Gene description for SPRY2
Gene name sprouty homolog 2 (Drosophila)
Gene symbol SPRY2
Other names/aliases hSPRY2
Species Homo sapiens
 Database cross references - SPRY2
ExoCarta ExoCarta_10253
Vesiclepedia VP_10253
Entrez Gene 10253
HGNC 11270
MIM 602466
UniProt O43597  
 SPRY2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SPRY2
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    protein serine/threonine kinase inhibitor activity GO:0030291 IC
    protein serine/threonine kinase activator activity GO:0043539 IMP
    ubiquitin-protein transferase inhibitor activity GO:0055105 IEA
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IEA
    sensory perception of sound GO:0007605 IEA
    negative regulation of cell population proliferation GO:0008285 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of epithelial to mesenchymal transition GO:0010719 ISS
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IDA
    negative regulation of angiogenesis GO:0016525 IMP
    positive regulation of cell migration GO:0030335 IMP
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 ISS
    negative regulation of cell projection organization GO:0031345 ISS
    negative regulation of protein ubiquitination GO:0031397 IDA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IDA
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 IBA
    inner ear morphogenesis GO:0042472 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    cell fate commitment GO:0045165 IEA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IEA
    negative regulation of Ras protein signal transduction GO:0046580 IBA
    animal organ development GO:0048513 IBA
    negative regulation of neurotrophin TRK receptor signaling pathway GO:0051387 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    lung growth GO:0060437 IEA
    bud elongation involved in lung branching GO:0060449 IEA
    ERK1 and ERK2 cascade GO:0070371 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IBA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    negative regulation of vascular endothelial growth factor signaling pathway GO:1900747 IDA
    negative regulation of lens fiber cell differentiation GO:1902747 ISS
    cellular response to leukemia inhibitory factor GO:1990830 IEA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 ISS
    plasma membrane GO:0005886 TAS
    actin cytoskeleton GO:0015629 IDA
    microtubule cytoskeleton GO:0015630 IDA
    membrane GO:0016020 ISS
    ruffle membrane GO:0032587 IEA
    microtubule end GO:1990752 IDA
 Experiment description of studies that identified SPRY2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPRY2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRAP2 9402
Two-hybrid Homo sapiens
2 LCE1F  
Two-hybrid Homo sapiens
3 SNX3 8724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GNPDA2 132789
Affinity Capture-MS Homo sapiens
5 KRTAP10-11  
Two-hybrid Homo sapiens
6 TRIM51  
Two-hybrid Homo sapiens
7 SIAH2 6478
Reconstituted Complex Homo sapiens
8 LCE1B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 MDFI  
Two-hybrid Homo sapiens
10 MAPKBP1  
Two-hybrid Homo sapiens
11 EGLN1 54583
Affinity Capture-Western Homo sapiens
12 VHL  
Affinity Capture-Western Homo sapiens
13 GNE 10020
Two-hybrid Homo sapiens
14 EIF4E2  
Two-hybrid Homo sapiens
15 CAB39 51719
Affinity Capture-MS Homo sapiens
16 CRIP2 1397
Two-hybrid Homo sapiens
17 VANGL2  
Affinity Capture-MS Homo sapiens
18 KRTAP4-7  
Two-hybrid Homo sapiens
19 KRTAP4-12  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 ZNF266  
Two-hybrid Homo sapiens
21 LCE3C  
Two-hybrid Homo sapiens
22 SDHB 6390
Two-hybrid Homo sapiens
23 LASP1 3927
Two-hybrid Homo sapiens
24 HGS 9146
Affinity Capture-Western Homo sapiens
25 LCE2D  
Two-hybrid Homo sapiens
26 CTH 1491
Affinity Capture-MS Homo sapiens
27 NR1H2  
Two-hybrid Homo sapiens
28 MID2 11043
Two-hybrid Homo sapiens
29 KRTAP4-11  
Two-hybrid Homo sapiens
30 MLLT6  
Two-hybrid Homo sapiens
31 CRMP1 1400
Two-hybrid Homo sapiens
32 CATSPER1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 PRKCD 5580
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
34 NCK2 8440
Two-hybrid Homo sapiens
35 NEDD4 4734
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 TES 26136
Affinity Capture-MS Homo sapiens
37 LCE1A  
Two-hybrid Homo sapiens
38 FRS3 10817
Two-hybrid Homo sapiens
39 KRTAP5-6  
Two-hybrid Homo sapiens
40 CAV3 859
Affinity Capture-MS Homo sapiens
