Gene description for Atp2a2
Gene name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Gene symbol Atp2a2
Other names/aliases 9530097L16Rik
D5Wsu150e
SERCA2
SERCA2B
mKIAA4195
Species Mus musculus
 Database cross references - Atp2a2
ExoCarta ExoCarta_11938
Vesiclepedia VP_11938
Entrez Gene 11938
UniProt O55143  
 Atp2a2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp2a2
Molecular Function
    P-type calcium transporter activity GO:0005388 IDA
    P-type calcium transporter activity GO:0005388 IMP
    P-type calcium transporter activity GO:0005388 ISO
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IEA
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    lutropin-choriogonadotropic hormone receptor binding GO:0031775 ISO
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    S100 protein binding GO:0044548 IEA
    S100 protein binding GO:0044548 ISO
    P-type calcium transporter activity involved in regulation of cardiac muscle cell membrane potential GO:0086039 IBA
    P-type calcium transporter activity involved in regulation of cardiac muscle cell membrane potential GO:0086039 ISO
    P-type calcium transporter activity involved in regulation of cardiac muscle cell membrane potential GO:0086039 ISS
    lncRNA binding GO:0106222 IPI
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISS
    regulation of the force of heart contraction GO:0002026 IGI
    calcium ion transport GO:0006816 ISO
    intracellular calcium ion homeostasis GO:0006874 IBA
    intracellular calcium ion homeostasis GO:0006874 IDA
    intracellular calcium ion homeostasis GO:0006874 IGI
    intracellular calcium ion homeostasis GO:0006874 IMP
    intracellular calcium ion homeostasis GO:0006874 ISO
    regulation of muscle contraction GO:0006937 TAS
    ER-nucleus signaling pathway GO:0006984 IMP
    organelle organization GO:0006996 IMP
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 IDA
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 IBA
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 ISO
    transition between fast and slow fiber GO:0014883 IDA
    cardiac muscle hypertrophy in response to stress GO:0014898 IDA
    autophagosome membrane docking GO:0016240 ISO
    autophagosome membrane docking GO:0016240 ISS
    endoplasmic reticulum calcium ion homeostasis GO:0032469 ISO
    positive regulation of endoplasmic reticulum calcium ion concentration GO:0032470 IMP
    positive regulation of endoplasmic reticulum calcium ion concentration GO:0032470 ISO
    T-tubule organization GO:0033292 IMP
    cellular response to oxidative stress GO:0034599 IGI
    response to peptide hormone GO:0043434 ISO
    negative regulation of heart contraction GO:0045822 IGI
    relaxation of cardiac muscle GO:0055119 ISO
    neuron cellular homeostasis GO:0070050 IGI
    neuron cellular homeostasis GO:0070050 IMP
    sarcoplasmic reticulum calcium ion transport GO:0070296 IMP
    sarcoplasmic reticulum calcium ion transport GO:0070296 ISO
    calcium ion transmembrane transport GO:0070588 IBA
    calcium ion transmembrane transport GO:0070588 IMP
    calcium ion transmembrane transport GO:0070588 ISO
    regulation of cardiac muscle cell membrane potential GO:0086036 IEA
    regulation of cardiac muscle cell membrane potential GO:0086036 ISO
    regulation of cardiac muscle cell action potential involved in regulation of contraction GO:0098909 ISO
    organelle localization by membrane tethering GO:0140056 ISO
    organelle localization by membrane tethering GO:0140056 ISS
    negative regulation of receptor binding GO:1900121 ISO
    regulation of calcium ion-dependent exocytosis of neurotransmitter GO:1903233 IMP
    calcium ion transport from cytosol to endoplasmic reticulum GO:1903515 IEA
