Gene description for NFS1
Gene name NFS1 cysteine desulfurase
Gene symbol NFS1
Other names/aliases HUSSY-08
IscS
NIFS
Species Homo sapiens
 Database cross references - NFS1
ExoCarta ExoCarta_9054
Vesiclepedia VP_9054
Entrez Gene 9054
HGNC 15910
MIM 603485
UniProt Q9Y697  
 NFS1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NFS1
Molecular Function
    protein binding GO:0005515 IPI
    pyridoxal phosphate binding GO:0030170 IEA
    cysteine desulfurase activity GO:0031071 EXP
    cysteine desulfurase activity GO:0031071 IBA
    cysteine desulfurase activity GO:0031071 IDA
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    iron-sulfur cluster binding GO:0051536 IEA
Biological Process
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IEA
    iron-sulfur cluster assembly GO:0016226 IBA
    iron-sulfur cluster assembly GO:0016226 NAS
    iron-sulfur cluster assembly GO:0016226 TAS
    iron incorporation into metallo-sulfur cluster GO:0018283 IDA
    molybdopterin cofactor metabolic process GO:0043545 IDA
    [2Fe-2S] cluster assembly GO:0044571 IDA
    [4Fe-4S] cluster assembly GO:0044572 IDA
    [4Fe-4S] cluster assembly GO:0044572 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 NAS
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    mitochondrial [2Fe-2S] assembly complex GO:0099128 IDA
    iron-sulfur cluster assembly complex GO:1990229 NAS
 Experiment description of studies that identified NFS1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NFS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 SLIRP 81892
Proximity Label-MS Homo sapiens
3 PHC1  
Affinity Capture-MS Homo sapiens
4 USP5 8078
Co-fractionation Homo sapiens
5 TRUB2  
Proximity Label-MS Homo sapiens
6 MTG2  
Proximity Label-MS Homo sapiens
7 NGRN  
Proximity Label-MS Homo sapiens
8 CHCHD1  
Proximity Label-MS Homo sapiens
9 BPNT1 10380
Affinity Capture-MS Homo sapiens
10 RPUSD4 84881
Proximity Label-MS Homo sapiens
11 ACADM 34
Co-fractionation Homo sapiens
12 C15orf48  
Affinity Capture-MS Homo sapiens
13 DNAJC15  
Proximity Label-MS Homo sapiens
14 MTRF1  
Proximity Label-MS Homo sapiens
15 GFM2 84340
Proximity Label-MS Homo sapiens
16 CCDC90B  
Proximity Label-MS Homo sapiens
17 PCGF2 7703
Affinity Capture-MS Homo sapiens
18 LRPPRC 10128
Proximity Label-MS Homo sapiens
19 CRYZ 1429
Proximity Label-MS Homo sapiens
20 TFAM 7019
Proximity Label-MS Homo sapiens
21 PMPCA 23203
Proximity Label-MS Homo sapiens
22 COQ9  
Affinity Capture-MS Homo sapiens
23 C8orf82  
Proximity Label-MS Homo sapiens
24 HINT2 84681
Proximity Label-MS Homo sapiens
25 COQ3  
Affinity Capture-MS Homo sapiens
26 OXT  
Affinity Capture-MS Homo sapiens
27 PSMC2 5701
Co-fractionation Homo sapiens
28 MRPS26 64949
Proximity Label-MS Homo sapiens
29 EXD2  
Proximity Label-MS Homo sapiens
30 BCOR  
Affinity Capture-MS Homo sapiens
31 NDUFAB1 4706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ZWINT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PMPCB 9512
Proximity Label-MS Homo sapiens
34 TACO1  
Proximity Label-MS Homo sapiens
35 FASTKD2  
Proximity Label-MS Homo sapiens
36 VWA8 23078
Proximity Label-MS Homo sapiens
37 SOD1 6647
Affinity Capture-MS Homo sapiens
38 MTERF3  
Proximity Label-MS Homo sapiens
39 HSPA8 3312
Affinity Capture-MS Homo sapiens
40 MDH2 4191
Proximity Label-MS Homo sapiens
41 DLD 1738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 C6orf203  
Proximity Label-MS Homo sapiens
43 FASTKD5  
