Gene description for BLK
Gene name BLK proto-oncogene, Src family tyrosine kinase
Gene symbol BLK
Other names/aliases MODY11
Species Homo sapiens
 Database cross references - BLK
ExoCarta ExoCarta_640
Vesiclepedia VP_640
Entrez Gene 640
HGNC 1057
MIM 191305
UniProt P51451  
 BLK identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for BLK
Molecular Function
    protein tyrosine kinase activity GO:0004713 IDA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IMP
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    cell differentiation GO:0030154 IBA
    positive regulation of insulin secretion GO:0032024 IMP
    positive regulation of protein binding GO:0032092 IMP
    intracellular signal transduction GO:0035556 TAS
    B cell receptor signaling pathway GO:0050853 IDA
    B cell receptor signaling pathway GO:0050853 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
 Experiment description of studies that identified BLK in sEVs
1
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BLK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDELR3 11015
Affinity Capture-MS Homo sapiens
2 ISG15 9636
Affinity Capture-MS Homo sapiens
3 ATPIF1 93974
Affinity Capture-MS Homo sapiens
4 MBTD1  
Affinity Capture-MS Homo sapiens
5 YTHDF1 54915
Affinity Capture-MS Homo sapiens
6 CDC45  
Affinity Capture-MS Homo sapiens
7 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
8 NFATC1 4772
Affinity Capture-MS Homo sapiens
9 RPL37A 6168
Affinity Capture-MS Homo sapiens
10 ANKRD9  
Affinity Capture-MS Homo sapiens
11 UBE3A 7337
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 SOCS1  
Biochemical Activity Homo sapiens
13 DNAJC25 548645
Affinity Capture-MS Homo sapiens
14 GRB10 2887
Two-hybrid Homo sapiens
15 CD79A  
Affinity Capture-Western Homo sapiens
16 GNGT1  
Affinity Capture-MS Homo sapiens
17 STAP2 55620
Two-hybrid Homo sapiens
18 SLC30A4 7782
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MTRF1  
Affinity Capture-MS Homo sapiens
20 ATP5L 10632
Affinity Capture-MS Homo sapiens
21 SYCP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ZBTB8A  
Two-hybrid Homo sapiens
23 GNG12 55970
Affinity Capture-MS Homo sapiens
24 ATP5D 513
Affinity Capture-MS Homo sapiens
25 PLCG2 5336
Reconstituted Complex Homo sapiens
26 JAKMIP1 152789
Two-hybrid Homo sapiens
27 ZMAT1  
Two-hybrid Homo sapiens
28 Nedd4 25489
Biochemical Activity Rattus norvegicus
29 TLR3  
Affinity Capture-MS Homo sapiens
30 APOC3 345
Affinity Capture-MS Homo sapiens
31 SENP1  
Affinity Capture-MS Homo sapiens
32 FBXO17 115290
Affinity Capture-MS Homo sapiens
33 STAT3 6774
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 TDP2 51567
Two-hybrid Homo sapiens
35 CDH16  
Affinity Capture-MS Homo sapiens
36 CHIT1  
Affinity Capture-MS Homo sapiens
37 HSP90B1 7184
Affinity Capture-MS Homo sapiens
38 ATP5O 539
Affinity Capture-MS Homo sapiens
39 USMG5 84833
Affinity Capture-MS Homo sapiens
40 TULP3 7289
Affinity Capture-MS Homo sapiens
41 Mbp 17196
Protein-peptide Mus musculus
42 ATP5H 10476
Affinity Capture-MS Homo sapiens
43 SSBP3  
Two-hybrid Homo sapiens
44 SOCS3 9021
Two-hybrid Homo sapiens
45 ERBB2 2064
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
46 NCSTN 23385
Affinity Capture-MS Homo sapiens
47 CINP  
Affinity Capture-MS Homo sapiens
48 Bcas2  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
49 CCL15  
Affinity Capture-MS Homo sapiens
50 CDKN2AIP  
Affinity Capture-MS Homo sapiens
51 KIT 3815
Reconstituted Complex Homo sapiens
52 RNF4 6047
Affinity Capture-MS Homo sapiens
53 TGM2 7052
Affinity Capture-MS Homo sapiens
54 C3orf62  
Affinity Capture-MS Homo sapiens
55 ERBB3 2065
Reconstituted Complex Homo sapiens
56 OR5C1  
Affinity Capture-MS Homo sapiens
57 CCDC33 80125
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 CBL 867
Affinity Capture-Western Homo sapiens
59 SCGB1A1  
Affinity Capture-MS Homo sapiens