    calcium ion transport from cytosol to endoplasmic reticulum GO:1903515 ISO
    calcium ion import into sarcoplasmic reticulum GO:1990036 IMP
    calcium ion import into sarcoplasmic reticulum GO:1990036 ISO
    mitochondrion-endoplasmic reticulum membrane tethering GO:1990456 ISO
    mitochondrion-endoplasmic reticulum membrane tethering GO:1990456 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    intercalated disc GO:0014704 ISO
    longitudinal sarcoplasmic reticulum GO:0014801 ISO
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    sarcoplasmic reticulum GO:0016529 IDA
    sarcoplasmic reticulum GO:0016529 ISO
    sarcoplasmic reticulum GO:0016529 TAS
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 ISO
    protein-containing complex GO:0032991 ISO
    sarcoplasmic reticulum membrane GO:0033017 IEA
    sarcoplasmic reticulum membrane GO:0033017 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    apical ectoplasmic specialization GO:0061831 ISO
    calcium ion-transporting ATPase complex GO:0090534 ISO
    ribbon synapse GO:0097470 IDA
    plasma membrane bounded cell projection GO:0120025 ISO
 Experiment description of studies that identified Atp2a2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp2a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atp2b1 67972
Affinity Capture-Western Mus musculus
2 PHB 5245
Affinity Capture-MS Homo sapiens
3 Nphs2  
Affinity Capture-MS Mus musculus
4 SLC35E1 79939
Affinity Capture-MS Homo sapiens
5 TMCO1 54499
Affinity Capture-MS Homo sapiens
6 CLCN7 1186
Affinity Capture-MS Homo sapiens
7 ESYT2 57488
Affinity Capture-MS Homo sapiens
8 SLC1A5 6510
Affinity Capture-MS Homo sapiens
9 STT3A 3703
Affinity Capture-MS Homo sapiens
10 VTI1B 10490
Affinity Capture-MS Homo sapiens
11 TMEM161A  
Affinity Capture-MS Homo sapiens
12 DHCR24 1718
Affinity Capture-MS Homo sapiens
13 DHCR7 1717
Affinity Capture-MS Homo sapiens
14 Lnx2  
Affinity Capture-MS Mus musculus
15 TMEM120A 83862
Affinity Capture-MS Homo sapiens
16 HTT 3064
Affinity Capture-MS Homo sapiens
17 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
18 Kcna3  
Affinity Capture-MS Mus musculus
19 Kctd13  
Affinity Capture-MS Mus musculus
20 SEC22B 9554
Affinity Capture-MS Homo sapiens
21 Atp2a2 11938
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
22 ATP5F1 515
Affinity Capture-MS Homo sapiens
23 Nos2  
Co-fractionation Mus musculus
24 INTS2  
Affinity Capture-MS Homo sapiens
25 PTDSS1 9791
Affinity Capture-MS Homo sapiens
26 Iqcb1  
Affinity Capture-MS Mus musculus
27 YIPF5 81555
Affinity Capture-MS Homo sapiens
28 OCIAD1 54940
Affinity Capture-MS Homo sapiens
29 Mettl21e  
Affinity Capture-MS Mus musculus
30 Tmem173  
Proximity Label-MS Mus musculus
31 RABL3 285282
Affinity Capture-MS Homo sapiens
32 PHB2 11331
Affinity Capture-MS Homo sapiens
33 POLQ  
Affinity Capture-MS Homo sapiens
34 ALG8  
Affinity Capture-MS Homo sapiens
35 SLC35F6 54978
Affinity Capture-MS Homo sapiens
36 SLC43A3 29015
Affinity Capture-MS Homo sapiens
37 Unk  
Affinity Capture-RNA Mus musculus
38 Twist1  
Proximity Label-MS Mus musculus
39 PON2 5445
Affinity Capture-MS Homo sapiens
40 TAP2 6891
Affinity Capture-MS Homo sapiens
41 Sstr3  
Proximity Label-MS Mus musculus
42 ABCD3 5825
Affinity Capture-MS Homo sapiens
43 Eed  
Affinity Capture-MS Mus musculus
44 Mapt  
Affinity Capture-MS Mus musculus
45 TMEM33 55161
Affinity Capture-MS Homo sapiens
46 Tia1  
Affinity Capture-MS Mus musculus
47 ATP5O 539
Affinity Capture-MS Homo sapiens
48 ATP5B 506
Affinity Capture-MS Homo sapiens
49 Fancd2  
Affinity Capture-MS Mus musculus
50 ATP5A1 498
Affinity Capture-MS Homo sapiens
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