Gene description for HTRA2
Gene name HtrA serine peptidase 2
Gene symbol HTRA2
Other names/aliases OMI
PARK13
PRSS25
Species Homo sapiens
 Database cross references - HTRA2
ExoCarta ExoCarta_27429
Vesiclepedia VP_27429
Entrez Gene 27429
HGNC 14348
MIM 606441
UniProt O43464  
 HTRA2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for HTRA2
Molecular Function
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 IMP
    serine-type endopeptidase activity GO:0004252 TAS
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    peptidase activity GO:0008233 IMP
    serine-type peptidase activity GO:0008236 IDA
    serine-type peptidase activity GO:0008236 TAS
    protein serine/threonine kinase inhibitor activity GO:0030291 IDA
    identical protein binding GO:0042802 IPI
    unfolded protein binding GO:0051082 NAS
    ubiquitin ligase inhibitor activity GO:1990948 IDA
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IMP
    proteolysis GO:0006508 TAS
    ceramide metabolic process GO:0006672 IEA
    mitochondrion organization GO:0007005 IMP
    adult walking behavior GO:0007628 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IMP
    response to herbicide GO:0009635 IEA
    programmed cell death GO:0012501 IBA
    programmed cell death GO:0012501 IDA
    protein autoprocessing GO:0016540 TAS
    pentacyclic triterpenoid metabolic process GO:0019742 IEA
    protein catabolic process GO:0030163 IDA
    forebrain development GO:0030900 IEA
    cellular response to oxidative stress GO:0034599 IMP
    cellular response to oxidative stress GO:0034599 NAS
    cellular response to heat GO:0034605 IDA
    cellular response to interferon-beta GO:0035458 IDA
    intracellular signal transduction GO:0035556 IDA
    mitochondrial protein catabolic process GO:0035694 TAS
    regulation of multicellular organism growth GO:0040014 IEA
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of apoptotic process GO:0043065 TAS
    negative regulation of neuron apoptotic process GO:0043524 TAS
    negative regulation of cell cycle GO:0045786 TAS
    neuron development GO:0048666 IEA
    neuron apoptotic process GO:0051402 IEA
    cellular response to retinoic acid GO:0071300 IDA
    cellular response to growth factor stimulus GO:0071363 IMP
    execution phase of apoptosis GO:0097194 TAS
    positive regulation of execution phase of apoptosis GO:1900119 IDA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 NAS
    regulation of autophagy of mitochondrion GO:1903146 TAS
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
    negative regulation of type 2 mitophagy GO:1905090 IEA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IMP
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrion GO:0005739 TAS
    mitochondrial intermembrane space GO:0005758 IDA
    mitochondrial intermembrane space GO:0005758 TAS
    endoplasmic reticulum GO:0005783 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 IDA
    cytoskeleton GO:0005856 IDA
    cytoplasmic side of plasma membrane GO:0009898 ISS
    membrane GO:0016020 IDA
    mitochondrial membrane GO:0031966 IEA
    CD40 receptor complex GO:0035631 ISS
    serine-type endopeptidase complex GO:1905370 IMP
 Experiment description of studies that identified HTRA2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HTRA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
3 XIAP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
4 CAP1 10487
Co-fractionation Homo sapiens
5 APC  
Reconstituted Complex Homo sapiens
6 MAPK13 5603
Biochemical Activity Homo sapiens
7 FDXR 2232
Co-fractionation Homo sapiens
8 ECI1 1632
Co-fractionation Homo sapiens
9 PIR 8544
Co-fractionation Homo sapiens
10 BIRC3 330
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 WDR26 80232
Affinity Capture-MS Homo sapiens
12 KRTAP13-2  
Affinity Capture-MS Homo sapiens
13 HPDL 84842
Co-fractionation Homo sapiens
14 ALDH1A2 8854
Protein-peptide Homo sapiens
15 TRIM28 10155
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
16 NDUFS3 4722
Affinity Capture-MS Homo sapiens
17 SUCLA2 8803
Co-fractionation Homo sapiens
18 RANBP10 57610
Affinity Capture-MS Homo sapiens
19 MDH1 4190
Co-fractionation Homo sapiens
20 MAPK14 1432
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 MKLN1  
Affinity Capture-MS Homo sapiens
22 SLC25A12 8604
Proximity Label-MS Homo sapiens
23 CPA4 51200
Co-fractionation Homo sapiens
24 BNIP3 664
Affinity Capture-MS Homo sapiens
25 VPS4B 9525
Protein-peptide Homo sapiens
26 NDUFV1 4723