Proximity Label-MS Homo sapiens
44 ISCU 23479
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 PARK2  
Affinity Capture-MS Homo sapiens
46 TUFM 7284
Proximity Label-MS Homo sapiens
47 HSCB 150274
Proximity Label-MS Homo sapiens
48 CCDC109B 55013
Proximity Label-MS Homo sapiens
49 GPSM3  
Affinity Capture-MS Homo sapiens
50 TBRG4 9238
Proximity Label-MS Homo sapiens
51 LYRM4 57128
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MTFMT  
Proximity Label-MS Homo sapiens
53 CTR9 9646
Affinity Capture-MS Homo sapiens
54 MRPL11 65003
Proximity Label-MS Homo sapiens
55 MTRF1L  
Proximity Label-MS Homo sapiens
56 HDAC5 10014
Affinity Capture-MS Homo sapiens
57 TMEM70  
Proximity Label-MS Homo sapiens
58 BIN1 274
Co-fractionation Homo sapiens
59 COX4I1 1327
Proximity Label-MS Homo sapiens
60 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 OTC 5009
Proximity Label-MS Homo sapiens
63 MRRF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
64 AIFM1 9131
Proximity Label-MS Homo sapiens
65 MRPS12  
Proximity Label-MS Homo sapiens
66 MTIF2 4528
Proximity Label-MS Homo sapiens
67 C9orf78 51759
Affinity Capture-MS Homo sapiens
68 MTIF3  
Proximity Label-MS Homo sapiens
69 CARTPT  
Affinity Capture-MS Homo sapiens
70 RMND1 55005
Proximity Label-MS Homo sapiens
71 WIF1 11197
Affinity Capture-MS Homo sapiens
72 ABCC1 4363
Co-fractionation Homo sapiens
73 IKZF1  
Affinity Capture-MS Homo sapiens
74 C12orf65  
Proximity Label-MS Homo sapiens
75 MTG1  
Proximity Label-MS Homo sapiens
76 NDUFA4 4697
Affinity Capture-MS Homo sapiens
77 PPP2R1A 5518
Co-fractionation Homo sapiens
78 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
79 FBXO2 26232
Affinity Capture-MS Homo sapiens
80 CLEC4M  
Affinity Capture-MS Homo sapiens
81 ACAD9 28976
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 KRAS 3845
Negative Genetic Homo sapiens
83 METTL15  
Proximity Label-MS Homo sapiens
84 TACC3  
Affinity Capture-MS Homo sapiens
85 CLPP 8192
Proximity Label-MS Homo sapiens
86 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 TSFM 10102
Proximity Label-MS Homo sapiens
89 AUH 549
Proximity Label-MS Homo sapiens
90 DNAJB6 10049
Affinity Capture-MS Homo sapiens
91 ICT1 3396
Proximity Label-MS Homo sapiens
92 LONP1 9361
Proximity Label-MS Homo sapiens
93 ABHD10 55347
Affinity Capture-MS Homo sapiens
94 EFCAB13  
Affinity Capture-MS Homo sapiens
95 MEN1 4221
Affinity Capture-MS Homo sapiens
96 PDHA1 5160
Proximity Label-MS Homo sapiens
97 C17orf89  
Affinity Capture-MS Homo sapiens
98 COX8A  
Proximity Label-MS Homo sapiens
99 SUCLA2 8803
Co-fractionation Homo sapiens
100 C21orf33  
Proximity Label-MS Homo sapiens
101 CDNF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 SSBP1 6742
Proximity Label-MS Homo sapiens
103 ESR1  
Affinity Capture-MS Homo sapiens
104 GFM1 85476
Proximity Label-MS Homo sapiens
105 AARS2  
Proximity Label-MS Homo sapiens
106 TEFM  
Proximity Label-MS Homo sapiens
107 RPUSD3  
Proximity Label-MS Homo sapiens
108 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 C1QBP 708
Proximity Label-MS Homo sapiens
110 SERBP1 26135
Affinity Capture-MS Homo sapiens
111 OXCT2  
Affinity Capture-MS Homo sapiens
112 METTL17  
Proximity Label-MS Homo sapiens
113 GPX7 2882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 N6AMT2 221143
Affinity Capture-MS Homo sapiens
115 DDX28  
Proximity Label-MS Homo sapiens
116 EP300 2033
Affinity Capture-MS Homo sapiens
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