41 ADAMTSL4  
Two-hybrid Homo sapiens
42 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 LONRF1  
Two-hybrid Homo sapiens
44 AQP1 358
Two-hybrid Homo sapiens
45 TMEM14B  
Two-hybrid Homo sapiens
46 PLSCR1 5359
Two-hybrid Homo sapiens
47 RTFDC1  
Two-hybrid Homo sapiens
48 ZBTB16  
Two-hybrid Homo sapiens
49 TESK1 7016
Affinity Capture-Western Homo sapiens
50 KRTAP10-1  
Two-hybrid Homo sapiens
51 MCAM 4162
Proximity Label-MS Homo sapiens
52 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
53 Itsn1 16443
Affinity Capture-Western Mus musculus
54 UBASH3B 84959
Two-hybrid Homo sapiens
55 MB21D2  
Two-hybrid Homo sapiens
56 SPRY4 81848
Affinity Capture-Western Homo sapiens
57 CDC42EP4  
Affinity Capture-MS Homo sapiens
58 GPRIN2  
Two-hybrid Homo sapiens
59 FAIM  
Two-hybrid Homo sapiens
60 LCE3E  
Two-hybrid Homo sapiens
61 PPP2CA 5515
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 MEOX2  
Two-hybrid Homo sapiens
63 LCN1 3933
Affinity Capture-MS Homo sapiens
64 CTSB 1508
Two-hybrid Homo sapiens
65 DNM2 1785
Two-hybrid Homo sapiens
66 CSTB 1476
Two-hybrid Homo sapiens
67 SEMA5B  
Two-hybrid Homo sapiens
68 BAG3 9531
Two-hybrid Homo sapiens
69 HRAS 3265
Affinity Capture-Western Homo sapiens
70 EGLN3  
Affinity Capture-Western Homo sapiens
71 KRTAP10-8  
Two-hybrid Homo sapiens
72 C19orf66  
Two-hybrid Homo sapiens
73 HSD3B7 80270
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
74 PRKD1 5587
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
75 ATRIP  
Two-hybrid Homo sapiens
76 PACSIN3 29763
Two-hybrid Homo sapiens
77 KRTAP10-3  
Two-hybrid Homo sapiens
78 HOXA1 3198
Two-hybrid Homo sapiens
79 KRTAP5-9  
Two-hybrid Homo sapiens
80 OTX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
81 Hoxa1  
Two-hybrid Mus musculus
82 RUNX1T1  
Two-hybrid Homo sapiens
83 UBR1 197131
Two-hybrid Homo sapiens
84 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
85 UBASH3A 53347
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
86 CHRD  
Two-hybrid Homo sapiens
87 ZNF587  
Two-hybrid Homo sapiens
88 SPRY1 10252
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
89 DGCR6  
Two-hybrid Homo sapiens
90 NUBP1 4682
Two-hybrid Homo sapiens
91 RAF1 5894
Affinity Capture-Western Homo sapiens
92 KRTAP9-2  
Two-hybrid Homo sapiens
93 LCE4A  
Two-hybrid Homo sapiens
94 TXNDC5 81567
Two-hybrid Homo sapiens
95 PRKAB2 5565
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
96 S100A13 6284
Affinity Capture-MS Homo sapiens
97 ZNF417  
Two-hybrid Homo sapiens
98 ZFP36  
Affinity Capture-RNA Homo sapiens
99 KRTAP10-5  
Two-hybrid Homo sapiens
100 LCE1C  
Two-hybrid Homo sapiens
101 VPS25 84313
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
102 HIF1A 3091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
103 VPS36 51028
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
104 FBN1 2200
Two-hybrid Homo sapiens
105 KIF1A 547
Two-hybrid Homo sapiens
106 PLS1 5357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 GRN 2896
Two-hybrid Homo sapiens
108 STK16 8576
Two-hybrid Homo sapiens
109 TRAF2 7186
Two-hybrid Homo sapiens
110 ITSN1 6453
Two-hybrid Homo sapiens
111 SPRY2 10253
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
112 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
113 GP9 2815
Two-hybrid Homo sapiens
114 MKNK1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
115 KRTAP9-4  
Two-hybrid Homo sapiens
116 EPHX1 2052
Affinity Capture-MS Homo sapiens
117 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
118 UBE2D2 7322
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 PTGER3  
Two-hybrid Homo sapiens
120 CREB5 9586
Two-hybrid Homo sapiens
121 FAM219A 203259
Two-hybrid Homo sapiens
122 KDM1A 23028
Two-hybrid Homo sapiens
123 HEXIM2  
Two-hybrid Homo sapiens
124 NDUFA2 4695
Affinity Capture-MS Homo sapiens
125 EGLN2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
126 FRS2 10818
Affinity Capture-Western Homo sapiens
127 UBE2E3 10477
Affinity Capture-MS Homo sapiens
128 TCAF1 9747
Two-hybrid Homo sapiens
129 CHRNG  
Two-hybrid Homo sapiens
130 KRAS 3845
Proximity Label-MS Homo sapiens
131 SH3KBP1 30011
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
132 VANGL1 81839
Affinity Capture-MS Homo sapiens
133 ZDHHC13 54503
Affinity Capture-MS Homo sapiens
134 USP20  
Two-hybrid Homo sapiens
135 Ptpn11 19247
PCA Mus musculus
136 KRTAP10-9  
Two-hybrid Homo sapiens
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