Gene description for MECR
Gene name mitochondrial trans-2-enoyl-CoA reductase
Gene symbol MECR
Other names/aliases CGI-63
FASN2B
NRBF1
Species Homo sapiens
 Database cross references - MECR
ExoCarta ExoCarta_51102
Vesiclepedia VP_51102
Entrez Gene 51102
HGNC 19691
MIM 608205
UniProt Q9BV79  
 MECR identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MECR
Molecular Function
    enoyl-[acyl-carrier-protein] reductase (NADPH) activity GO:0141148 IDA
Biological Process
    fatty acid metabolic process GO:0006631 IBA
    fatty acid metabolic process GO:0006631 IDA
    fatty acid metabolic process GO:0006631 IMP
    fatty acid biosynthetic process GO:0006633 IEA
    intracellular iron ion homeostasis GO:0006879 IMP
    ceramide biosynthetic process GO:0046513 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    membrane GO:0016020 IEA
 Experiment description of studies that identified MECR in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MECR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RARA 5914
Two-hybrid Homo sapiens
2 EEF1G 1937
Cross-Linking-MS (XL-MS) Homo sapiens
3 TAS2R41  
Affinity Capture-MS Homo sapiens
4 RAB7A 7879
Co-fractionation Homo sapiens
5 WASF2 10163
Affinity Capture-MS Homo sapiens
6 NCKAP1 10787
Affinity Capture-MS Homo sapiens
7 AURKA 6790
Affinity Capture-MS Homo sapiens
8 LAMP1 3916
Affinity Capture-MS Homo sapiens
9 ATP6AP2 10159
Co-fractionation Homo sapiens
10 Rxra  
Two-hybrid Mus musculus
11 DMRT1  
Affinity Capture-MS Homo sapiens
12 S100A13 6284
Cross-Linking-MS (XL-MS) Homo sapiens
13 BIRC6 57448
Affinity Capture-MS Homo sapiens
14 MOV10 4343
Affinity Capture-RNA Homo sapiens
15 PPARA  
Reconstituted Complex Homo sapiens
16 EGFR 1956
Negative Genetic Homo sapiens
17 Ppara  
Two-hybrid Mus musculus
18 SNX30 401548
Affinity Capture-MS Homo sapiens
19 NR2E1  
Affinity Capture-MS Homo sapiens
20 PPA2 27068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 KRAS 3845
Synthetic Lethality Homo sapiens
22 Yap1  
Affinity Capture-MS Mus musculus
23 RNF133  
Affinity Capture-MS Homo sapiens
24 Thrb  
Two-hybrid Rattus norvegicus
25 DBT 1629
Affinity Capture-MS Homo sapiens
26 Hnf4a  
Two-hybrid Rattus norvegicus
27 MTRF1L  
Co-fractionation Homo sapiens
28 SNX4 8723
Affinity Capture-MS Homo sapiens
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