Gene description for PIP4K2B
Gene name phosphatidylinositol-5-phosphate 4-kinase, type II, beta
Gene symbol PIP4K2B
Other names/aliases PI5P4KB
PIP5K2B
PIP5KIIB
PIP5KIIbeta
PIP5P4KB
Species Homo sapiens
 Database cross references - PIP4K2B
ExoCarta ExoCarta_8396
Vesiclepedia VP_8396
Entrez Gene 8396
HGNC 8998
MIM 603261
UniProt P78356  
 PIP4K2B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PIP4K2B
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    GTP binding GO:0005525 IDA
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 IDA
    1-phosphatidylinositol-5-phosphate 4-kinase activity GO:0016309 IBA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    cell surface receptor signaling pathway GO:0007166 TAS
    regulation of autophagy GO:0010506 IMP
    regulation of autophagy GO:0010506 ISS
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    autophagosome-lysosome fusion GO:0061909 ISS
    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process GO:1902635 IDA
    positive regulation of autophagosome assembly GO:2000786 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    autophagosome GO:0005776 IMP
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified PIP4K2B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PIP4K2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 PRICKLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TRIM21 6737
Affinity Capture-MS Homo sapiens
4 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
5 SKP1 6500
Co-fractionation Homo sapiens
6 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SPOP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 UBQLN4 56893
Two-hybrid Homo sapiens
9 UBE2H 7328
Affinity Capture-MS Homo sapiens
10 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
11 ZRANB2 9406
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MAX  
Affinity Capture-MS Homo sapiens
13 AHCYL1 10768
Affinity Capture-MS Homo sapiens
14 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
15 ZCCHC10  
Affinity Capture-MS Homo sapiens
16 MYL9 10398
Co-fractionation Homo sapiens
17 EPB41L3 23136
Affinity Capture-MS Homo sapiens
18 BRD2  
Affinity Capture-MS Homo sapiens
19 PLCD3 113026
Affinity Capture-MS Homo sapiens
20 EDEM1  
Affinity Capture-MS Homo sapiens
21 TNFRSF1A 7132
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
22 ARL6IP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MADD 8567
Affinity Capture-MS Homo sapiens
24 PRPF8 10594
Co-fractionation Homo sapiens
25 AHCYL2 23382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CSNK2A1 1457
Two-hybrid Homo sapiens
27 PRPF3  
Two-hybrid Homo sapiens
28 TRIM55  
Two-hybrid Homo sapiens
29 TMEM194A  
Affinity Capture-MS Homo sapiens
30 DDX23 9416
Co-fractionation Homo sapiens
31 CUL4B 8450
Affinity Capture-MS Homo sapiens
32 ATG4C  
Affinity Capture-MS Homo sapiens
33 CCDC85A  
Affinity Capture-MS Homo sapiens
34 LIN7A 8825
Affinity Capture-MS Homo sapiens
35 CDCA7L  
Two-hybrid Homo sapiens
36 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ZNF428  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MED18  
Co-fractionation Homo sapiens
39 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 Pip4k2c  
Affinity Capture-MS Mus musculus
42 RNPS1 10921
Affinity Capture-MS Homo sapiens
43 SPRY4 81848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CAD 790
Co-fractionation Homo sapiens
45 INO80B 83444
Two-hybrid Homo sapiens
46 EAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
48 HMBOX1  
Two-hybrid Homo sapiens
49 RANBP2 5903
Cross-Linking-MS (XL-MS) Homo sapiens
50 ZNF131  
Two-hybrid Homo sapiens
51 KYNU  
Two-hybrid Homo sapiens
52 RPRD1B 58490
Two-hybrid Homo sapiens
53 TNFAIP8L1  
Affinity Capture-MS Homo sapiens
54 CD6 923
Affinity Capture-MS Homo sapiens
55 SCRIB 23513
Affinity Capture-MS Homo sapiens
56 PIP4K2B 8396
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
57 HSPB7  
Two-hybrid Homo sapiens
58 PTCH1  
Affinity Capture-MS Homo sapiens
59 BRD3 8019
Affinity Capture-MS Homo sapiens
60 GPBP1L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SUN2 25777
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 TRIM63  
Two-hybrid Homo sapiens
63 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PAGE1  
Affinity Capture-MS Homo sapiens
65 BRD4 23476
Affinity Capture-MS Homo sapiens
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