Gene description for RTF1
Gene name Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Gene symbol RTF1
Other names/aliases GTL7
KIAA0252
Species Homo sapiens
 Database cross references - RTF1
ExoCarta ExoCarta_23168
Vesiclepedia VP_23168
Entrez Gene 23168
HGNC 28996
MIM 611633
UniProt Q92541  
 RTF1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RTF1
Molecular Function
    single-stranded DNA binding GO:0003697 IDA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    endodermal cell fate commitment GO:0001711 ISS
    blastocyst growth GO:0001832 IEA
    chromatin organization GO:0006325 IEA
    transcription elongation by RNA polymerase II GO:0006368 IDA
    Wnt signaling pathway GO:0016055 IEA
    stem cell population maintenance GO:0019827 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IEA
    Cdc73/Paf1 complex GO:0016593 IDA
 Experiment description of studies that identified RTF1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RTF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 CDC73  
Affinity Capture-Western Homo sapiens
4 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
5 ARRB1 408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 H2AFJ 55766
Affinity Capture-MS Homo sapiens
7 RPL37 6167
Co-fractionation Homo sapiens
8 NUMA1 4926
Co-fractionation Homo sapiens
9 ARRB2 409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PTPN11 5781
Affinity Capture-Western Homo sapiens
11 PLEKHJ1  
Affinity Capture-MS Homo sapiens
12 CTR9 9646
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
13 PARP1 142
Proximity Label-MS Homo sapiens
14 NEDD4 4734
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 CENPA  
Proximity Label-MS Homo sapiens
16 COIL  
Proximity Label-MS Homo sapiens
17 LEO1 123169
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
18 ZRANB2 9406
Co-fractionation Homo sapiens
19 WDR61 80349
Affinity Capture-Western Homo sapiens
20 BAG2 9532
Affinity Capture-MS Homo sapiens
21 SRPK2 6733
Biochemical Activity Homo sapiens
22 SERF2 10169
Affinity Capture-MS Homo sapiens
23 CDK11B 984
Co-fractionation Homo sapiens
24 EPB41L3 23136
Affinity Capture-MS Homo sapiens
25 FGF12  
Affinity Capture-MS Homo sapiens
26 APEX1 328
Proximity Label-MS Homo sapiens
27 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
28 ACTC1 70
Proximity Label-MS Homo sapiens
29 NOP56 10528
Proximity Label-MS Homo sapiens
30 DHX8 1659
Proximity Label-MS Homo sapiens
31 SUPT5H 6829
Co-fractionation Homo sapiens
32 RPS6 6194
Proximity Label-MS Homo sapiens
33 CSNK2A1 1457
Co-fractionation Homo sapiens
34 ETF1 2107
Co-fractionation Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 HSPA1A 3303
Affinity Capture-MS Homo sapiens
37 MAGEA9  
Affinity Capture-MS Homo sapiens
38 DDX58 23586
Affinity Capture-RNA Homo sapiens
39 SNX6 58533
Co-fractionation Homo sapiens
40 NIFK 84365
Proximity Label-MS Homo sapiens
41 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
42 HADHA 3030
Affinity Capture-MS Homo sapiens
43 DNMT3A 1788
Affinity Capture-MS Homo sapiens
44 CHD1 1105
Co-fractionation Homo sapiens
45 EEF2 1938
Cross-Linking-MS (XL-MS) Homo sapiens
46 TCEA1 6917
Reconstituted Complex Homo sapiens
47 C20orf197  
Affinity Capture-MS Homo sapiens
48 CBX3 11335
Proximity Label-MS Homo sapiens
49 SRRM2 23524
Co-fractionation Homo sapiens
50 DDX23 9416
Proximity Label-MS Homo sapiens
51 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 C12orf49  
Negative Genetic Homo sapiens
53 DNMT3B  
Affinity Capture-MS Homo sapiens
54 STAT1 6772
Co-fractionation Homo sapiens
55 YAP1 10413
Co-fractionation Homo sapiens
56 DENND2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 HSPA2 3306
Affinity Capture-MS Homo sapiens
58 ZC3H18  
Co-fractionation Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 RPL31 6160
Proximity Label-MS Homo sapiens
61 EGFR 1956
Negative Genetic Homo sapiens
62 PDCD6 10016
Affinity Capture-MS Homo sapiens
63 RPL35A 6165
Co-fractionation Homo sapiens
64 PLCD3 113026
Affinity Capture-MS Homo sapiens
65 XRCC6 2547
Proximity Label-MS Homo sapiens
66 UBTF 7343
Co-fractionation Homo sapiens
67 KRAS 3845
Negative Genetic Homo sapiens
68 DHX40  
Proximity Label-MS Homo sapiens
69 HSPH1 10808
Affinity Capture-MS Homo sapiens
70 HADHB 3032
Affinity Capture-MS Homo sapiens
71 PAF1 54623
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
72 SSRP1 6749
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
73 SYT2  
Affinity Capture-MS Homo sapiens
74 GSPT1 2935
Co-fractionation Homo sapiens
75 TRPM8  
Co-fractionation Homo sapiens
76 SUPT16H 11198
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
77 EP300 2033
Affinity Capture-MS Homo sapiens
78 BRD4 23476
Co-fractionation Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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