Experiment description of studies that identified DKK3 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

5 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for DKK3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RECQL4   Affinity Capture-MS Homo sapiens 2 SENP5   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 FOXF1 2294 Affinity Capture-MS Homo sapiens 4 ATP5H 10476 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 INHBE 83729 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 SLC25A16   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 HS3ST1   Affinity Capture-MS Homo sapiens 8 CEP131 22994 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 SLC25A6 293 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 NPW   Affinity Capture-MS Homo sapiens 11 MRPS12   Affinity Capture-MS Homo sapiens 12 GSK3B 2932 Affinity Capture-MS Homo sapiens 13 ALMS1   Affinity Capture-MS Homo sapiens 14 CHST12 55501 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 GTPBP6   Affinity Capture-MS Homo sapiens 16 NME4 4833 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 VGF   Affinity Capture-MS Homo sapiens 18 ATAD3B 83858 Affinity Capture-MS Homo sapiens 19 WNT7A   Affinity Capture-MS Homo sapiens 20 ASPM 259266 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 UBA52 7311 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 ATP5F1 515 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 IFFO1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 POM121   Affinity Capture-MS Homo sapiens 25 ATAD3C 219293 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 GOSR2 9570 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 MYO5A 4644 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 PLEKHH1   Affinity Capture-MS Homo sapiens 29 TTF2 8458 Affinity Capture-MS Homo sapiens 30 MAP7D1 55700 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 MYO5C 55930 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 TNIP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 GORASP1 64689 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 RPS3 6188 Affinity Capture-MS Homo sapiens 35 GOLGA2 2801 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 LONP2 83752 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 MYO1D 4642 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 SULF2 55959 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 SREBF2 6721 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 GLG1 2734 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 GDF11 10220 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 PCOLCE2 26577 Affinity Capture-MS Homo sapiens 43 MYO5B 4645 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 PCM1 5108 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 GFPT2 9945 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 SENP1   Affinity Capture-MS Homo sapiens 47 TIMP3 7078 Affinity Capture-MS Homo sapiens 48 MYO9B 4650 Affinity Capture-MS Homo sapiens 49 LAMA5 3911 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 50 UBQLN4 56893 Two-hybrid Homo sapiens 51 MYO9A 4649 Affinity Capture-MS Homo sapiens 52 ABCB10 23456 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 ADAMTS1 9510 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 54 STX5 6811 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 HSPD1 3329 Affinity Capture-MS Homo sapiens 56 CHSY1 22856 Affinity Capture-MS Homo sapiens 57 DNAJC30   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 58 ATP8   Affinity Capture-MS Homo sapiens 59 ATP5O 539 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 SMOC1 64093 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 61 MYO1B 4430 Affinity Capture-MS Homo sapiens 62 SLC25A5 292 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which DKK3 is involved

No pathways found