Gene description for TLR2
Gene name toll-like receptor 2
Gene symbol TLR2
Other names/aliases CD282
TIL4
Species Homo sapiens
 Database cross references - TLR2
ExoCarta ExoCarta_7097
Vesiclepedia VP_7097
Entrez Gene 7097
HGNC 11848
MIM 603028
UniProt O60603  
 TLR2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast milk 17641064    
 Gene ontology annotations for TLR2
Molecular Function
    lipopolysaccharide binding GO:0001530 IDA
    amyloid-beta binding GO:0001540 IDA
    amyloid-beta binding GO:0001540 ISS
    lipopolysaccharide immune receptor activity GO:0001875 TAS
    transmembrane signaling receptor activity GO:0004888 IEA
    protein binding GO:0005515 IPI
    Toll-like receptor binding GO:0035325 IPI
    signaling receptor activity GO:0038023 IBA
    pattern recognition receptor activity GO:0038187 IDA
    triacyl lipopeptide binding GO:0042497 IBA
    triacyl lipopeptide binding GO:0042497 IDA
    identical protein binding GO:0042802 IPI
    peptidoglycan binding GO:0042834 IDA
    protein-containing complex binding GO:0044877 IPI
    NAD+ nucleotidase, cyclic ADP-ribose generating GO:0061809 IEA
Biological Process
    response to hypoxia GO:0001666 IEA
    microglial cell activation GO:0001774 IEA
    toll-like receptor signaling pathway GO:0002224 IBA
    toll-like receptor signaling pathway GO:0002224 IDA
    toll-like receptor signaling pathway GO:0002224 ISS
    leukotriene metabolic process GO:0006691 IEA
    apoptotic process GO:0006915 TAS
    inflammatory response GO:0006954 IBA
    immune response GO:0006955 TAS
    signal transduction GO:0007165 TAS
    I-kappaB phosphorylation GO:0007252 IDA
    learning GO:0007612 ISS
    negative regulation of cell population proliferation GO:0008285 IEA
    response to toxic substance GO:0009636 IEA
    positive regulation of gene expression GO:0010628 IMP
    microglia development GO:0014005 ISS
    positive regulation of Wnt signaling pathway GO:0030177 IMP
    lipopolysaccharide-mediated signaling pathway GO:0031663 IEA
    central nervous system myelin formation GO:0032289 IEA
    response to progesterone GO:0032570 IEA
    positive regulation of chemokine production GO:0032722 IDA
    positive regulation of interferon-beta production GO:0032728 ISS
    positive regulation of interleukin-10 production GO:0032733 IEA
    positive regulation of interleukin-12 production GO:0032735 ISS
    positive regulation of interleukin-18 production GO:0032741 ISS
    positive regulation of interleukin-6 production GO:0032755 IDA
    positive regulation of interleukin-6 production GO:0032755 IGI
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of interleukin-8 production GO:0032757 IGI
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    response to insulin GO:0032868 IEA
    toll-like receptor 2 signaling pathway GO:0034134 IDA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 IDA
    detection of triacyl bacterial lipopeptide GO:0042495 IDA
    detection of diacyl bacterial lipopeptide GO:0042496 IDA
    innate immune response GO:0045087 NAS
    innate immune response GO:0045087 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    nitric oxide metabolic process GO:0046209 IEA
    positive regulation of oligodendrocyte differentiation GO:0048714 IEA
    positive regulation of inflammatory response GO:0050729 IC
    positive regulation of inflammatory response GO:0050729 IGI
    negative regulation of phagocytosis GO:0050765 ISS
    defense response to Gram-positive bacterium GO:0050830 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    defense response to virus GO:0051607 IEP
    negative regulation of synapse assembly GO:0051964 ISS
    response to fatty acid GO:0070542 IEA
    cellular response to bacterial lipopeptide GO:0071221 TAS
    cellular response to lipoteichoic acid GO:0071223 IDA
    cellular response to lipoteichoic acid GO:0071223 IMP
    cellular response to type II interferon GO:0071346 IDA
    cellular response to diacyl bacterial lipopeptide GO:0071726 IDA
    cellular response to diacyl bacterial lipopeptide GO:0071726 IDA
    cellular response to triacyl bacterial lipopeptide GO:0071727 IDA
    cellular response to triacyl bacterial lipopeptide GO:0071727 IDA
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IDA
    positive regulation of cellular response to macrophage colony-stimulating factor stimulus GO:1903974 IDA
    positive regulation of matrix metallopeptidase secretion GO:1904466 IGI
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    secretory granule membrane GO:0030667 TAS
    phagocytic vesicle membrane GO:0030670 IEA
    Toll-like receptor 1-Toll-like receptor 2 protein complex GO:0035354 IDA
    Toll-like receptor 1-Toll-like receptor 2 protein complex GO:0035354 IPI
    Toll-like receptor 2-Toll-like receptor 6 protein complex GO:0035355 IDA
    Toll-like receptor 2-Toll-like receptor 6 protein complex GO:0035355 IPI
    cell projection GO:0042995 IEA
    receptor complex GO:0043235 IBA
    receptor complex GO:0043235 IPI
    cell body GO:0044297 IEA
    membrane raft GO:0045121 IDA
 Experiment description of studies that identified TLR2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for TLR2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-Western Homo sapiens
2 CD14 929
Affinity Capture-Western Homo sapiens
3 RIPK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 AP1M1 8907
Affinity Capture-MS Homo sapiens
5 LGR4 55366
Affinity Capture-MS Homo sapiens
6 TMEM214 54867
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MYD88 4615
Two-hybrid Homo sapiens
8 TOLLIP 54472
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 STAT5A 6776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ACVR2A  
Affinity Capture-MS Homo sapiens
11 TRIM58 25893
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 IRAK1  
Affinity Capture-Western Homo sapiens
13 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
14 BMPR1A 657
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CLEC7A  
Affinity Capture-Western Homo sapiens
16 CREBBP  
Affinity Capture-Western Homo sapiens
17 ITPRIP  
Affinity Capture-MS Homo sapiens
18 TLR1 7096
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 BLK 640
Affinity Capture-MS Homo sapiens
20 CLEC4M  
Affinity Capture-Western Homo sapiens
21 LY96  
Affinity Capture-Western Homo sapiens
22 TLR2 7097
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 TIRAP  
PCA Homo sapiens
PCA Homo sapiens
24 STK11IP 114790
Affinity Capture-MS Homo sapiens
25 IRF3 3661
Affinity Capture-Western Homo sapiens
26 USP33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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