Experiment description of studies that identified SFT2D2 in sEVs

1 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for SFT2D2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 IFNGR2   Two-hybrid Homo sapiens 2 SLC30A8   Two-hybrid Homo sapiens 3 TMEM51 55092 Two-hybrid Homo sapiens 4 SLC10A6   Two-hybrid Homo sapiens 5 TAS2R5   Two-hybrid Homo sapiens 6 TMEM143   Two-hybrid Homo sapiens 7 ATP5J 522 Two-hybrid Homo sapiens 8 RPS2 6187 Cross-Linking-MS (XL-MS) Homo sapiens 9 GOLT1B 51026 Affinity Capture-MS Homo sapiens 10 TCP11X2   Two-hybrid Homo sapiens 11 TMEM14B   Two-hybrid Homo sapiens 12 HSD17B11 51170 Two-hybrid Homo sapiens 13 HSP90AB1 3326 Cross-Linking-MS (XL-MS) Homo sapiens 14 LAMP2 3920 Proximity Label-MS Homo sapiens 15 REEP4   Two-hybrid Homo sapiens 16 REEP2   Two-hybrid Homo sapiens 17 STOM 2040 Two-hybrid Homo sapiens 18 PBX3   Two-hybrid Homo sapiens 19 GPR152   Two-hybrid Homo sapiens 20 INPP5K 51763 Two-hybrid Homo sapiens 21 NDRG4 65009 Two-hybrid Homo sapiens 22 RAB2A 5862 Proximity Label-MS Homo sapiens 23 HSD17B13   Two-hybrid Homo sapiens 24 HIBADH 11112 Two-hybrid Homo sapiens 25 SLC10A1   Two-hybrid Homo sapiens 26 BNIP2   Two-hybrid Homo sapiens 27 PLIN3 10226 Two-hybrid Homo sapiens 28 CREB3   Two-hybrid Homo sapiens 29 MOB2 81532 Two-hybrid Homo sapiens 30 RAB5C 5878 Proximity Label-MS Homo sapiens 31 PLIN2 123 Two-hybrid Homo sapiens 32 ELOVL4   Two-hybrid Homo sapiens 33 TIMM44 10469 Two-hybrid Homo sapiens View the network

Pathways in which SFT2D2 is involved

No pathways found