Co-fractionation Homo sapiens
27 LATS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
28 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 USP7 7874
Two-hybrid Homo sapiens
30 HSD17B10 3028
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
31 NFU1 27247
Co-fractionation Homo sapiens
32 PSME3 10197
Affinity Capture-MS Homo sapiens
33 DLD 1738
Co-fractionation Homo sapiens
34 MAPK3 5595
Biochemical Activity Homo sapiens
35 MAPK12  
Biochemical Activity Homo sapiens
36 PARK2  
Affinity Capture-Western Homo sapiens
37 CDK5 1020
Biochemical Activity Homo sapiens
38 CCAR2 57805
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
39 ARMC8 25852
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 GDF15 9518
Affinity Capture-MS Homo sapiens
41 DIABLO 56616
Proximity Label-MS Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 ACTA1 58
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
43 PDXDC1 23042
Protein-peptide Homo sapiens
44 C12orf49  
Negative Genetic Homo sapiens
45 GID8 54994
Affinity Capture-MS Homo sapiens
46 TIMM9  
Co-fractionation Homo sapiens
47 SREBF2 6721
Negative Genetic Homo sapiens
48 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 OPA1 4976
Co-fractionation Homo sapiens
50 POLDIP2 26073
Co-fractionation Homo sapiens
51 ARRDC3 57561
Affinity Capture-MS Homo sapiens
52 PAK7  
Affinity Capture-MS Homo sapiens
53 RMND5A 64795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 VCP 7415
Affinity Capture-MS Homo sapiens
55 BIRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
56 CD40 958
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
57 TUBB 203068
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
58 KHSRP 8570
Co-fractionation Homo sapiens
59 HTRA2 27429
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
60 PITRM1 10531
Co-fractionation Homo sapiens
61 EIF4G1 1981
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
62 CSN2 1447
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
63 GOT1 2805
Co-fractionation Homo sapiens
64 CUL3 8452
Affinity Capture-MS Homo sapiens
65 BNIP3L  
Affinity Capture-MS Homo sapiens
66 MYO19  
Affinity Capture-MS Homo sapiens
67 MMP13  
Affinity Capture-MS Homo sapiens
68 BIRC6 57448
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
FRET Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
69 C12orf74  
Affinity Capture-MS Homo sapiens
70 SOD2 6648
Co-fractionation Homo sapiens
71 DSTN 11034
Co-fractionation Homo sapiens
72 KRTAP6-1  
Affinity Capture-MS Homo sapiens
73 TUBB7P 56604
Protein-peptide Homo sapiens
74 HAX1  
Affinity Capture-Western Homo sapiens
75 BIRC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 MRPL18 29074
Affinity Capture-MS Homo sapiens
77 NADK2 133686
Co-fractionation Homo sapiens
78 EEF1A1 1915
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
79 ZRANB1 54764
Affinity Capture-MS Homo sapiens
80 CS 1431
Co-fractionation Homo sapiens
81 KRTAP13-3  
Affinity Capture-MS Homo sapiens
82 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
83 FASN 2194
Positive Genetic Homo sapiens
84 MUL1  
Biochemical Activity Homo sapiens
85 TRAF2 7186
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
86 TIA1 7072
Co-fractionation Homo sapiens
87 CUTA 51596
Co-fractionation Homo sapiens
88 PDCD6IP 10015
Co-fractionation Homo sapiens
89 ABHD10 55347
Co-fractionation Homo sapiens
90 ASS1 445
Co-fractionation Homo sapiens
91 BCL10  
Affinity Capture-MS Homo sapiens
92 MTHFD1 4522
Co-fractionation Homo sapiens
93 CSN2  
Biochemical Activity Bos taurus
94 CDK1 983
Biochemical Activity Homo sapiens
95 TUBB6 84617
Protein-peptide Homo sapiens
96 OAT 4942
Co-fractionation Homo sapiens
97 TUBA1A 7846
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
98 PDXDC2P  
Biochemical Activity Homo sapiens
99 CAD 790
Protein-peptide Homo sapiens
100 PINK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
101 MAPK11  
Biochemical Activity Homo sapiens
102 SSSCA1 10534
Affinity Capture-MS Homo sapiens
103 REXO2 25996
Co-fractionation Homo sapiens
104 LDLR 3949
Positive Genetic Homo sapiens
105 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
106 C9orf72  
Affinity Capture-MS Homo sapiens
107 BIRC7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 VIM 7431
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
109 NDUFS6  
Affinity Capture-MS Homo sapiens
110 MAEA 10296
Affinity Capture-MS Homo sapiens
111 TRAF1 7185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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