Gene description for KRT35
Gene name keratin 35, type I
Gene symbol KRT35
Other names/aliases HA5
Ha-5
KRTHA5
hHa5
Species Homo sapiens
 Database cross references - KRT35
ExoCarta ExoCarta_3886
Vesiclepedia VP_3886
Entrez Gene 3886
HGNC 6453
MIM 602764
UniProt Q92764  
 KRT35 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KRT35
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
Biological Process
    anatomical structure morphogenesis GO:0009653 TAS
    epithelial cell differentiation GO:0030855 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    extracellular space GO:0005615 HDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    intermediate filament GO:0005882 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT35 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KRT35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCRS1  
Two-hybrid Homo sapiens
2 ENKD1  
Two-hybrid Homo sapiens
3 PRR35  
Two-hybrid Homo sapiens
4 KRT6B 3854
Two-hybrid Homo sapiens
5 KRT1 3848
Two-hybrid Homo sapiens
6 FRS3 10817
Two-hybrid Homo sapiens
7 ATG9A 79065
Two-hybrid Homo sapiens
8 KIFC3 3801
Two-hybrid Homo sapiens
9 KRT6A 3853
Two-hybrid Homo sapiens
10 MYO15B 80022
Two-hybrid Homo sapiens
11 EPS15 2060
Affinity Capture-MS Homo sapiens
12 CDC25B 994
Affinity Capture-MS Homo sapiens
13 MAPKBP1  
Two-hybrid Homo sapiens
14 ITIH2 3698
Affinity Capture-MS Homo sapiens
15 CD33 945
Two-hybrid Homo sapiens
16 KRT82 3888
Two-hybrid Homo sapiens
17 HAPLN2  
Two-hybrid Homo sapiens
18 SCNM1  
Two-hybrid Homo sapiens
19 LENG1  
Two-hybrid Homo sapiens
20 HGS 9146
Two-hybrid Homo sapiens
21 SLC43A2 124935
Two-hybrid Homo sapiens
22 OIP5  
Two-hybrid Homo sapiens
23 CTSZ 1522
Two-hybrid Homo sapiens
24 KRT5 3852
Two-hybrid Homo sapiens
25 KRT78 196374
Two-hybrid Homo sapiens
26 KRT72 140807
Two-hybrid Homo sapiens
27 KRT83  
Two-hybrid Homo sapiens
28 KRT8 3856
Two-hybrid Homo sapiens
29 LONRF1  
Two-hybrid Homo sapiens
30 USP15 9958
Affinity Capture-MS Homo sapiens
31 HCK 3055
Two-hybrid Homo sapiens
32 AES 166
Two-hybrid Homo sapiens
33 KRT80 144501
Two-hybrid Homo sapiens
34 DTNB 1838
Two-hybrid Homo sapiens
35 TCHP  
Two-hybrid Homo sapiens
36 KRT75 9119
Two-hybrid Homo sapiens
37 PKN1 5585
Two-hybrid Homo sapiens
38 PRPF18  
Two-hybrid Homo sapiens
39 FGFRL1 53834
Affinity Capture-MS Homo sapiens
40 PRPF31 26121
Two-hybrid Homo sapiens
41 LINC00238  
Two-hybrid Homo sapiens
42 KRT85  
Two-hybrid Homo sapiens
43 ABI3 51225
Two-hybrid Homo sapiens
44 KRT3 3850
Two-hybrid Homo sapiens
45 EFNA4  
Affinity Capture-MS Homo sapiens
46 CCDC185  
Two-hybrid Homo sapiens
47 TAPBPL 55080
Two-hybrid Homo sapiens
48 KCTD9  
Two-hybrid Homo sapiens
49 KRT86  
Two-hybrid Homo sapiens
50 CCDC120  
Two-hybrid Homo sapiens
51 TEKT4  
Two-hybrid Homo sapiens
52 KLHL38  
Two-hybrid Homo sapiens
53 TXLNB  
Two-hybrid Homo sapiens
54 FGF6  
Affinity Capture-MS Homo sapiens
55 KRT4 3851
Two-hybrid Homo sapiens
56 LAMA1 284217
Affinity Capture-MS Homo sapiens
57 HOXA1 3198
Two-hybrid Homo sapiens
58 OTX1  
Two-hybrid Homo sapiens
59 KRT81  
Two-hybrid Homo sapiens
60 UBASH3A 53347
Two-hybrid Homo sapiens
61 TBC1D30 23329
Two-hybrid Homo sapiens
62 TIMP1 7076
Affinity Capture-MS Homo sapiens
63 TUBB7P 56604
Affinity Capture-MS Homo sapiens
64 SHC3  
Two-hybrid Homo sapiens
65 TCEANC  
Two-hybrid Homo sapiens
66 SMYD1  
Two-hybrid Homo sapiens
67 ENTHD2  
Two-hybrid Homo sapiens
68 ZNF417  
Two-hybrid Homo sapiens
69 KRT2 3849
Two-hybrid Homo sapiens
70 EFCAB3 146779
Two-hybrid Homo sapiens
71 BCAS2 10286
Two-hybrid Homo sapiens
72 GRB2 2885
Affinity Capture-MS Homo sapiens
73 C21orf58  
Two-hybrid Homo sapiens
74 DLG3 1741
Affinity Capture-MS Homo sapiens
75 TBC1D21 161514
Two-hybrid Homo sapiens
76 TBC1D7  
Two-hybrid Homo sapiens
77 CCHCR1  
Two-hybrid Homo sapiens
78 KRT77 374454
Two-hybrid Homo sapiens
79 KRT71 112802
Two-hybrid Homo sapiens
80 KRT79 338785
Two-hybrid Homo sapiens
81 KRT6C 286887
Two-hybrid Homo sapiens
82 FAM221B  
Two-hybrid Homo sapiens
83 KRT74 121391
Two-hybrid Homo sapiens
84 POU4F2  
Two-hybrid Homo sapiens
85 C1orf109  
Two-hybrid Homo sapiens
86 SDC4 6385
Affinity Capture-MS Homo sapiens
87 TRIM29 23650
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KRT35 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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