Gene description for PCID2
Gene name PCI domain containing 2
Gene symbol PCID2
Other names/aliases F10
Species Homo sapiens
 Database cross references - PCID2
ExoCarta ExoCarta_55795
Vesiclepedia VP_55795
Entrez Gene 55795
HGNC 25653
MIM 613713
UniProt Q5JVF3  
 PCID2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Squamous carcinoma cells 20124223    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for PCID2
Molecular Function
    double-stranded DNA binding GO:0003690 IBA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
Biological Process
    post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery GO:0000973 IBA
    transcription elongation by RNA polymerase II GO:0006368 IBA
    protein transport GO:0015031 IEA
    poly(A)+ mRNA export from nucleus GO:0016973 IBA
    negative regulation of apoptotic process GO:0043066 IMP
    regulation of mRNA stability GO:0043488 IMP
    positive regulation of B cell differentiation GO:0045579 IEA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    spleen development GO:0048536 ISS
    negative regulation of transcription initiation by RNA polymerase II GO:0060633 ISS
    heterochromatin organization GO:0070828 ISS
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 IMP
    negative regulation of lymphoid progenitor cell differentiation GO:1905457 ISS
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nuclear pore nuclear basket GO:0044615 IDA
    transcription export complex 2 GO:0070390 IBA
    transcription export complex 2 GO:0070390 IDA
 Experiment description of studies that identified PCID2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCID2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 HECTD1 25831
Affinity Capture-MS Homo sapiens
4 CETN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 VPS35 55737
Co-fractionation Homo sapiens
6 UBE2H 7328
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 MYCN  
Affinity Capture-MS Homo sapiens
9 UBE4B 10277
Co-fractionation Homo sapiens
10 PARP1 142
Proximity Label-MS Homo sapiens
11 IMPDH2 3615
Co-fractionation Homo sapiens
12 COIL  
Proximity Label-MS Homo sapiens
13 LSM1 27257
Co-fractionation Homo sapiens
14 PSMD6 9861
Co-fractionation Homo sapiens
15 TP53 7157
Negative Genetic Homo sapiens
16 CIT 11113
Affinity Capture-MS Homo sapiens
17 TMEM63B 55362
Affinity Capture-MS Homo sapiens
18 NEK6 10783
Affinity Capture-MS Homo sapiens
19 PPIE 10450
Affinity Capture-MS Homo sapiens
20 IKZF3  
Two-hybrid Homo sapiens
21 DDX3X 1654
Co-fractionation Homo sapiens
22 TOLLIP 54472
Proximity Label-MS Homo sapiens
23 TRIM66  
Affinity Capture-MS Homo sapiens
24 MEN1 4221
Affinity Capture-MS Homo sapiens
25 SNX2 6643
Co-fractionation Homo sapiens
26 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
27 SHFM1 7979
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
28 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TTC4  
Co-fractionation Homo sapiens
30 UBC 7316
Affinity Capture-MS Homo sapiens
31 EGFR 1956
Negative Genetic Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 C7orf76  
Affinity Capture-MS Homo sapiens
34 SHMT2 6472
Affinity Capture-RNA Homo sapiens
35 ADPRHL2 54936
Co-fractionation Homo sapiens
36 SNX6 58533
Co-fractionation Homo sapiens
37 NXF1 10482
Affinity Capture-RNA Homo sapiens
38 C9orf78 51759
Affinity Capture-MS Homo sapiens
39 MOV10 4343
Affinity Capture-RNA Homo sapiens
40 MYC  
Affinity Capture-MS Homo sapiens
41 KIF11 3832
Affinity Capture-MS Homo sapiens
42 NIN 51199
Proximity Label-MS Homo sapiens
43 RAD18  
Affinity Capture-MS Homo sapiens
44 BRD1 23774
Affinity Capture-MS Homo sapiens
45 SMAD2 4087
Affinity Capture-MS Homo sapiens
46 Tnpo1 238799
Affinity Capture-MS Mus musculus
47 EID1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
48 DDRGK1 65992
Affinity Capture-MS Homo sapiens
49 PML 5371
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
50 C9orf72  
Affinity Capture-MS Homo sapiens
51 KRAS 3845
Negative Genetic Homo sapiens
52 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
53 DDX39B 7919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 LENG8  
Two-hybrid Homo sapiens
56 CAPZB 832
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which PCID2 is involved
No pathways found





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