Gene description for KRT25
Gene name keratin 25, type I
Gene symbol KRT25
Other names/aliases KRT25A
Species Homo sapiens
 Database cross references - KRT25
ExoCarta ExoCarta_147183
Vesiclepedia VP_147183
Entrez Gene 147183
HGNC 30839
UniProt Q7Z3Z0  
 KRT25 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KRT25
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IDA
    protein heterodimerization activity GO:0046982 IMP
Biological Process
    cytoskeleton organization GO:0007010 IDA
    epithelial cell differentiation GO:0030855 IBA
    hair follicle morphogenesis GO:0031069 IBA
    hair cycle GO:0042633 IDA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    keratin filament GO:0045095 IPI
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT25 in sEVs
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KRT25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT72 140807
Two-hybrid Homo sapiens
2 KRT83  
Two-hybrid Homo sapiens
3 KRT85  
Two-hybrid Homo sapiens
4 KRT4 3851
Two-hybrid Homo sapiens
5 TFCP2 7024
Affinity Capture-MS Homo sapiens
6 KRT76 51350
Two-hybrid Homo sapiens
7 KRT3 3850
Two-hybrid Homo sapiens
8 KRT6B 3854
Two-hybrid Homo sapiens
9 KRT1 3848
Two-hybrid Homo sapiens
10 KRT86  
Two-hybrid Homo sapiens
11 KRT6A 3853
Two-hybrid Homo sapiens
12 KRT8 3856
Two-hybrid Homo sapiens
13 KRT71 112802
Two-hybrid Homo sapiens
14 KRT6C 286887
Two-hybrid Homo sapiens
15 PPL 5493
Two-hybrid Homo sapiens
16 MORN4  
Two-hybrid Homo sapiens
17 KRT74 121391
Two-hybrid Homo sapiens
18 YAP1 10413
Affinity Capture-MS Homo sapiens
19 KRT75 9119
Two-hybrid Homo sapiens
20 HGS 9146
Two-hybrid Homo sapiens
21 KRT2 3849
Two-hybrid Homo sapiens
22 KRT5 3852
Two-hybrid Homo sapiens
23 KRT78 196374
Two-hybrid Homo sapiens
24 KRT79 338785
Two-hybrid Homo sapiens
25 CYLD  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KRT25 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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