Gene description for ADPRHL2
Gene name ADP-ribosylhydrolase like 2
Gene symbol ADPRHL2
Other names/aliases ARH3
Species Homo sapiens
 Database cross references - ADPRHL2
ExoCarta ExoCarta_54936
Vesiclepedia VP_54936
Entrez Gene 54936
HGNC 21304
MIM 610624
UniProt Q9NX46  
 ADPRHL2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ADPRHL2
Molecular Function
    magnesium ion binding GO:0000287 IDA
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IDA
    poly(ADP-ribose) glycohydrolase activity GO:0004649 IDA
    poly(ADP-ribose) glycohydrolase activity GO:0004649 IMP
    poly(ADP-ribose) glycohydrolase activity GO:0004649 TAS
    protein binding GO:0005515 IPI
    O-acetyl-ADP-ribose deacetylase activity GO:0061463 IDA
    ADP-ribosylserine hydrolase activity GO:0140292 IDA
Biological Process
    DNA repair GO:0006281 IMP
    base-excision repair, gap-filling GO:0006287 TAS
    negative regulation of necroptotic process GO:0060546 ISS
    cellular response to superoxide GO:0071451 IMP
    peptidyl-serine ADP-deribosylation GO:0140290 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    nuclear body GO:0016604 IDA
    site of DNA damage GO:0090734 IDA
 Experiment description of studies that identified ADPRHL2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADPRHL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFAB1 4706
Co-fractionation Homo sapiens
2 CLIC1 1192
Co-fractionation Homo sapiens
3 GLOD4 51031
Co-fractionation Homo sapiens
4 SOD1 6647
Co-fractionation Homo sapiens
5 GPI 2821
Co-fractionation Homo sapiens
6 PRNP 5621
Affinity Capture-MS Homo sapiens
7 GCSH 2653
Co-fractionation Homo sapiens
8 RIN3  
Affinity Capture-MS Homo sapiens
9 HSPB1 3315
Two-hybrid Homo sapiens
10 FCGR2A 2212
Affinity Capture-MS Homo sapiens
11 TNKS 8658
Two-hybrid Homo sapiens
12 NCBP2 22916
Co-fractionation Homo sapiens
13 PRDM5  
Two-hybrid Homo sapiens
14 FDX1 2230
Co-fractionation Homo sapiens
15 PCID2 55795
Co-fractionation Homo sapiens
16 IGSF8 93185
Affinity Capture-MS Homo sapiens
17 FRA10AC1 118924
Two-hybrid Homo sapiens
18 ACO2 50
Co-fractionation Homo sapiens
19 IMPDH2 3615
Co-fractionation Homo sapiens
20 HEXDC  
Two-hybrid Homo sapiens
21 ERC1 23085
Co-fractionation Homo sapiens
22 PGM1 5236
Co-fractionation Homo sapiens
23 NEUROD4  
Affinity Capture-MS Homo sapiens
24 RAD54L2  
Affinity Capture-MS Homo sapiens
25 FEN1 2237
Co-fractionation Homo sapiens
26 FDX1L  
Co-fractionation Homo sapiens
27 BZW1 9689
Co-fractionation Homo sapiens
View the network image/svg+xml



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