052 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 DAZ2   Affinity Capture-MS Homo sapiens 41 RBFOX2 23543 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 CDYL 9425 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 KRTAP19-7   Affinity Capture-MS Homo sapiens 44 MYDGF 56005 Cross-Linking-MS (XL-MS) Homo sapiens 45 AMPD2 271 Affinity Capture-MS Homo sapiens 46 RNF138   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 47 ANGEL2 90806 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 GSE1 23199 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 LMNB1 4001 Affinity Capture-MS Homo sapiens 50 DHX30 22907 Affinity Capture-MS Homo sapiens 51 PIGT 51604 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 PLS1 5357 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 KRTAP19-4   Affinity Capture-MS Homo sapiens 54 TTC4   Cross-Linking-MS (XL-MS) Homo sapiens 55 CHCHD2   Affinity Capture-MS Homo sapiens 56 DNAJB9 4189 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 POLDIP2 26073 Affinity Capture-MS Homo sapiens 58 DUSP14 11072 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 C1QBP 708 Affinity Capture-MS Homo sapiens 60 SERBP1 26135 Affinity Capture-MS Homo sapiens 61 SRMS   Affinity Capture-MS Homo sapiens 62 ENO1 2023 Co-fractionation Homo sapiens 63 GFER   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 64 CYB5R3 1727 Affinity Capture-MS Homo sapiens 65 KRTAP6-1   Affinity Capture-MS Homo sapiens 66 SRC 6714 Affinity Capture-MS Homo sapiens 67 KRTAP13-3   Affinity Capture-MS Homo sapiens 68 HNRNPUL2 221092 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 69 RBFOX3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 PIK3C2A 5286 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 71 BCKDHA 593 Affinity Capture-MS Homo sapiens 72 PAQR4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 73 TIMP3 7078 Affinity Capture-MS Homo sapiens 74 NDUFS6   Affinity Capture-MS Homo sapiens 75 CEBPA   Protein-peptide Homo sapiens 76 FAM136A 84908 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 77 ACSL5 51703 Affinity Capture-MS Homo sapiens 78 ECSIT 51295 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 79 RPS6KA1 6195 Affinity Capture-MS Homo sapiens 80 CLPB 81570 Affinity Capture-MS Homo sapiens View the network

Pathways in which FAM136A is involved

No pathways found