60 EEF1E1 9521
Affinity Capture-MS Homo sapiens
61 ARL5B  
Affinity Capture-MS Homo sapiens
62 MYH9 4627
Affinity Capture-MS Homo sapiens
63 FBXL22  
Affinity Capture-MS Homo sapiens
64 SNRPD1 6632
Affinity Capture-MS Homo sapiens
65 BCL2L1 598
Reconstituted Complex Homo sapiens
66 PIK3R1 5295
Two-hybrid Homo sapiens
67 LRTM2  
Affinity Capture-MS Homo sapiens
68 CDX1  
Affinity Capture-MS Homo sapiens
69 LRRTM4  
Affinity Capture-MS Homo sapiens
70 ATP5B 506
Affinity Capture-MS Homo sapiens
71 CAMK1 8536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 MET 4233
Reconstituted Complex Homo sapiens
73 SPATA22  
Affinity Capture-MS Homo sapiens
74 BIRC2  
Two-hybrid Homo sapiens
75 CXCR4 7852
Affinity Capture-MS Homo sapiens
76 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
77 SLA  
Two-hybrid Homo sapiens
78 ATP5I 521
Affinity Capture-MS Homo sapiens
79 CD79B 974
Affinity Capture-Western Homo sapiens
80 PIGP  
Affinity Capture-MS Homo sapiens
81 IKZF3  
Two-hybrid Homo sapiens
82 SH2D1B  
Two-hybrid Homo sapiens
83 EEF2 1938
Affinity Capture-MS Homo sapiens
84 PARP12 64761
Affinity Capture-MS Homo sapiens
85 ATP5F1 515
Affinity Capture-MS Homo sapiens
86 ERBB4 2066
Reconstituted Complex Homo sapiens
87 EFS  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
88 EGFR 1956
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Reconstituted Complex Homo sapiens
89 EFCAB11  
Affinity Capture-MS Homo sapiens
90 CNKSR3 154043
Affinity Capture-MS Homo sapiens
91 TRAF3 7187
Affinity Capture-Western Homo sapiens
92 GAB1  
Reconstituted Complex Homo sapiens
93 ATP5J2 9551
Affinity Capture-MS Homo sapiens
94 ZMYM6  
Affinity Capture-MS Homo sapiens
95 FXR2 9513
Two-hybrid Homo sapiens
96 ATP5E 514
Affinity Capture-MS Homo sapiens
97 A4GNT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 TLR2 7097
Affinity Capture-MS Homo sapiens
99 BBX 56987
Affinity Capture-MS Homo sapiens
100 FAM217B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 TSR1 55720
Affinity Capture-MS Homo sapiens
102 AVPR2  
Affinity Capture-MS Homo sapiens
103 AP3S1 1176
Affinity Capture-MS Homo sapiens
104 KCNE1  
Affinity Capture-MS Homo sapiens
105 RNASE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 ATP5J 522
Affinity Capture-MS Homo sapiens
107 PHB2 11331
Affinity Capture-MS Homo sapiens
108 GLRA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 USP25  
Affinity Capture-MS Homo sapiens
110 ATP5A1 498
Affinity Capture-MS Homo sapiens
111 ABCA9 10350
Affinity Capture-MS Homo sapiens
112 C15orf53  
Affinity Capture-MS Homo sapiens
113 FXR1 8087
Two-hybrid Homo sapiens
114 CTAG1B  
Affinity Capture-MS Homo sapiens
115 GAD1  
Affinity Capture-MS Homo sapiens
116 ZNF774  
Two-hybrid Homo sapiens
117 ANKRD54  
Affinity Capture-MS Homo sapiens
118 APBB3  
Affinity Capture-MS Homo sapiens
119 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
120 RTN1 6252
Affinity Capture-MS Homo sapiens
121 EDEM2  
Affinity Capture-MS Homo sapiens
122 SUGP2  
Affinity Capture-MS Homo sapiens
123 GAPDH 2597
Affinity Capture-MS Homo sapiens
124 BCL2  
Reconstituted Complex Homo sapiens
125 YBX3 8531
Affinity Capture-MS Homo sapiens
126 GLYR1 84656
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 ATP5C1 509
Affinity Capture-MS Homo sapiens
128 CTNND1 1500
Affinity Capture-MS Homo sapiens
129 AR 367
Reconstituted Complex Homo sapiens
130 CCDC96  
Affinity Capture-MS Homo sapiens
131 ZBTB42  
Two-hybrid Homo sapiens
132 HSF2BP  
Two-hybrid Homo sapiens
133 ALS2CR12  
Two-hybrid Homo sapiens
134 CYTIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 SLC2A13 114134
Affinity Capture-MS Homo sapiens
136 CXCL16 58191
Affinity Capture-MS Homo sapiens
137 TAGAP  
Affinity Capture-MS Homo sapiens
138 PIK3R3 8503
Two-hybrid Homo sapiens
View the network image/svg+xml



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